Bone marrow mesenchymal stem cells exosome miR-126-3p targets CCR1 to inhibit the malignant proliferation and metastasis of non-small cell lung cancer cells / 西安交通大学学报(医学版)

【Objective】 To investigate the effects of miR-126-3p targeting chemokine receptor 1 (CCR1) in exosomes derived from bone marrow mesenchymal stem cells (BMSC) on the proliferation, migration, and invasion of lung cancer cells. 【Methods】 BMSC cells were cultured; exosomes were extracted and identified by the exosomal marker proteins CD63 and TSG101. After exosome culture of A549 cells for different durations (0, 24, 48, and 72 h), cell survival rate was detected by CCK-8, mRNA levels of miR-126-3p and CCR1 were detected by qRT-PCR, and cell migration and invasion abilities were detected by Transwell assay. The relative expressions of CCR1, epithelial cadherin (E-cad), neural cadherin (N-cadherin), and Vimentin were detected by Western blotting. 【Results】 Exosomes had round or oval cup-shaped structures with bright edges and dark middle, with a particle size distribution of about 152 nm, expressing CD63 and TSG101 proteins. The expression of miR-126-3p in exosomes was higher than that in A549 cells. The expression of miR-126-3p was low in A549 cells and that of CCR1 mRNA was high. However, after co-culture with exosomes, the expression of miR-126-3p in A549 cells was increased, while the expression of CCR1 was decreased. A549 cells were cocultured with exosomes for 0, 24, 48, and 72 h. The survival rate, migration and invasion abilities, CCR1 gene and protein expression levels, and N-cad and Vimentin protein expression levels of A549 cells decreased gradually with the extension of culture time. The level of miR-126-3p and the expression of E-cad protein increased gradually with the extension of culture time. 【Conclusion】 The co-culture of exosomes derived from bone marrow mesenchymal stem cells with A549 cells can increase the expression level of miR-126-3p, and miR-126-3p can reduce the proliferation, migration, and invasion of A549 cells by targeting the inhibition of CCR1 expression.

Shear mechanical properties of lumbar trabecular bone in aged people / 中国组织工程研究

BACKGROUND:Shear mechanical properties of lumbar trabecular bone should be understood in treating osteoporosis,bone remodeling,bone reconstruction,as well as the choice of fracture fixed equipment.OBJECTIVE:To explore whether the shear mechanical properties of lumbar trabecular bone is different in young and aged normal fresh cadavers.METHODS:Experimental samples were from 4 normal fresh corpse,aged 22,28,70 and 75 years,were provided by Department of Anatomy,Norman Bethune Medical University.Spinal column thoracic vertebra L_2-L_5 specimen were obtained from corpse within 1 hour and cut into 40 min×5 mm×5 mm pieces.The specimen were tested on electronic universal testing machine with speed of 5 mm/min,the shear load,stress,strain and other data would be printed automatically,and the morphology of fracture surface was observed.RESULTS AND CONCLUSION:Majority of the fracture surfaces were comminuted,minority were transverse or oblique fracture.The macroscopic observation showed that trabecular arranged compact in the thoracic vertebra cancellous bone of the youth group,which was sparse arrangement and presented with senile osteoporosis in the aged group.The shear force,shear stress and strain of the youth group was greater than that of the aged group[(37.19±3.25),(28.34±3.42) N;(1.49+0.13),(1.13±0.14) MPa;(1.40±0.50)%,(0.90±0.20)%,P＜0.05].It demonstrated that the impact mechanics properties of thoracic vertebra cancellous bone are different between the youth and aged groups.

Stress relaxation characteristics in brachial plexus of experimental animals using function as description method / 中国组织工程研究

BACKGROUND:The brachial plexus injury anastomosis suture technique is necessary to understand the mechanics characteristic of brachial plexus nerve stress relaxation.Previous studies mainly focused on single tension mechanics of brechial plexus nerve,but few studies concerning stress relaxation and creeping elasticity.OBJECTIVE:To observe and analyze experimental animal brachial plexus nerve stress relaxation mechanics characteristic in the description manner of function,to provide experimental parameters for clinic.DESIGN,TIME AND SETTING:The experimental observations to use function as a way to describe from 1~(st) to 10~(th_ March,2007 at the Experimental Center of Jilin University.MATERIALS:The experimental male Sprague Dawley rats aged 6 months were provided by Changchun Gaoxin Medicine Animal Experimentation Center.METHODS:The stress relaxation experiment was performed in Experimental animal Brachial plexus Nerve on the the electronic universal testing machine (Shimadzu,Japan).The stress relaxation experiment strain increased speed was 1%/s,setting time of 7 200 seconds.100 empirical data were collected and disposed by one-way linear regression analysis.MAIN OUTCOME MEASURES:The stress relaxation data and curve,the Stress and time change rule were measured.RESULTS:The stress relaxation quantity of animal brachial plexus nerve test specimen at 7 200 s was 0.316 MPa.Stress relaxation curve changed with logarithm.Stress relaxation changed rapidly at 600 seconds,decreased gradually,and reached a balance level at 7 200 seconds.CONCLUSION:The stress relaxation curve changed with the logarithm relations.Brachial plexus has visco-elastic mechanical properties.

