Expression and Significance of Galectin⁃3 in Colon Tissue and Serum in Ulcerative Colitis / 胃肠病学

Galectin⁃3 is an endogenous lectin with extensive immunomodulatory effects, and plays an important role in inflammatory response, autoimmunity and tumorigenesis. However, the expression of galectin⁃3 in ulcerative colitis (UC) and its relationship with disease activity of UC are unclear. Aims: To detect the expression of galectin⁃3 in colon tissue and serum in UC patients, and explore the relationship between galectin⁃3 and disease activity. Methods: Thirty⁃ three patients with UC diagnosed and treated from March 2019 to December 2019 at the Second Affiliated Hospital of Zhengzhou University were recruited, and 20 paracancerous normal tissue of colon cancer patients were served as controls. The expression of galectin ⁃ 3 in colon tissue was detected by immunohistochemistry SABC. Serum samples of 24 UC patients and 20 healthy controls were collected. Serum level of galectin⁃3 was detected by ELISA. Results: The positive expression rate of galectin⁃3 in colon tissue in UC patients was significantly lower than that in paracancerous normal tissue, and the difference was statistically significant (P<0.05). The positive expression rate in mild UC patients was higher than that in moderate to severe UC patients, and the difference was statistically significant (P<0.05). The positive expression rate in remission stage was higher than that in active stage, and the difference was statistically significant (P<0.05). Serum galectin⁃3 level in UC patients was higher than that in healthy control group, and the difference was statistically significant (P<0.05). Serum galectin ⁃ 3 level in moderate to severe UC patients was higher than that in mild UC group, and the difference was statistically significant (P<0.05). Serum galectin ⁃ 3 level in active UC patients was higher than that in remission UC patients, and the difference was statistically significant (P<0.05). Conclusions: In UC patients, the expression of galectin⁃3 in colon tissue and serum are dysregulated, and the expression of galectin⁃3 in colon tissue is decreased, especially in moderate to severe UC, while the serum galectin⁃3 level is opposite to the tissue expression.

Relationship Between IL-33/ST2 Signal Pathway and Inflammatory Bowel Disease / 胃肠病学

Interleukin-33 (IL-33), a novel identified member of IL-1 superfamily, is involved in the development and progress of various immune-related diseases, including inflammatory bowel disease (IBD), via its receptor ST2-mediated pathway, but the mechanism is not fully clarified. It has been reported that IL-33 plays a dual role in promoting inflammation and inducing protective effect in intestinal mucosa. This article reviewed the relationship between IL-33/ST2 signal pathway and IBD.

Clinical Significance of NLR and MLR in Ulcerative Colitis / 胃肠病学

Background: As a routine examination, peripheral blood leukocytes or white blood cell count is considered to be a simple biological marker for inflammatory diseases; neutrophil and peripheral blood mononuclear cells are also closely related to the activity and severity of various diseases. Aims: To investigate the clinical significance of peripheral blood neutrophil/lymphocyte ratio (NLR) and monocyte/lymphocyte ratio (MLR) in ulcerative colitis (UC). Methods: A total of 62 patients with UC from October 2017 to July 2019 at the Second Affiliated Hospital of Zhengzhou University were collected. Forty-two individuals accepting physical examination were served as control group. Neutrophil count, monocyte count, lymphocyte count, NLR, MLR were compared between the two groups, and their correlations with C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), Mayo score, UCEIS score were analyzed. ROC curve was used to analyze the efficacy of the parameters for diagnosis of UC. Results: Compared with controls, neutrophil count, monocyte count, NLR and MLR in UC patients were significantly increased (P<0.05), while lymphocyte count was significantly decreased (P<0.05). Compared with mild UC, neutrophil count, monocyte count, NLR and MLR in moderate to severe UC were significantly increased (P<0.05), while lymphocyte count was significantly decreased (P<0.05). Neutrophil count, monocyte count, NLR and MLR were positively correlated with CRP, ESR, Mayo score and UCEIS score, while lymphocyte count was negatively correlated with above-mentioned indices (P<0.05). When the cut-off value was 0.470, the sensitivity of NLR for diagnosing UC was 0.613, the specificity was 0.857, AUC was 0.731 (95% CI: 0.636-0.827); when the cut-off value was 0.439, the sensitivity of MLR for diagnosing UC was 0.629, the specificity was 0.810, AUC was 0.726 (95% CI: 0.630-0.822). Conclusions: NLR and MLR are elevated in patients with UC, and can reflect the disease activity, which may be used as a serum marker for diagnosis and evaluation of UC.