RESUMO
Internship plays an important role in the establishment of clinical thinking and humanistic care and contributes to professional knowledge acquisition for the medical students of "5+3" integrative mode.Diseases which belong to general surgical department are most common in daily medical works,and the general surgical department is closely related to other departments.Therefore,the internship in general surgical department has a profound influence on medical students.For the problems prevalent in general surgical interns,such as lack of self-study ability,reclining on books heavily,as well as lack of clinical thinking and humanistic care,solutions are put forward such as establishing learning groups and peer education mode,adopting CBL teaching mode about doctor-patient communications,and improving assessment methods,which help to enhance the abilities of fundamental theory acquisition,clinical operation as well as the communication between doctors and patients for the medical students.
RESUMO
Objective To investigate the life quality of elderly patients who have received percutaneous coronary artery stent implantation,and to analyze the influence factors.Methods By using SF-12 scale and general information questionnaire,the investigation of the quality of life was conducted in 91elderly patients who had received percutaneous coronary artery stent implantation.Results The total score of the life quality of 91 elderly patients after receiving percutaneous coronary artery stent implantation was (68.06±17.72) points.The factors influencing the quality of life included age (statistic value=4.438,P＜0.05),number of interventional therapy (statistic value=2.916,P＜0.05),number of involved coronary artery vessels (statistic value=4.359,P＜0.05),angina after operation (statistic value=-2.343,P＜0.05),postoperative chest tightness (statistic value=-2.222,P＜0.05) and smoking (statistic value=3.013,P＜0.05).Conclusion The quality of life of the 91 elderly patients who have received percutaneous coronary artery stent implantation is at a moderate level,and it is influenced by many factors.Nursing staff should pay attention to the elderly patients after percutaneous coronary intervention and strengthen the health education guidance so as to provide theoretical basis for improving the quality of life of elderly patients.
RESUMO
Objective: To observe the clinical effect of Acupuncture at Baihui (GV 20), Sishencong (EX-HN 1) and Anmian [Extra, locates at the midpoint between Yiming (EX-HN 14) and Fengchi (GB 20)] for primary insomnia. Methods: A total of 112 patients with primary insomnia were randomized into an acupuncture group and a medication group according to the visiting sequence, 56 cases in each group. The acupuncture group was intervened by acupuncture at Baihui (GV 20), Sishencong (EX-HN 1) and Anmian [Extra, locates at the midpoint between Yiming (EX-HN 14) and Fengchi (GB 20)], and points selected based on syndrome differentiation, while the medication group was by oral intake of zopiclone tablet. The Pittsburgh sleep quality index (PSQI) was measured before and after treatment, and the clinical efficacy was compared after 1 month of treatment. Results: After treatment, subscales of PSQI and global score in the acupuncture group were improved, and intra-group comparisons showed statistical differences (all P<0.05); subscales of sleep quality, sleep latency, sleep duration and global score of PSQI in the medication group were improved, showing statistical differences (all P<0.05). Inter-group comparison of global and subscales scores of PSQI showed statistical differences (all P<0.05). The total effective rate in the acupuncture group was 92.9%, higher than 67.9% in the medication group, and between-group comparison showed a statistical difference (P<0.05). Conclusion: Acupuncture at Baihui (GV 20), Sishencong (EX-HN 1) and Anmian [Extra, locates at the midpoint between Yiming (EX-HN 14) and Fengchi (GB 20)] as the main treatment method can produce a better effect than oral intake of zopiclone tablet.
RESUMO
Objective To detect the effects and mechanisms of CD147 in thyroid cancer cell SW579. Methods RT-PCR and Western-blot were used to determine the levels of CD147 mRNA and protein in SW579 cells after treated with CD147 specific small interference RNA (siRNA). The proliferative ratio of SW579 cells after treated with CD147 siRNA was detected by CCK-8 assay. We detected the changes of cellular mobility after treated with CD147 siRNA by transwell assay. Mobility related proteins were analyzed by western-blot. Results The proliferative ratio and the mobility of SW579 cells were inhibited significantly by down-regulation of CD147. The levels of MMP-2 and MMP-9 were lower in treated cells than untreated ones. Expression of Wave 2 and a mobility related protein were decreased in accompany with down-regulation of CD147. Conclusion Down-regulation of CD147 inhibits mobility of SW579 by suppressing MMP-2, MMP-9 and WAVE 2.
RESUMO
Objective_To investigate the effect of ginsenoside Rg1 on the spleen structure and function of aging rats and its relative mechanism.Methods_Forty SD rats were randomly divided into normal control group, aging model group (D-galactose 120 mg/kg,qd ×42 d), Rg1 intervention group(D-galactose 120 mg/kg,qd ×42 d and Rg1 20 mg/kg, from day 15th,qd ×28 d) and Rg1 control group.After finishing injections the spleen index was meas-ured, paraffin sections were then made to observe spleen microscopic structure.Senescence-associatedβ-Galactosi-dase( SA-β-Gal) stain was used to detect aging splenocytes.The proliferative capacity of splenocytes stimulated with Concanavalin A (ConA) was measured by CCK-8.The content of IL-2,IL-6 and advanced glycosylation end products(AGEs) was detected by ELISA.The level of ROS was analyzed by flow cytometry(FCM).Malondialde-hyde(MDA), superoxide dismutase (SOD) were detected by enzymatic assay.The expression of senescence-associ-ated protein P53,P21 and RB were detected by Western blot analysis.Results_Comparing the Rg1 intervention group with the aging model group, spleen index, splenic white pulp area proportion, the proliferative capacity of splenocytes were significantly increased (P<0.05);The secretory capability of IL-2 and IL-6, the active content of SOD were obviously increased(P<0.01);The percentage of SA-β-Gal positive splenocytes, the productions of ROS and MDA were significantly decreased (P<0.01);The production of AGEs was decreased (P<0.05);The expressions of P53,P21 and Rb were also significantly down-regulated ( P<0.01) .Conclusions_Ginsenoside Rg1 relieves injure of the spleen in aging rats induced by D-galactose.It is suggested that the mechanism may be Rg1 in-hibiting oxidative stress and down-regulating P53-P21-RB signaling pathway.
RESUMO
The total RNA was extracted from ginseng leaves of Panax ginseng. The Cu/Zn-SOD gene was amplified via RT-PCR and the pET-28(a)-Cu/Zn-SOD expression vector was constructed. The pET-28 (a)-Cu/Zn-SOD recombinant plasmid was transformed into Escherichia coli BL21 (DE3) competent cells and was induced by IPTG in order to select optimal induction of expression conditions. The target protein was purified by the nickel ions (Ni ) affinity chromatography and the target protein enzyme activity was determinated by the xanthine oxidase method. The similarity of the Cu/Zn-SOD gene sequences and the Cu/Zn-SOD gene sequences of Korean ginseng in NCBI was 99. 00%. The target protein expression level was about 44.42%, and the molecular weight was 16.30 kDa after the pET-28(a)-Cu/Zn-SOD recombinants were induced by IPTG. The purified Cu/Zn-SOD protease activity reached 10,596.69 U x mg(-1). The P. ginseng pET-28(a)-Cu/Zn-SOD prokaryotic expression vector was built by the method of molecular biology, which provided the foundation for studying the Cu/Zn-SOD biology function.