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Journal of Biomedical Engineering ; (6): 305-309, 2011.
Artigo em Chinês | WPRIM | ID: wpr-306570

RESUMO

Three-dimensional poly (epsilon-caprolactone)/silk sericin (PCL/SS) porous nanofibrous scaffolds were prepared by electrospinning. The structure and properties of the scaffolds were characterized by Scanning Electron Microscope (SEM), Transmission Electron Microscope (TEM), Fourier Transform Infrared Spectroscopy (FTIR) and water contact angle instrument. Studies on cell adhension and proliferation were carried out by culturing human primary skin fibroblast cells (FEK4) on these scaffolds using SEM and MTS. The experimental results showed that the PCL/SS nanofibrous scaffolds with SS nanoparticles had porous non-woven mesh structure with nanofibrous cross-linked with each other. Fiber diameter was very uniform and precise, and the secondary structure of SS protein had not been changed. Furthermore, the capability of hydrophile increased with the SS addition, which improved FEK4 cells adhesion and proliferation on the scaffolds.


Assuntos
Materiais Biocompatíveis , Química , Adesão Celular , Células Cultivadas , Fibroblastos , Biologia Celular , Microscopia Eletrônica , Nanofibras , Química , Poliésteres , Química , Sericinas , Química , Seda , Química , Espectroscopia de Infravermelho com Transformada de Fourier , Alicerces Teciduais , Química
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