RESUMO
Objective To study the correlation of uterine segment thickness and time limits for patients with prior cesarean section. Methods A total of 80 women with the first pregnancy after cesarean section in our hospital from April 2013 to February 2015,according to the interval of cesarean section and second pregnancy,were divided into group A (18 cases,interval ≤3 years),group B (23 cases,an in-terval of 3 to 6 years),group C (27 cases,an interval of 7 to 9 years),and group D (12 cases,the interval ≥9 years).The uterine segment thickness and the success rate of vaginal trial production were compared.Surgical failures underwent vaginal trial production due to cesarean section were observed.Results The uterine segment thickness values of group A and group B in were respectively (5.93 ±0.34)mm and (5.45 ±0.27)mm,which were significantly higher than group C (3.24 ±0.52)mm and group D (2.83 ±0.56)mm,the difference was statistically significant (P <0.05).The vaginal trial production success rates of group A and group B were respectively 88.89% (16 /18) and 78.26% (18 /23),which were significantly higher than group C 44.44% (12 /27)and group D 33.33% (4 /12)(P <0.05).The dos-age of oxytocin of group A was (35.34 ±4.32)mL,blood loss was (256.32 ±34.21)mL, The oxytocin hormone dosage of group B was (37.09 ±4.52)mL,blood loss was (260.11 ±35.53)mL,which were lower than those in group C and group D (P <0.05).The postpartum hemorrhage rate of group A,group B and group C was respectively 0(0 /2),20.00% (1 /5),33.33% (5 /15)lower than group D 87.50%(7 /8)(P <0.05).Conclusion Maternal uterus at cesarean section within 7 years about the degree of recovery are in good condition,the higher uterine segment thickness,this time period is good for subsequent pregnancy and childbirth.
RESUMO
Objective To develop multiplex polymerase chain reaction (PCR) combined with reversedotblothy bridization (RDB) method for detection of DNA virus in respiratory samples,and provide a surveillance and rapid diagnosis tool of acute viral respiratory infection.Methods We designed multiple PCR primers and the probes referenced to virus nucleic acid sequences in the National Center for Biotechnology Information (NCBI) database,and fixed specific oligonucleotide probes on the nylon membrane.After multiple PCR amplification of virus DNA of human bocavirus (hBOV),karolinska Institutet (KI),adenovirus (AdV),Washington University polyomaviros (WUPyV),and human parvovirus B19 (HPVBI9),the denaturalized amplification products were hybridized with various specific probes,followed by visualization and analysis of the results.The sensitivity and specificity were tested.At the same time,108 cases of clinical specimens of multiple PCR products were analyzed by reverse spot hybridization detection,and compared to the results of culture method.Results The specific probes of multiple PCR-RDB only hybridized with corresponding amplification products without cross-hybridization reaction with other pathogen.The sensitivity of RDB hybridization was 1 colony-forming units (CFU).The positive rate of 34.26％ (37 cases out of 108 cases) with PCR-RDB method was significandy higher than that 27.78％ (30 cases out of 108 cases) with common test method.Conclusions The multiplex PCR combined with RDB might become a rapid and simple method to detect the DNA virus in respiratory samples,which might be a promising tool for clinical application.