RESUMO
OBJECTIVE@#To study the effect of anti-tumor peptide of tumstatin on tumor growth of human laryngeal squamous carcinoma in nude mice and the underlying mechanism.@*METHOD@#Nude mice model bearing laryngocarcinoma were established by using human laryngeal squamous carcinoma cell line (Hep-II). The animals were given tumstatin or PBS for 10 consecutive days. The volumes of the subcutaneous tumor were observed. The microstructure in which the general 2-step immunohistochemical examination was adopted and ultra-micro-structural changes of carcinoma after administration of tumstatin were observed under light and electron microscopes for pathology examination.@*RESULT@#The differences was statistically significant in the net mice weight, tumor weight, tumor volume and tumor weight/net mice weight between the treatment group and the control group (P<0.01). The restrained percentage of tumor was 51.58%. The necrosis and apoptosis of the tumor cells and the angiogenesis reduction were found under light and electron microscope in the treatment group. MVD of the treatment group was lower than that of the control group (P<0.01).@*CONCLUSION@#Tumstatin can significantly restrain the development of laryngocarcinoma.
Assuntos
Animais , Feminino , Humanos , Masculino , Camundongos , Apoptose , Autoantígenos , Farmacologia , Carcinoma de Células Escamosas , Patologia , Linhagem Celular Tumoral , Colágeno Tipo IV , Farmacologia , Neoplasias Laríngeas , Patologia , Camundongos Endogâmicos BALB C , Camundongos Nus , Peptídeos , Farmacologia , Ensaios Antitumorais Modelo de XenoenxertoRESUMO
OBJECTIVE@#To study the effects of adenovirus-mediated tissue factor pathway inhibitor-2 gene on the invasion of laryngeal squamous cancer.@*METHOD@#Ad-TFPI-2 was transfected into laryngeal squamous cancer (Hep-2) cell. Western-blot was used to test the TFPI-2 protein expression and Boyden Chamber experiment was used to examine the invasive ability of Hep-2 cells. Furthermore, the Ad-TFPI-2 infected Hep-2 cells were subcutaneously inoculated in nude mice and the tumor formation capability were observed.@*RESULT@#Ad-TFPI-2 was identified correctly by endonuclease and sequencing and the virus titer was 2.8 x 10(13) PFU/L. In the Hep-2 cells of treated group, the TFPI-2 protein expression was increased while the invasive capability was descent. The tumor formation capability was also decreased in the treated group nude mouse model.@*CONCLUSION@#TFPI-2 recombinant adenovirus can effectively inhibit the invasive capability of laryngeal squamous cancer.
Assuntos
Animais , Humanos , Camundongos , Adenoviridae , Genética , Carcinoma de Células Escamosas , Patologia , Linhagem Celular Tumoral , Terapia Genética , Vetores Genéticos , Glicoproteínas , Genética , Neoplasias Laríngeas , Patologia , Camundongos Endogâmicos BALB C , Camundongos Nus , TransfecçãoRESUMO
0.05) before treatment. After treatment, the hearing thresholds were recovered in 21 ears, signifi-cantly improved in 8 ears, improved in 4 ears and not changed in 2 ears in the DM group. In placebo group, the hearing threshold was recovered in 6 ears, signifi-cantly improved in 9 ears, improved in 15ears and not changed in 6 ears after treatment. There was a signifi-cant difference in the level of hearing improvement be-tween the DM group and the placebo group(x2=13.49, P