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Objective:To analyze PD-L2 expression on monocytes of peripheral blood cells in systemic lupus erythematosus ( SLE) and it′s correlation with the degree of disease activity .Methods:Peripheral blood of 26 cases of SLE patiens and 38 cases of healthy controls were collected .Peripheral blood mononuclear cells ( PBMC) were isolated and realtime PCR was carried on to analyze the PD-L2 gene expression.At the same time flow cytometry was performed to analyze the CD 14 and PD-L2 expression.Results:PD-L2 was significantly up-regulated on monocytes in RA patients than in healthy controls and had correlation with the disease activity and the SLEAI score.Conclusion:These findings help to clarify the function of PD-L2,including its potential role as a biomarker for SLE .
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ObjectiveTo explore the relationship between the expression of human proliferating cell nuclear antigen (PCNA) and human MutS honolog2 gene (hMSH2) in breast cancer and its significance. Methods PCNA and hMSH2 expression were detected in 68 cases of breast cancer tissues by immunohistochemistry.ResultsAmong the 68 cases of breast cancer, the expression rate of PCNA and hMSH2 was 44.1％ (30/68) and 54.4％ (37/68) respectively.The expression of PCNA and hMSH2 was positively correlated with lymph nodes metastasis(P ＜0.05).PCNA expression was positively correlated to hMSH2 expression in breast cancer (P ＜0.05).ConclusionInteraction between PCNA and hMSH2 may be related to the carcinogenesis of breast cancer.Effective detection of PCNA and hMSH2 proteins may contribute to the evaluation of malignancy and biological behavior of breast cancer.