Detection of 13q14 deletion in chronic lymphocytic leukemia by using probes D13S319 and D13S25 / 中国实验血液学杂志

To investigate the incidence of 13q14 deletion [del (13q14)] in chronic lymphocytic leukemia (CLL), Spectrum Orange labeled sequence-specific DNA probes D13S319 and D13S25 for 13q14 and interphase fluorescence in situ hybridization (I-FISH) were applied to detect del (13q14) in 24 patients with B-CLL. The results showed that among 24 patients, 10 patients (41.7%) had del (13q14) with D13S319, 11 patients (45.8%) had del (13q14) with D13S25, and 9 patients (37.5%) had del (13q14) with both D13S319 and D13S25. The incidence of del (13q14) in Binet stage A, B, C was 4/7 (57.1%), 3/7 (42.9%) and 5/10 (50.0%) respectively by D13S319 probe detection, and there was no significant difference between three Binet stages (P>0.05). It is concluded that the region of loss at 13q14 locates between D13S319 and D13S25 in CLL, and the I-FISH is a rapid and sensitive technique for analysis of del (13q14) in CLL.

A case report of misdiagnosed mantle cell lymphoma / 中国实验血液学杂志

Mantle cell lymphoma (MCL) is a rare group of non-Hodgkin's lymphoma (NHL), which is difficult to discriminate from other subtype of small lymphocytic lymphoma in morphologic appearance. In order to enhance the understanding of MCL, a case of MCL first diagnosed as follicular lymphoma (FL) was reported. The clinical and laboratory characteristics of MCL were analyzed and summarized. The results showed that the findings of flow cytometry (FCM) and immunohistochemical staining technique were compatible with the diagnosis of MCL. Cytogenetic analysis can detect multiple types of chromosomal abnormalities, including t (11; 14). In conclusion, MCL is a disease which diagnosis is difficulty confirmed. Interphase fluorescence in situ hybridization, multiplex fluorescence in situ hybridization, FCM and immunohistochemical staining technique play important roles in the diagnosis of MCL.