Initial bradykinin triggers calcium-induced calcium release in C6 glioma cells and its significance / 神经科学通报·英文版

<p><b>OBJECTIVE</b>To investigate the underlying mechanism for the selective modulation of the permeability of blood-tumor barrier (BTB) by small dose of bradykinin (BK).</p><p><b>METHODS</b>C6 glioma cells were treated with BK, and changes of intracellular nitric oxide (NO) and intracellular calcium level were measured with fluorescent spectrophotometer.</p><p><b>RESULTS</b>The initial application of BK easily triggered extracellular calcium influx, which resulted in intracellular calcium store release in C6 glioma cells. The above mechanism was also named ryanodine mediated calcium induced calcium release (CICR). We also detected a long-lasting intracellular NO elevation in C6 glioma cells upon BK treatment. Further study showed that ryanodine mediated CICR contributed greatly to the secondary NO elevation induced by BK treatment.</p><p><b>CONCLUSION</b>These results suggested that BK triggered CICR in C6 glioma cells and the associated NO generation might be the underlying mechanism for the selective modulation of BTB permeability by BK.</p>

Effects of aluminum on the integrity of blood brain barrier in juvenile rats / 中华预防医学杂志

<p><b>OBJECTIVE</b>To investigate the effects of aluminum on the integrity of blood brain barrier in juvenile rats.</p><p><b>METHODS</b>The 40-day old Sprague-Dawley (SD) rats were exposed to aluminium chloride by intraperitoneal injection, at a dose of Al3+ 0 mg/kg, 2.5 mg/kg, 5 mg/kg, 10 mg/kg, respectively. Morris water amaze system was used to test the learning and memory ability. The Evans blue content in brain was analyzed after injection. The ultrastructure's change of the blood brain barrier (BBB) was observed with transmission electron microscope. The expression of occluding protein in BBB was determined by Western blot method.</p><p><b>RESULTS</b>As compared with control group, the permeability of BBB in mid-level Al and high-level Al was enhanced (P <0.01), the expression of occluding protein was descended (P <0.01). The ultrastructures of the BBB were changed. No differences between every group on learning and memory ability (P>0.05).</p><p><b>CONCLUSIONS</b>Short time and low dose of Al might not change the ability of learning and memory in juvenile rats, however the permeability and ultrastructures of the BBB might be significantly changed.</p>

Stent Implantation for Teatment of Intracranial Wide-Necked Aneurysms: 12 Cases Report / 中国康复理论与实践

@#ObjectiveTo investigate the effect of endovascular stent with or without coil graft on intracranial wide-necked aneurysms. Methods12 cases with intracranial wide-necked aneurysms, among whom, 10 cases were treated with stents and coils, 2 C4 aneurysms were treated with graft stent, were analyzed retrospectively. ResultsOf the 10 cases who were treated with stents and coils, 8 patients were completely occluded and other 2 patients were incompletely (>95%) occluded. 2 cases accepted graft stent were obliterated. Follow-up with angiography in 10 patients for 3～12 months revealed that all the aneurysms were completely occluded. 1 patient among them died. ConclusionEndovascular stent with or without coil graft is effective on intracranial wide-necked aneurysms.