Characteristics of cervical microecology in late reproductive-age women with different grades of cervical lesions / 南方医科大学学报

OBJECTIVE@#To analyze the characteristics of cervical microecology in late reproductive-age women with cervical lesions and explore new methods for preventing cervical lesions.@*METHODS@#Cervical smears were obtained from a total of 147 women of late reproductive age, including 24 with high-risk HPV infection (HR-HPV), 27 with low-grade squamous intra-epithelial lesions (LSIL), 36 with high-grade squamous intra-epithelial lesions (HSIL), 35 with cervical cancer (CC) and 25 healthy women. llumina MiSeq sequencing of V3-V4 region of the 16S rRNA gene amplicons was used to characterize the vaginal microbiota of the women. OTUs analysis of the valid data was performed, and the α-diversity (Chao1, Simpson's Index and Shannon Index) and β-diversity (T-test, weighted UniFrac β diversity, and MetaStat analysis) were evaluated.@*RESULTS@#Dilution curve and species accumulation boxplot validated the quality of the samples. OTUs analysis of the 5 groups demonstrated that cervical bacterial genus consisted primarily of @*CONCLUSIONS@#The abundance of

Clinical study of intensity modulated radiotherapy and three-dimensional conformal radiotherapy with three-dimensional brachytherapy and concurrent chemotherapy for patients with advanced cervical cancer / 中华妇产科杂志

Objective To compare the dose, clinical efficacy and acute adverse reactions of intensity modulated radiotherapy(IMRT)and three-dimensional conformal radiotherapy(3D-CRT)combined with three-dimensional brachytherapy (3D-BT) in the treatment of concurrent radiotherapy and chemotherapy for advanced stage cervical cancer patients. Methods Data collection was performed from January 2011 to November 2015 in Chinese PLA General Hospital and Inner Mongolia Cancer Hospital.All 89 patients with advanced stage (Ⅱb-Ⅲb) cervical cancer were treated by pelvic radiotherapy and concurrent chemotherapy, 46 cases of them received IMRT and 3D-BT(IMRT group), 43 cases received 3D-CRT and 3D-BT(3D-CRT group),along with cisplatin chemotherapy.The dose accumulation of external beam radiotherapy and 3D-BT was calculated by deformable image registration to analyze clinical efficacy, acute adverse reactions and prognosis of the two groups.Results (1)Dose of radiotherapy:planning target volume(PTV)coverage of IMRT group and 3D-CRT group were respectively(95.4±4.7)% and(95.1±5.1)%, without significant differences (t=0.289, P=0.773). Compared with the patients treated with 3D-CRT, the volumn receiving at least 30 Gy (V30), V50of rectum, colon, bladder and small intestine and V20of bone marrow in the IMRT group were significantly decreased (P<0.05). Regarding the combined dose, the maximum dose (Dmax) and the minimum dose received by the most exposed 2 cm3volume of the analyzed organ(D2CC)of rectum,colon,bladder and small intestine of IMRT group were significantly lower than those of 3D-CRT group (P<0.05). (2) Short-term efficacy: the effective rate of IMRT and 3D-CRT group were respectively 93% (43/46) and 91% (39/43), with no significant differences (χ2=0.237,P=0.626). (3) Acute adverse reactions: compared with 3D-CRT, IMRT could significantly reduce grade 1-2 acute toxicity in gastrointestinal [63%(29/46) vs 84%(36/43)], genitourinary [17%(8/46) vs 37%(16/43)] and hematologic [57%(26/46) vs 79%(34/43)] system (all P<0.05). There were no significant differences of grade 3 acute adverse reactions of gastrointestinal,genitourinary and hematologic system between two groups(all P>0.05). No grade 4 acute adverse reactions were observed. (4) Prognosis: the overall survival rate at 1, 2-year of IMRT and 3D-CRT group were respectively 95.6%,89.1% and 93.1%,86.1%.The progression-free survival rateat 1, 2-year of IMRT and 3D-CRT group were 91.1%, 89.1% and 88.4%, 86.1%, respectively. There were no significant differences in overall survival rate and progression-free survival rate between two groups (P>0.05). Conclusions Compared with 3D-CRT, IMRT combined with 3D-BT has dosimetry advantages based on dose accumulation algorithms by deformable image registration. IMRT could ensure clinical efficacy and significantly reduce the incidence rate of acute toxicities.

Clinical Application of the da Vinci Robotic Surgical System for Endometrial Carcinoma / 中国微创外科杂志

[Summary] Endometrial carcinoma is one of the most common malignant tumors in female reproductive system .The treatment is given priority to with surgery .As a kind of minimally invasive surgery , the da Vinci robot surgical technique has become more and more mature in the past ten years and played an increasingly important role in gynecological oncology .As compared with the traditional laparoscopic surgery , the main advantages of the da Vinci robotic surgery system are as follows: improved three-dimensional surgical field, improved operation dexterity by mechanical wrist , and improved accuracy of operation by tremor filtering system .These innovations can help gynecological oncologists complete difficult surgery , especially for patients diagnosed of endometrial cancer with severe obesity.This article summarized the application of the da Vinci robotic surgery system in the treatment of endometrial carcinoma and compared it with the open surgery and traditional laparoscopic surgery .The new robot surgery system , innovation, applicable people and the learning curve were also involved .

