Apoptotic cells accelerate the development of systemic lupus erythematosus in lupus prone BXSB mice / 中华风湿病学杂志

Objective To probe into the role of apoptotic cells in the disease development of systemic lupus erythematosus.Methods Apoptotic thymocytes injected intraperitoneally into syngeneic normal C57 mice and lupus prone BXSB mice,and then the level of anti dsDNA and anti ssDNA antibodies of IgM and IgG classes,and the concentration of urine protein and immune complex of IgG class deposited in kidney were detected.Results The apoptotic thymocytes induced the production of anti dsDNA and anti ssDNA antibody of IgG class in C57 mice,and their levels were higher in female BXSB mice than in male ones.The concentration of urine protein of male BXSB mice injected with apoptotic thymocytes was noticeably higher than that of the control male BXSB mice.Conclusion Apoptotic cells have immunogenicity,and may be the major source of autoantigens in lupus BXSB mice.Lupus genetic factors and Yaa gene can potentiate the immunogenicity of apoptotic cells or accelerate their inducing the lupus nephritis.

T4 T8 DOUBLE MARKERS ON THE SURFACE OF THE ACTIVIATED HUMAN T CELLS / 中国免疫学杂志

T4 cells were isolated twice separately from peripheral blood of the same donor.After activation by lectin,the isolated cells were culturedwith the medium containing self-made IL-2 for a long period.The culturedcells were identified for OKT antigens by immuno-fluorescent technique onday 10 and 22 of the cultivation,and the appearance of both T4 and T8antigens on the same cell (double marker cell)could be detected in about60-80%of cultured cells.The double marker T cells decreased in numberand the percentage of single marker T cells,either T4 or T8 cells,increasedgradually along with the cultivation.The function of double marker T cellswere investigated and the suppressor activity of these cells on PWM indu-ced antibody production response by cooperation between B cells and fresh T4cells was found.These results suggest that human peripheral blood T4 andT8 cells are not terminaly differentiated cells,because T4 and T8 antigencan be coexpressed on the same cell during the earlier cultivation.Afterlong period of activation,T4 and T8 double marker cells may change intoeitherT4 or T8 cells.