Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 5 de 5
Filtrar
Adicionar filtros








Intervalo de ano
1.
Journal of the Korean Neurological Association ; : 524-526, 2006.
Artigo em Coreano | WPRIM | ID: wpr-14650
2.
Korean Journal of Nuclear Medicine ; : 99-108, 2004.
Artigo em Coreano | WPRIM | ID: wpr-168772

RESUMO

PURPOSE: The ability to noninvasively track the migration of neural progenitor cells would have significant clinical and research implications. We generated stably transfected F3 human neural progenitor cells with human sodium/iodide symporter (hNIS) for noninvasively tracking F3. In this study, the expression patterns of hNIS gene in F3-NIS were examined according to the cultured time and the epigenetic modulation. MATERIALS AND METHODS: F3 human neural stem cells had been obtained from Dr. Seung U. Kim (Ajou University, Suwon, Korea). hNIS and hygromycin resistance gene were linked with IRES (Internal Ribosome Entry Site) under control of CMV promoter. This construct was transfected to F3 with Liposome. To investigate the restoration of hNIS gene expression in F3-NIS, cells were treated with demethylating agent (5-Azacytidine) and Histone deacetylase inhibitor (Trichostatin A: TSA). The expression of hNIS was measured by I-125 uptake assay and RT-PCR analysis. RESULTS: The iodide uptake of the F3-NIS was higher 12.86 times than F3 cell line. According to the cell passage number, hNIS expression in F3-NIS gradually diminished. After treatment of 5-Azacytidine and TSA with serial doses (up to 20micro M, up to 62.5nM, respectively) for 24 hours, I-125 uptake and mRNA of hNIS in F3-NIS were increased. CONCLUSION: These results suggest that hNIS transfected F3 might undergo a change in its biological characters by cell passage. Therefore, the gene expression of exogenous gene transferred human stem cell might be affected to the epigenetic modulation such as promoter methylation and Histone deacetylation and to the cell culture conditions.


Assuntos
Humanos , Azacitidina , Técnicas de Cultura de Células , Linhagem Celular , Epigenômica , Expressão Gênica , Inibidores de Histona Desacetilases , Histonas , Transporte de Íons , Lipossomos , Metilação , Células-Tronco Neurais , Ribossomos , RNA Mensageiro , Células-Tronco , Transgenes
3.
Korean Journal of Nuclear Medicine ; : 294-299, 2004.
Artigo em Coreano | WPRIM | ID: wpr-54171

RESUMO

PURPOSE: Both human NIS and mutant D2R transgenes are proposed as reporting system in transplanted cell tracking. Using hepatoma cell lines, we constructed a dual reporter system containing human sodium-iodide symporter (hNIS) and dopamine 2 receptor (D2R) and compared its characteristics. MATERIALS AND METHODS: The recombinant plasmid (pIRES-hNIS/D2R) was constructed with IRES (internal ribosome entry site) under control of the CMV promoter. pIRES-hNIS/D2R was transfected to human hepatoma SK-Hep1 cell line with lipofectamine. HEP-ND (SK-Hep1-hNIS/D2R) cells stably expressing hNIS and D2R was established by selection with G418 for two weeks. RT-PCR was performed to investigate the expression of both hNIS and D2R genes. The expressions of hNIS and D2R were measured by 125I uptake assays and receptor binding assays. Specific binding of D2R to [3H]spiperone was verified by Scatchard plot with (+) butaclamol as a specific inhibitor. K (d) and B (max) values were estimated. The correlation between hNIS and D2R expression was compared by using each clone. RESULTS: Similar quantities of hNIS and D2R genes were expressed on HEP-ND as RT-PCR assays. HEP-ND cells showed 30 to 40 fold higher radioiodine uptakes than those of parental SK-Hep1 cells. 125I uptake in HEP-ND cells was completely inhibited by KClO4, a NIS inhibitor. Specific binding to HEP-ND cells was saturable and the K (d) and B (max) values for HEP-ND cells were 2.92 nM, 745.25 fmol/mg protein and 2.91nM, 1323 fmole/mg protein in two clones, respectively. The radioiodine uptake by hNIS activity and D2R binding was highly correlated. CONCLUSION: We developed a dual positron and gamma imaging reporter system of hNIS and D2R in a stably transfected cell line. We expect that D2R and hNIS genes can complement mutually as a nuclear reporting system or that D2R can be used as reporter gene when hNIS gene were used as a treatment gene.


Assuntos
Humanos , Butaclamol , Carcinoma Hepatocelular , Linhagem Celular , Rastreamento de Células , Células Clonais , Proteínas do Sistema Complemento , Dopamina , Elétrons , Genes Reporter , Transporte de Íons , Pais , Plasmídeos , Ribossomos , Iodeto de Sódio , Sódio , Transgenes
4.
Journal of the Korean Neurological Association ; : 552-554, 2004.
Artigo em Coreano | WPRIM | ID: wpr-60337

RESUMO

Widened palpebral fissure has been regarded as one of the hallmarks of Bell's palsy. However, the palpebral fissure on the affected side may be narrower than that of the unaffected side. The narrowing of the palpebral fissure has been often thought as a sign caused by weakness of the levator palpebrae and Muller's muscles. However, weakness of the frontal muscle may cause drooping of the eyebrow, resulting in the narrowing of the palpebral fissure. We describe a patient of Bell's palsy with a narrowing of the palpebral fissure.


Assuntos
Humanos , Paralisia de Bell , Sobrancelhas , Músculos
5.
Korean Journal of Nuclear Medicine ; : 528-531, 2004.
Artigo em Coreano | WPRIM | ID: wpr-203794

RESUMO

PURPOSE: PET has some disadvantage in the imaging of small animal due to poor resolution. With the advent of microPET scanner, it is possible to image small animals. However, the image quality was not good enough as human image. Due to larger brain, cat brain imaging was superior to mouse or rat. In this study, we established the cat brain infarction model and evaluate it and its temporal change using microPET scanner. MATERIALS AND METHODS: Two adult male cats were used. Anesthesia was done with xylazine and ketamine HCl. A burr hole was made at 1cm right lateral to the bregma. Collagenase type IV 10 microliter was injected using 30 G needle for 5 minutes to establish the infarction model. 18F-FDG microPET (Concorde Microsystems Inc., Knoxville, TN) scans were performed 1, 11 and 32 days after the infarction. In addition, 18F-FDG PET scans were performed using human PET scanner (Gemini, Philips medical systems, CA, USA) 13 and 47 days after the infarction. RESULTS: Two cat brain infarction models were established. The glucose metabolism of an infarction lesion improved with time. An infarction lesion was also distinguishable in the human PET scan. CONCLUSION: We successfully established the cat brain infarction model and evaluated the infarcted lesion and its temporal change using 18F-FDG microPET scanner.


Assuntos
Adulto , Animais , Gatos , Humanos , Masculino , Camundongos , Ratos , Anestesia , Infarto Encefálico , Encéfalo , Colagenases , Fluordesoxiglucose F18 , Glucose , Infarto , Ketamina , Metabolismo , Agulhas , Neuroimagem , Tomografia por Emissão de Pósitrons , Xilazina
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA