circRAF1 regulates the proliferation and apoptosis of human ovarian granulosa cells / 实用医学杂志

Objective To investigate the expression of circRAF1 in primary ovarian insufficiency(POI)and explore its effect on cell proliferation and apoptosis of human ovarian granulosa cells(GCs)line(KGN cells).Methods The expression of circRAF1 in GCs and serum of patients with normal ovarian reserve function(n = 50)and patients with POI(n = 50)were detected with RT-qPCR.The correlation of circRAF1 with ovarian reserve function indexes was analyzed.Small interfering RNA(siRNA)targeting circRAF1 was constructed and trans-fected into KGN cells,with the cell proliferation detected by CCK-8 and EdU assay,and the cell apoptosis detected by JC-1 and Tunel assay.The mRNA and protein levels of genes related to cell proliferation and apoptosis(FSHR,PCNA,Bcl-2,Casp-9,Bax)were detected by RT-qPCR and WB.Results The expression of circRAF1 decreased in GCs and serum of POI patients.The expression of circRAF1 was positively correlated with serum E2 and AMH levels(P<0.001),but negatively correlated with serum FSH and LH levels(P<0.001).At the same time,the expression of circRAF1 was positively correlated with AFC(P<0.001).Interfering with the expression of circRAF1 could inhibit the proliferation of KGN cells and promote their apoptosis.Conclusion The expression of circRAF1 in the GCs and serum of POI patients is down-regulated,which is correlated with the decline of ovarian reserve function.Interfering with circRAF1 can inhibit the proliferation of GCs and promote their apoptosis.

Preparation of Mycobacterium tuberculosis EsxV lipid nanoparticles subunit vaccine and its immunological characteristics / 生物工程学报

To prepare a lipid nanoparticle (LNP)-based subunit vaccine of Mycobacterium tuberculosis (Mtb) antigen EsxV and study its immunological characteristics, the LNP containing EsxV and c-di-AMP (EsxV: C: L) was prepared by thin film dispersion method, and its encapsulation rate, LNP morphology, particle size, surface charge and polyphase dispersion index were measured. BALB/c mice were immunized with EsxV: C: L by nasal drops. The levels of serum and mucosal antibodies, transcription and secretion of cytokines in lung and spleen, and the proportion of T cell subsets were detected after immunization. EsxV: C: L LNPs were obtained with uniform size and they were spherical and negatively charged. Compared with EsxV: C immunization, EsxV: C: L mucosal inoculation induced increased sIgA level in respiratory tract mucosa. Levels of IL-2 secreted from spleen and ratios of memory T cells and tissue-resident T cells in mice were also elevated. In conclusion, EsxV: C: L could induce stronger mucosal immunity and memory T cell immune responses, which may provide better protection against Mtb infection.

The expression and significance of CD44v17 in cervical cancer and cervical intraepithelial neoplasia / 重庆医学

Objective To analyse the expression and significance of CD44 variant (CD44v17) in the process of cervical intraepithelial neoplasia (CIN) to cervical cancer,and to explore the relationship of CD44v17 with CIN and cervical cancer.Methods The expressions of CD44v17,matrix metalloproteinase 9 (MMP9) and Ki67 in normal cervical tissue,CIN Ⅰ,Ⅱ,Ⅲ and cervical carcinoma tissues were detected by real-time quantitative RCR.The expressions of CD44v17,MMP9 and Ki67 in human cervical cancer cell lines HeLa and SiHa after transfecting with CD44v17 siRNA were detected,as well.The tumor-bearing nude mice were treated with lentivirus particles of CD44v17,and the influence of CD44v17 siRNA on tumorigenicity in nude mice was analysed.Results The expression level of CD44v17 increased gradually from CIN Ⅰ,Ⅱ,Ⅲ to cervical carcinoma tissues.Compared with normal cervical tissue,the expression levels of CD44v1 7,MMP9 and Ki67 were significantly increased in CIN Ⅱ,Ⅲ and cervical carcinoma tissues (P＜0.05),while no significant difference was found between normal cervical tissue and CIN Ⅰ tissue (P＞0.05).The expression levels of MMP9 and Ki67 in human cervical cancer cell lines HeLa and SiHa were decreased significantly after transfecting with CD44v17 siRNA (P＜0.01).The tumor volume and weight were decreased by treatment with CD44v17 siRNA transfection (P＜0.01),and the tumorigenicity in nude mice was decreased.Conclusion CD44v17 may promote the development of CIN to cervical cancer.It is expected to be an indicator in predicting malignant potential of CIN.

Effects of Core Control Training on Spastic Cerebral Palsy / 中国康复理论与实践

Objective To study the effects of core control training on gross motor function of children with spastic cerebral palsy. Meth-ods 60 children with spastic cerebral palsy were divided into control group (n=30) and treatment group (n=30). The control group accepted routine comprehensive rehabilitation therapy including physical therapy, acupuncture, electrotherapy, etc., while the treatment group accept-ed the core control training in addition. They were assessed with the Gross Motor Function Measure (GMFM-88) and the Trunk Control Test (TCT) before and 3 months after rehabilitation. Results Both groups improved in the scores of GMFM-88 and TCT after treatment (P<0.001), and improved more in the treatment group than in the control group (P<0.001). Conclusion The core control training can strengthen the trunk control and further improve the gross motor function in children with spastic cerebral palsy.

Evaluation of Physical Therapy Team Management Mode / 中国康复理论与实践

@#Objective To investigate the methods and effectiveness of the physical therapy team management mode.MethodsAccording to the type of cerebral palsy and a certain ratio of beds and staff, the therapists were divided into 3 groups, and relatively fixed. They treated children with differrent types of CP and different beds by turn. The implementation of treatment was 3 years.ResultsAfter the physical therapy team management mode was executed, the therapist's theoretical knowledge and technical skill were upgraded, patient satisfaction raised, and the occurrence of accidental injury reduced.ConclusionThe physical therapy team management mode is conducive to standardize therapist behavior, improve the capacity of theory and practice of therapists and promote patient recovery.

Effect of Body Weight Support Training on Cerebral Palsy / 中国康复理论与实践

@#Objective To observe the effect of the body weight support training (BWST) on walking ability of children with cerebral palsy (CP). Methods 50 children with CP were divided into the BWST group （n=30） and control group（n=20）. All cases were treated with physical therapy, but the BWST group were added with BWST. The scores of D and E dimensions of the Gross Motor Function Measure Scale (GMFM) were used to assess the walking ability before and after the treatment, respectively. Results The scores of D and E dimensions of GMFM improved in both groups after treatment (P<0.01), and that of the BWST group improved more than control group （P＜0.05）. Conclusion BWST can facilitate the recovery of the walking ability after cerebral palsy.