RESUMO
Objective To study the process of brain protein hydrolysate of inactivate and virus removal.Methods The Parvoviridae parvovirus genera of porcine parvovirus (PPV),vesicular stomatitis virus rhabdovirus genera of vesicular stomatitis virus (VSV) were chosen as a model virus,wherein PPV represents no envelope deoxyribonucleic acid(DNA) virus,VSV represents the envelope ribonucleic acid(RNA) virus.Simulation of the production process of virus inactivation steps 100 ℃ × 30 min,ultrafiltration as inactivation/removal condition.The virus respectively according to 1 ∶ 9 into the brain protein hydrolysate,high temperature and ultra filtration virus inactivation/removal.In pig kidney cells (PK-15) in PPV cell culture,Africa green monkey kidney cells(Vero cells) cultured VSV,determination of virus titer.Results PPV and VSV through the sterilization,virus median tissue culture infective dose(TCID50) were 6.15log/0.1mL(logs),5.37 log/0.1mL(logs) ;removal processaverage virus reduction coefficient were 6.15log/0.1mL(logs),5.37 log/0.1mL(logs).Conclusion The high temperature and ultra filtration produces brain protein hydrolysate solution process are effective virus inactivation/removal process.
RESUMO
Objective To investigate the protective effects of ShenFu injection on kidney of experimental acute pancreatitis in mrs. Methods Twenty and one Wister rats were randomized into the sham-operated group,AP group and Shenfu group. The plasma level of MDA,MPO,SOD,TNF-α and the kidney level of MDA、ON、SOD and historical scores was determined. Results The values of plasma SOD、MDA、MPO、TNF-α and the kidney of MDA、ON and historical scores in Shenfu-gronp were higher than that in the sham-operated group but lower than that in AP group. The values of SOD of plasma and kidney in Shenfu group is higher than that in AP but lower than that in the sharm-operated group. Coneluion Shenfu injection can protect the early injure of kidney of severe acute panereatitis,which mechanism may be to improve the kidney microcirculation,to reduce the production of TNF-α,to decrease the inflammation of plasma and kidney and to decrease the injure of oxygen free radicals.