RESUMO
Objective To analyze the expression and clinical significance of PIK 3CA protein in squamous cell carcinoma of the esophagus .Methods Forty -five samples of surgical resection of esophageal squamous cell carcinoma were collected as the observation group ,and 45 normal tissues adjacent to the carcinoma were selected as the control group.The expression of PIK3CA protein in the tissues of esophageal squamous cell carcinoma and the corresponding para cancerous tissue was detected by immunohistochemistry ( IHC).Results The results of IHC showed that the positive expression rate of PIK 3CA protein in esophageal squamous cell carcinoma was 53.33%, which in adjacent normal tissues was 0.00%,the difference was statistically significant (χ2 =32.727,P<0.01).The positive expression rate of PIK3CA protein was correlated with lymph node metastasis ,differentiation and clinical stage of esophageal squamous cell carcinoma (χ2 =5.180,8.201,13.566,P=0.023,0.017,0.001),and was not correlated with the degree of tumor invasion (χ2 =0.952,P =0.329).Conclusion The expression of PIK3CA protein is abnormal in esophageal squamous cell carcinoma ,and the expression of PIK3CA protein plays a role in the occurrence and development of esophageal squamous cell carcinoma .
RESUMO
Objective To investigate the clinical value of endoscopic ultrasonography (EUS)on minute gas-tric stromal tumor (MGST).Methods 90 patients diagnosed with MGST were randomly divided into two groups. 45 patients in A group were given conventional endoscopic diagnosis,45 cases in B group were treated with endoscopic ultrasound diagnosis.After the two groups of patients with endoscopic treatment,the coincidence rate of preoperative diagnosis and pathological inspection test results was recorded,clinical features in MGST under EUS diagnosis were analyzed.Results All patients were diagnosed in the case of endoscopic surgery,endoscopic submucosal dissection (ESD)accounted for 94.44%,which was significantly higher than the use of endoscopic full -thickness resection (EFR)percentage of 5.56%,and the difference was statistically significant (χ2 =14.22,P <0.05);The two groups of patients after endoscopic treatment,were diagnosed as pathological inspection MGST.Preoperative diagnosis showed that EUS MGST suspected cases of 40 patients,conventional endoscopy MGST suspected cases of 31 cases.The diag-nosis coincidence rate of B group (88.89%)was significantly higher than that in A group (68.88%),the difference was statistically significant (χ2 =5.40,P <0.05);MGST of EUS images could be clearly observed in patients with tumor morphology,size,location,growth pattern,internal parenchyma,echo pattern,strengthening methods,the compo-sition ratio in line with the results of endoscopic treatment,diagnosis results in line with the pathological diagnosis. Conclusion EUS has significant value for the diagnosis of MGST,it has advantages in the clinical guidance and treatment.