Prognosis and reproductive outcome of laparoscopic intracapsular myomectomy / 南方医科大学学报

<p><b>OBJECTIVE</b>To assess the prognosis and reproductive outcomes of laparoscopic intracapsular myomectomy.</p><p><b>METHODS</b>A total of 673 women received subserosal and intramural intracapsular laparoscopic myomectomy between March, 2007 and March, 2012, and their post-operative complications, the need for subsequent surgery, symptomatic relief and reproductive outcomes were analyzed.</p><p><b>RESULTS</b>Of these patients, 42.4% had subserosal myomas and 57.6% had intramural myomas. The mean total operative time was 96∓41 min with a mean blood loss of 128∓46.2 ml, and 82.3% of the patients were discharged 48 h after the operation without early complications. A small fraction (2.3%) of the patients had a second laparoscopic myomectomy for recurrent fibroids. Of the fertility-demanding women who underwent myomectomy, 71% achieved pregnancy, 49.8% underwent caesarean section, 8% had operative vaginal deliveries, and 42.2% had spontaneous deliveries; uterine rupture occurred in none of the cases.</p><p><b>CONCLUSION</b>Laparoscopic intracapsular myomectomy, by preserving the fibroid pseudocapsule and myometrial integrity, has no early postoperative complications and ensures good fertility rates and reproductive outcomes.</p>

APPLICATION OF ATLAS~(TM)HUMAN EXPRESSION ARRAY TO ASSESS DIFFERENTIAL GENE EXPRESSION IN ENDOMETRIAL CARCINOMA / 解放军医学杂志

Atlas Human cDNA expression array technique was employed to study the differences in gene expression in normal and endometrial cancer tissues. In this technique, dATP? 32 P labeled cDNA from normal and tumor tissues was hybridized to identical human cDNA expression array membranes (containing 588 known genes). The results showed that the expression of endometrial cancer associated genes including IRP, Wnt 5, 5T4 oncofetal antigen precusor , and CD147, were higher compared with controls. It suggested that the differential hybridization technique of Atlas Human cDNA expression array can be a useful method to identify genes differentially expressed either in normal or endometrial cancer tissue.

Application of mRNA differential display technique in screening related gene of endometrial carcinoma / 中华妇产科杂志

Objective To screen related gene of endometrial cancer by mRNA differential display (DD) polymerase chain reaction (PCR). Methods Individual mRNA from normal and neoplastic endometrium obtained from the same patient were comparatively studied by DD methodology.The mRNAs were isolated from the tissues, reverse transcribed to cDNA, and then amplified by PCR. The PCR products were displayed on a polyacrylamid gel by silver staining, and the differentially expressed bands were retrieved and reamplified. Subsequently we selected four bands for sequencing and making further investigation. Results cDNA homology was analysed by searching through GenBank sequence databases and indicated that tumor fragment (T1 7) has no significant sequence homology with any sequence of known function. Acession number is AF315581. Further study showed that T1 7 expressed more abundantly in endometrial cancer than hyperplasia endometrium, but not expressed in normal endometrium. Conclusions T1.7 is a new expressed sequence tag. It may be related gene fragment of endometrial cancer.

Application of minimal invasive radical hysterectomy with laparoscope in uterine malignancy / 解放军医学杂志

Objective To investigate and analyze the clinical value of minimal invasive surgery with laparoscope for radical hysterectomy and pelvic lymphadenectomy for the treatment of cervical and endometrial carcinomas in early stage. Methods A retrospective analysis was carried out based on the clinical data of 64 patients with FIGO stage Ⅰ-Ⅱ malignant uterine tumor, among them 34 were operated with the aid of laparoscope and 30 were operated with laparotomy for radical hysterectomy and/or pelvic lymphadenectomy. Comparison was done between the two groups on operative time, blood loss, complications and postoperative recovery. Results There was no significant difference in the incidence of complications and operation time between laparoscopic radical hysterectomy and pelvic lymphadenectomy for the treatment of the patients diagnosed with early stage of cervical and endometrial carcinomas compared with those with traditional radical hysterectomy and pelvic lymphadenectomy through laparatomy. Laparoscopic radical hysterectomy and pelvic lymphadenectomy for the treatment of early stage of cervical and endometrial carcinomas resulted in less blood loss (247.13?127.62ml vs 904.51?428.37ml, P

Expression of LRP16,E-cadherin,ER and PR and their significance in endometriosis / 解放军医学杂志

Objective To study the expression of LRP16 and E-cadherin(E-cad),ER,PR in eutopic endometrium and ectopic endometrium of endometriosis(EM),as well as to study the relation one another and the possible clinical significance.Methods The expression of LRP16 and E-cadherin,ER,PR in endometrium was detected by immunohistochemical two step staining methods in 72 patients with endometriosis which was finally confirmed by laparoscopic operation.Results The expression of LRP16 in epithelioglandular endochylema in eutopic endometrium of EM was significantly higher than that in ectopic endometrium(P