A mouse cerebral cortical microinfarct model induced by ultrashort laser irradiation wih two-photon microscopy / 国际脑血管病杂志

ObjectiveTo verify the reliability of the mouse model of cerebral cortical microinfarct induced by two-photon microscopy and to explore its pathological changes.MethodsSeventeen male C57BL/6J mice were randomly divided into a microinfarct group (n=11) or a sham operation group (n=6).A thinned cranial window of 3 mm diameter was performed over the cerebral cortex with a high-speed micro-drill until the small blood vessels were clearly observed under a dissecting microscope.Then, a permanent single cortical penetrating arteriole occlusion was induced with a gradually enhanced ultrashort laser irradiation through the thinned cranial window with two-photon microscopy.At 7 days after modeling, the cerebral microinfarct volume was measured with HE staining, and the neuron loss, activation of glial cells and deposition of 3-nitrotyrosine were assessed using immunohistochemistry.ResultsThe target vessels of cerebral cortex in 8 (72.7%) mice were occluded and the microinfarcts formed in the microinfarct group, and the average microinfarct volume was 317.23±20.29 μm3.There were remarkable neuron loss and microglia infiltration in the infarcted core, a large number of reactive astrocytes surrounding the infarcted lesion, and massive deposition of 3-nitrotyrosine in the peri-infarct area.No infarcts were observed in the sham operation group.The deposition of 3-nitrotyrosine in the sham operation group was significantly less than that in the microinfarct group (8.00±1.48 vs.98.38±9.10;t=23.962, P<0.001).Conclusions The mouse model of cerebral cortical microinfarct induced by two-photon microscopy is reliable, and its histopathologic changes are consistent with the pathologic features of cerebral microinfarct.

The Experience About Operative Treatment of Some Complicated Fistula / 昆明医科大学学报

38 cases of Vesicovaginal fistula were repaired. Among them 11 cases were Complicated V. V. F. Three operative methods were reported: Using musculus bulbocavenosus island flap, the huge Vesico-vaginal fistulae were successfully repaired. Using anastomosis of artificial fistula on the bladder with remnant urethra, the huge V. V. F with urethral trauma were repaired. Extraperitoneal and intracesical repairing cured high position and huge V. V. F. The report put forward the principlle the the simpler the method the better the result when repairing complicated V. V. F. V. V. F. could only be cured by surgical operation.