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Objective To evaluate the role of three dimensional (3D) speckle tracking echocardiography (STE) in detection of subclinical left ventricular (LV) systolic dysfunction in patients with aortic stenosis (AS).Methods The study included 47 patients with AS and 50 age-matched healthy individuals.Conventional echocardiographic parameters were measures,and 3D Speckle tracking imaging of the peak systolic strain was recorded.Results Three-dimensional global longitudinal strain (3D-GLS) were significantly reduced in patients with AS compared to controls (P ＜ 0.01).Three-dimensional global circumferential strain (3D-GCS) were significantly increased (P ＜ 0.05).Three-dimensional global area strain (3D-GAS) and three-dimensional global radial strain (3D-GRS) were similar between two groups (P ＞0.05).Correlation analysis showed that |3D-GLS| was negatively correlated with left ventricular mass index (LVMI) (r =-0.59,P ＜0.05) irrespective of |3D-GAS|,|3D-GCS|,3D-GRS,and left ventricular ejection fraction (LVEF).Conclusions Patients with AS have evidence of subclinical LV systolic dysfunction despite preserved EF％.3D speckle tracking appears is useful in detection of subclinical LV dysfunction in patients with AS.
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<p><b>OBJECTIVE</b>To investigate the effect of CD59 gene inhibition mediated by RNA interference on the proliferation and apoptosis of non-small cell lung cancer (NSCLC) GLC-P cells in vitro.</p><p><b>METHODS</b>Recombinant plasmids for RNA interference of CD59 gene were constructed and transfected into GLC-P cells via lipofectamine 2000. The stably transfected cells were examined with real-time RT-PCR, MTT assay and enzyme-linked immunosorbent assay to investigate the changes in cell proliferation and apoptosis.</p><p><b>RESULTS</b>Compared with the control cells, the cells transfected with CD59-siRNA showed significantly decreased expression levels of CD59 mRNA (P<0.05) and significantly inhibited cell proliferation.</p><p><b>CONCLUSION</b>CD59 gene is highly expressed in NSCLC and RNA interference-mediated CD59 silencing can strongly inhibit the proliferation and induce apoptosis in GLC-P cells, which shed light on a potentially new target for targeted gene therapy of NSCLC.</p>
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Humanos , Apoptose , Antígenos CD59 , Genética , Carcinoma Pulmonar de Células não Pequenas , Patologia , Linhagem Celular Tumoral , Proliferação de Células , Ensaio de Imunoadsorção Enzimática , Terapia Genética , Interferência de RNA , RNA Mensageiro , RNA Interferente Pequeno , TransfecçãoRESUMO
Purpose To investigate the expression of EZH2 and p53 protein in breast cancer and to analyze their relationship with the clinical pathologic characteristics and prognosis. Methods The expression of EZH2 and p53 protein were detected by immunohisto-chemical method in 50 cases of breast adenosis tissues, 92 cases of breast invasive lobular carcinoma ( ILC) and 200 cases of breast in-vasive ductal carcinoma ( IDC) , and their correlation was also analyzed. Results There was no statistical significance of EZH2 be-tween ILC and IDC (P>0. 016 7), while its expression in breast adenosis tissues was lower than that in ILC and IDC (P0. 016 7), while its expression in IDC was higher than that in ILC and breast adenosis tissues (P<0. 016 7). Its expression had no related to patient age, menopausal status, tumor size, lymph node metastasis in breast cancer, but related to histological types, pTNM stage, molecular subtype and sur-vival status (P<0. 05). The Kaplan-Meier survival analysis showed the expression of EZH2 and p53 had correlated with disease-free and overall survival rates of breast cancer (P<0. 05). Multivariate COX regression analysis showed that the expression of EZH2 and p53 were independent affecting factors to breast cancer patients. Conclusion The expression of EZH2 and p53 protein increase in the breast adenosis, ILC and IDC gradually, and they have positive correlation. The expression levels of EZH2 and p53 protein have im-portant value to evaluate the prognosis of breast cancer patients.
