Genetic diagnosis of 6 patients with fibrous dysplasia of bone / 中华内分泌代谢杂志

To explore the detection of GNAS1 gene mutation from peripheral blood and non-involved bone marrow in patients with fibrous dysplasia of bone(FD). Both peripheral blood and non-involved bone marrow collected from six FD patients were used for genetic diagnosis by next generation sequencing and direct sequencing respectively. Two patients were found to have common gene mutation(positive) by next generation sequencing, accounting for 33.3% of total six people: p.R201H(CGT>CAT) was detected in both peripheral blood and bone marrow of patient No.3, and p.R201C(CGT>TGT) was detected in both tissues of patient No.6. While direct sequencing only found p.R201H(CGT>CAT) mutation in peripheral blood and bone marrow of patient No.3. Detection of GNAS1 gene mutation may contribute to the diagnosis of FD. Hot spot mutations of GNAS1 are present in peripheral blood and non-involved bone marrow samples from some Chinese FD patients, but the diagnostic value and sensitivity of GNAS1 gene mutation in non-involved tissues of FD patients is low by next generation sequencing or direct sequencing.

Prevalence rate of healthcare-associated infection in patients in a tertiary first class military hospital / 中国感染控制杂志

Objective To investigate the current situation and related risk factors of healthcare-associated infection (HAI),so as to provide evidence for making prevention and control measures of HAI.Methods On November 26, 2014,a combination method of bedside visiting and medical record reviewing was adopted to survey HAI status, pathogen examination,and antimicrobial application in all hospitalized patients in a tertiary first class military hospi-tal.Results A total of 1 657 hospitalized patients were investigated,66 patients developed 71 times of HAI,HAI rate and HAI case rate were 3.98% and 4.28% respectively.The top 4 departments with HAI prevalence rates were departments of neurosurgery (24.49%),hematology(19.05%),cadre ward(13.73%),and burn surgery (10.91 %).The top 5 HAI sites were lower respiratory tract (40.85%),urinary tract(23.94%),upper respirato-ry tract(12.68%),surgical site(9.86%),and gastrointestinal tract(5.63%).Of 66 cases of HAI,39 (59.09%) patients sent specimens for culture,a total of 48 pathogens were cultured,the major isolated bacteria was Esche-richia coli (n = 10,20.84%),followed by Staphylococcus aureus ,Klebsiella pneumoniae ,and Pseudomonas aeruginosa ,each was 7 (14.58 %)respectively.The usage rate of antimicrobial agents was 34.40%,specimen de-tection rate in patients receiving therapeutic and therapeutic+prophylactic antimicrobial agents was 59.28%.Risk factors for HAI were age 60 years,with respirator,tracheotomy,urinary tract catheterization, arteriovenous catheterization,hemodialysis,and surgery,difference was significant(all P <0.05).Conclusion Mo-nitoring on key departments and key sites of HAI should be strengthened,antimicrobial agents should be used rationally based on pathogenic detection results,specimen pathogenic detection rate should be improved,and effective prevention and control measures needs to be taken according to the risk factors of HAI.

Effects of sulforaphane on brain Aβ deposition and oxidative stress in Alzheimer's disease mice model / 中华老年医学杂志

Objective To investigate the interventional effect of sulforaphane (SFN) on brain Aβ deposition and oxidative stress in Alzheimer's disease model mice.Methods Eight-week old C57BL/6J mice were randomly divided into three groups (n =10,each).Mice in two groups (intervention and model)were administered by drinking water containing 0.4g aluminum /100ml water and subcutaneous injection with 200mg/kg D-galactose every other day,added with 25mg/kg SFN to the intervention group or with equivalent double-distilled water to model group by a single oral gavage daily.And equivalent vehicle were given to the control mice.After 90 days,the aluminum levels in blood,brain Aβ depositions,and indices of oxidative stress in cerebral cortex were investigated.Results Blood aluminum level was higher in the AD model and SFN intervention groups than in controls(P＜0.01),and was lower in SFN intervention group than in AD model(P＜0.01).As compared to controls,the AD model showed that brain Aβ deposition was significantly increased,GPX activity was decreased and carbonyl level was remarkable increased(P＜0.05).There were no significant differences between intervention and control group in brain Aβ deposition,GPX activity and carbonyl contents (P＞0.05).Brain Aβ deposition was lower in intervention than in model group(P＜0.05).There was no significantly differences in brain SOD activity and total hydrosulfuryl content among three groups(P＞0.05).Conclusions Sulforaphane can reduce the aluminum level in blood,attenuate Aβ deposition,and regulate the oxidative stress in cerebral cortex in AD model mice.