Plasma Lipidomics of Coronary Heart Disease Patient with Syndrome of Intermingling of Phlegm and Static Blood Based on UPLC-Q/TOF-MS / 中国实验方剂学杂志

<b>Objective::To study on the plasma lipidomics characteristics of coronary heart disease (CHD) patients with syndrome of of intermingling of phlegm and static blood, and to find differential lipid metabolites between them and healthy volunteers. <b>Method::The plasma samples from CHD patients with syndrome of intermingling of phlegm and static blood and healthy volunteers of the same age were collected. The plasma lipidomics was carried out by UPLC-Q/TOF-MS. The plasma samples were detected under positive and negative ion modes, and the primary and secondary mass spectrometry datas were collected simultaneously, and the <italic>m</italic>/<italic>z</italic> ranges were 100-2 000 and 50-2 000, respectively. The lipidomics model was established by orthogonal partial least squares discriminant analysis (OPLS-DA). Differential lipid metabolites were identified based on multivariate statistics. <b>Result::OPLS-DA model could obviously distinguish CHD patients with syndrome of intermingling of phlegm and static blood and healthy volunteers. A total of 15 plasma differential lipid metabolites were identified, such as C16 sphinganine, phytosphingosine, <italic>N</italic>, <italic>N</italic>-dimethyl-safingol, 2-hydroxyphytanic acid, orotinichalcone, PC[18∶2(2<italic>E</italic>, 4<italic>E</italic>)/0∶0], PC(0∶0/16∶0), epitestosterone sulfate, etiocholanolone sulfate, PS[22∶1(11<italic>Z</italic>)/0∶0], PC[16∶0/20∶4(5<italic>E</italic>, 8<italic>E</italic>, 11<italic>E</italic>, 14<italic>E</italic>)], PC[19∶1(9<italic>Z</italic>)/17∶2(9<italic>Z</italic>, 12<italic>Z</italic>)], PC(16∶0/0∶0), PC(18∶0/0∶0), PS[15∶1(9<italic>Z</italic>)/22∶1(11<italic>Z</italic>)]. <b>Conclusion::There are significant differences in plasma lipid characteristics between CHD patients with syndrome of intermingling of phlegm and static blood and healthy volunteers. The plasma differential lipid metabolites are helpful for the accurate differentiation of CHD patients with syndrome of intermingling of phlegm and static blood.

Brain lipidomics of intervention effect of Salviae Miltiorrhizae Radix et Rhizoma and Chuanxiong Rhizoma on ischemic stroke based on UPLC-Q-TOF-MS technique / 中国中药杂志

The aim of this paper was to investigate the intervention effect of Salviae Miltiorrhizae Radix et Rhizoma and Chuanxiong Rhizoma on brain lipid metabolism in rats with ischemic stroke. The ischemic stroke rat model was established by middle cerebral artery occlusion( MCAO) method. The brain tissues were collected after the last administration with Salviae Miltiorrhizae Radix et Rhizoma and Chuanxiong Rhizoma decoction lyophilizate. UPLC-Q-TOF-MS was used to carry out the brain lipidomics study. The lipidomics data were processed with the OPLS-DA model to find out the lipid regulation effect of Salviae Miltiorrhizae Radix et Rhizoma combined with Chuanxiong Rhizoma on ischemic stroke. The results showed that Salviae Miltiorrhizae Radix et Rhizoma and Chuanxiong Rhizoma decoction lyophilized powder can significantly alleviate brain lipidomics profiles in middle cerebral artery occlusion model rats. Eleven differential lipid metabolites in ischemic stroke model were identified. In this experiment,the protective effects of Salviae Miltiorrhizae Radix et Rhizoma and Chuanxiong Rhizoma decoction lyophilized powder on cerebral ischemia injury was verified,which might be related to the regulation of abnormal lipid metabolism.

Lipidomics study of protective effect of Salvia miltiorrhiza and Ligusticum chuanxiong on rats with focal cerebral ischemia injury based on UPLC-Q/TOF-MS / 中草药

Objective To study the regulating effect of freeze-dried substances of Salvia miltiorrhiza and Ligusticum chuanxiong Decoction on lipid metabolism abnormality in focal cerebral ischemia rats. Methods The focal cerebral ischemia rat model was established by monofilament method. The rats were randomly divided into normal group, sham operation group, model group, and drug treatment group. Plasma was collected after the last dosage and UPLC-Q/TOF-MS was used to study the plasma lipidomics. The lipidomics data were analyzed by orthogonal partial least square discriminant analysis (OPLS-DA), and the lipid metabolites changes were examined before and after the intervention of S. miltiorrhiza and L. chuanxiong. Results The focal cerebral ischemia rat model was successfully repeated. The S. miltiorrhiza and L. chuanxiong Decoction freeze-dried substances obviously reversed the abnormal lipid metabolism profile in the focal cerebral ischemia rat model. The plasma lipid biomarkers of ischemia injury rat were PS (21:0/0:0), PG (12:0/17:0), C16 sphinganine, phytosphingosine, PE [18:1 (9Z)/0:0], PC (16:1/2:0), PC (0:0/18:0), PC (16:1/0:0), PC (16:0/0:0), PC (18:2/0:0), PC (18:1/0:0), PC (18:0/0:0), and PC (20:5/0:0), respectively. Conclusion S. miltiorrhiza and L. chuanxiong Decoction freeze-dried substances have protective effects on cerebral ischemia injury, which may be related to the regulation of abnormal lipid metabolism, especially for phosphatidylcholines (PCs).

Metabonomics study of MCAO rats based on microdialysis technique combined with UPLC-Q/TOF-MS / 中草药

Objective: To study the brain striatum metabolomics characteristics of middle cerebral artery occlusion (MCAO) rats. Methods: The middle cerebral artery was occluded by monofilament to establish the MCAO rat model. The microdialysis probe was implanted into the striatum, and the brain microdialysis samples were collected under awake and free activity condition and were measured by UPLC-Q/TOF-MS in positive and negative ion mode respectively. Multivariate statistics were used to establish the metabobomics model. Results: Brain-targeted metabolomics studies based on microdialysis samples were successfully performed. Metabolomics models could clearly distinguish normal and model rats, and seven differential metabolites were identified. Conclusion: The brain metabolism characteristics of MCAO rats had changed significantly. The metabolomics research based on microdialysis technology enriches the research methods of metabolomics.