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Purpose To investigate the clinicopathological characteristics and the survival outcomes of invasive lobular carcinoma. Methods A retrospective analysis of 98 patients with invasive lobular carcinoma and 530 invasive carcinoma of no special type was performed in order to observe the histological features and the clinical outcomes of invasive lobular carcinoma. Results Median follow-up was 68. 5 months for invasive lobular carcinoma and 67 months for invasive carcinoma of no special type. Invasive lobular carcinoma presented with a larger tumor size, more histopathological grade 2 tumors, increased rate of hormonal receptor positivity, human epider-mal growth factor 2 (HER-2) negativity, and had a lower proliferative index as compared to invasive carcinoma of no special type, more frequently presented with the luminal A subtype (P<0. 001). The classical invasive lobular carcinoma presented with a smaller tumor size, to have a lower histological grade and proliferative index compared to the non-classic type, and more frequently presented with the luminal A subtype, whereas the non-classic invasive lobular carcinoma patients more frequently presented with the luminal B, HER-2 overexpression, or triple negative subtype (P=0. 035). A statistically significant difference in the outcome was observed at un-ivariate analysis for patients with non-classic for disease-free survival (P=0. 043) and for overall survival (P=0. 048), as compared with patients with classical invasive lobular carcinoma. The disease-free survival difference between the invasive lobular carcinoma and the invasive carcinoma of no special type was not significant (P=0. 537), and the overall survival rates were not statistically different between the two groups (P=0. 397). A statistically significant difference of overall survival was observed at multivariate analysis for patients with HER-2 positive and triple negative subtypes versus patients with luminal A invasive lobular carcinoma (P=0. 015, P=0. 016) . Conclusions The outcome of invasive lobular carcinoma is significantly correlated with histological and immunohistochemi-cally defined molecular subtypes. New tailored strategies should be explored in these subgroups of patients with poor outcome.
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<p><b>OBJECTIVE</b>To investigate the presence of miRNA-144 in the saliva of patients with esophageal cancer and its value for early diagnosis of esophageal cancer.</p><p><b>METHODS</b>Saliva samples were collected form patients with esophageal cancer admitted in the Fourth Affiliated Hospital of Jinan University and the First Affiliated Hospital of Guangzhou Medical College between January, 2011 and May, 2013, with saliva samples from 50 middle-aged healthy volunteers matched for age and gender ratio as the control group. The contents of miRNA-144 in the samples were detected with RT-PCR.</p><p><b>RESULTS</b>The levels of miRNA-144 in both the whole saliva and saliva supernatant were significantly higher in esophageal cancer group than in the control group (P<0.05). In the whole saliva, the cut-off point of miRNA-144 was ≥100, with a sensitivity of 74.6% and a specificity of 92.0% for esophageal cancer diagnosis (Az=0.865); in saliva supernatant, the cut-off point was ≥20 with a sensitivity of 53.7% and a specificity of 94.0% (Az=0.754), suggesting a moderate diagnostic value of miRNA-144 in whole saliva and saliva supernatant.</p><p><b>CONCLUSION</b>miRNA-144 is highly expressed in the saliva of patients with esophageal cancer and can be used as a genetic marker for early diagnosis of esophageal cancer.</p>
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Humanos , Pessoa de Meia-Idade , Biomarcadores Tumorais , Diagnóstico Precoce , Neoplasias Esofágicas , Diagnóstico , MicroRNAs , Saliva , Química , Sensibilidade e EspecificidadeRESUMO
ObjectiveTo investigate the learning curve of total thoracoscopicy cardiac surgery.MethodsClinical data of a succession of 125 patients undering total thoracoscopicy ASD and VSD repair between October 2004 to January 2010 were collected and reviewed.The procedure was perfomed by the same surgeon.The patients were divided equally into 5 groups (groupA,B,C,D and E,n =25 in each group ) according to the sequence of the operation.The operative time,extracorporeal circulation time,aortic cross-clamped time,the rate of conversion rate to thoracotomy and postoperative complications were compared between the 5 groups.ResultsThere were no statistically significant differences between the 5 groups with respect to age,gender,weight,dieases and surgical approach(P ＞ 0.05).The operative time,extracorporeal circulation time and aortic clump time in group A and group B significant longer then that in group C,group D and group E(P ＜0.05).Group A and group B was no statistically significant difference each other( P ＞ 0.05 ).Group C,D and E have no statistically significant differences between(P ＞0.05 ).The rate of conversion rate to thoracotomy and postoperative complications in 5 groups have no statistically significant differences comparative( P ＞ 0.05 ).ConclusionThe learning curve of total thoracoscopicy surgery is approximalely 50 cases.
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Objective To probe the feasibility of video-assisted thoracoscopic operation for congenital heart diseases.Methods From October 2004 to August 2008,156 patients with congenital heart disease,including 79 cases of atrial septal defect(ASD),65 cases of ventricular septal defect(VSD),1 case of aortic aneurysm rupture combined with VSD,3 cases of double chamber right ventricles(DCRV) combined with VSD,and 8 cases of partial anomalous pulmonary venous drainage,were treated totally under thoracoscope.Surgical procedures were performed through 3 trocars inserted at the right chest wall,and catheters were placed in the right femoral artery and vein to set up extracorporeal circulation.The ascending aorta was cross-clamped with long tailor-made forceps and the myocardium was protected by coronary perfusion with cold crystalloid cardioplegia. Results All the thoracoscopic operations were completed successfully.The mean extracorporeal circulation and cross-clamping time were(74?28) min and(29?13) min respectively.Postoperative ventilation was withdrawn in(3.2?1.1) h,and the patients were discharged from the hospital in(6.1?1.2) d.Thirteen of the patients had postoperative complications,including 7 cases of right pneumothorax(healed by thoracentesis),and 6 cases of fat liquefaction of the incision at the right axillary(delayed healing).No severe complications occurred in this series.UCG performed 5-7 days after the operation revealed no residual shunt of ASD or VSD;in the patients who had aortic aneurysm rupture combined with VSD,slight aortic valve regurgitation was detected after the neoplasty;and in the 3 patients with DCRV combined with VSD,the right ventricular outflow tract was patent.ECG showed sinus rhythm without AVB in the patients.Follow-up up to 3 months to 3 years were available in 132 cases(over 12 months in 95 cases).During the period,no patient had complaints;and the heart function was confirmed as level Ⅰ.Conclusions Video-assisted thoracoscopic cardiac surgery is feasible,safe,and minimal invasive for patients with VSD or ASD,resulting in quick recovery and good cosmetic outcomes.
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Objective To investigate the antioxidative effect of polypeptide from Chlamys farreri (PCF) on HaCaT cells damaged by ultraviolet B (UVB). Methods HaCaT cells model established by UVB irradiation were randomly divided into seven groups. After pretreatment with different concentrations of PCF for 1 h, the cells were irradiated by UVB at a dose of 30mJ/cm~ 2, and followed further incubatiou for 18 h. Enzyme activities including superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) were determined by biochemical methods. Total antioxidative capacity (T-AOC) and malondialdehyde (MDA) were also determined. Results PCF could enhance the activities of SOD, GSH-Px and CAT; increased T-AOC and inhibited MDA formation. Conclusion These results indicated that PCF exhibited protective effects on HaCaT cells irradiated by UVB owing to its antioxidative action.
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Objective To study the radioprotective effect of polypeptide from Chlamys farreri (PCF) on ultraviolet B(UVB)-irradiated murine thmocytes in vitro. Methods The murine thymocytes were exposed to UVB radiation. MTT method was used to detect the cell viability. The activities of intracellular glutathione peroxidase (GSH-Px), superoxided dismutase(SOD) and catalase (CAT) were measured. Intracellular reactive oxygen species (ROS) and the effect of PCF on UVB-induced apoptosis were investigated by flow cytometry. Results PCF could greatly enhance the viability of murine thymocyte and markedly promote the activities of GSH-Px, SOD and CAT, while the amount of ROS was decreased. PCF could inhibit UVB-induced thymocyte apoptosis. Conclusion PCF has significant radioprotective effects on UVB-irradiated murine thymocytes in vitro.
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ABSTRACT AIM To investigate the effect of ch-itosan on protein kinase C (PKC) activitives and diacyl glycerol(DAG)concentration in murine peritoneal macrophages. METHODS A new ion-pair reversed-phase high liquid chromatography and ra-dioimmunoassay were used to determinate the activity of PKC and the concentration of DAG respectively. RESULTS ( 1) Chitosan induced activation and translocation of PKC in murine peritoneal macrophages. The peak time was 25 min and the activity of PKC came back the basic level at 1. 5 h. (2)Chitosan increased the production of DAG in murine peritoneal macrophages. The peak time was 30 s and the concentration of DAG came back the basic level at 3 min. CONCLUSION The im-munopoteniating effect of chitosan may be associated with the channel of DAG /PKC.