Dynamics of Liver Granuloma and the Relative Changes of Th1/Th2 Cytokine Levels in Mice Infected with Schistosoma Japonicum / 复旦学报（医学版）

Purpose To oberve the dynamics of liver granuloma and the relative changes of Thl/Th2cytokine levels in mice infected with Schistosoma japonicum, and investigate the role of Th1/Th2 in S.japontcum granuloma formation and regulation. Methods Liver granuloma measurement were performedby histological examination and the ELISA were used for the quantitative determination of IL-2, IFNr andIL-4 in murine serum and spleen lymphocyte culture medium at 0,4,6,8,10 and 12 wk after infection.Results At 6 wk liver granuloma formation appeared and at 8 wk liver granuloma peaked. After 12 wk livergranuhoma diminished obviously. Meanwhile, at 4 - 6 wk IL-2, IFNr and IL-4 began to rise, at 8 wk thelevels of Th1 cytokines IL-2 and IFNγ peaked and then declined, and at 8 wk the levels of Th2 cytokines IL-4 were rapidly enhanced and increased obviously with a prolongation of the infection duration.Conclusions The Th1cytokines IL-2 and IFNγ were correlated well with S. japonicum granulomaformation and vigour, and the Th2 cytokines IL-4 might play an important role in down-regulating egggranuloma reaction at chronic schistosomiasis by inhibiting the Th1cytokines.

Inducement of tetraploid Dioscorea zingiberensis / 中草药

Objective To create new tetraploid resource of Dioscorea zingiberensis in the hope of potential breeding materials with high yield and high level of diosgenin. Methods Callus with tiny green buds were directly soaked in colchicine solution or cultured in medium plus with colchicine to prohibit the isolation of chromosomes and induce tetraploid mutants. Results Tetraploids were achieved successfully. The most efficient treatment for inducing tetraploid was soaking the tiny buds in 1?103 mg/L colchicine solution for 24 h, the inducing rate could be up to 35. 2%. The induced tetraploids exhibited notable difference with common wild type (diploids) in morphology, physiology, and microscopic structure. ConclusionThe tetraploid plants show the advantages of gigantic size and vigorous growth. Thus, the established technique system to induce tetraploid from tissue cultured callus would provide an efficient alternative pathway for medicinal plants of Dioscorea L. breeding.

FURTHER OBSERVATION ON DYNAMICS OF IN VITRO GRANULOMATOUS RESPONSES SURROUNDING SCHISTOSOME EGGS / 中国血吸虫病防治杂志

Objective To further observe the morphologic changes of in vitro model of granulomatous responses around Schistosoma japonica eggs and determine the relationship of reaction inten-suy to infection stage of those schistosome-infected mice from which the splenocytes were obtained for in vitro experiments.Methods The spleen cells isolated from schistosome-infected mice were incubated and dry schistosome eggs were added into those cultures. These splenocytes responses surrounding eggs were observed and counted under the microscope.Results The splenocytes from infected mice showed obvious adherent responses to the dry eggs. Adhering cells were,at first,granulocytes,then replaced by the macrophages. Around the eggs proliferating lymphocytes,later fibroblasts, were found. The reaction index (RI) of the spleen cells from infected mice were significantly higher (P

FURTHER STUDIES ON THE DIAGNOSIS OF SCHISTOSOMJASIS JAPONICA BY INDIRECT FLUORESCENT ANTIBODY TEST AND IMMUNOENZYMATIC STAINING TECHNIQUE / 中国血吸虫病防治杂志

Both indirect fluorescent antibody test (IFAT) and immunoenzymatic staining technique(IEST) were used as methods for the comparable detections of 200 cases of patients with schistosomiasis japonica and 100 healthy individuals. The average positive rate in schistosomiasis cases is 99% with both tests, while the false positive rate in healthy individuals is 5% with the former and 3% with the latter. When 322 cases of other parasitic infections and patients with medical diseases were tested by IFAT and IEST, cross-reaction was observed only in clon-orchiasis, fasciolopsiasis and paragonimiasis, 4-6% with IFAT and 0-4% with IEST respectively. 87.6-93.7% showed negative reaction to both tests in schistosomiasis patients during different stages after treatment. It is considered that IFAT and IEST are effective means of serological diagnosis of schistosomiasis japonica, while IEST can be applied suitably in tbe fields for practical purposes.

ANALYSIS ON EIGHT ANTIGEN PREPARATIONS OF SCHISTOSOMA JAPONICUM AND THEIR REACTIVITY WITH SERA FROM PATIENTS OF DIFFERENT STAGES / 中国血吸虫病防治杂志

Eight antigen preparations of schistosama japonicum. i. e. freezethawed antigens (FFT, F-MFT), saline-extract antigens (FE. F-ME, ME) SEA and AWA, were separated by SDS-PAGE. The results showed different fractional proteins among the eight antigens. These above mentioned antigens were used to detect sera from patients of different stages of schis-tosomiasis by means of ELIB. It demonstrated that the defined .fractional proteins below 60KD could be recognized by sera from acute schistosomiasis, the defined fractional proteins over 60KD recognized preferentially by sera from advanced schistosomiasis, while the fractional proteins of 87KD, 70KD, 60KD, 31KD and 18KD of S. japanicum antigens could be recognized by almost all sera from patients of different stages.

DIAGNOSTIC VALUE AND FACTORS EFFECTING OF IMMUNOENZYMATIC STAINING TEST(IEST) FOR SCHISTOSOMIASIS JAPONICA / 中国血吸虫病防治杂志

The most suitable antigenic frozen sections are the sections of infected mice liver and adult worm of Schistosoma japonicum at 7th week and 6th week after infection respectively; the most suitable thickness of section is 7?m. The antigenicity of liver tissue-egg frozen section(TEFS) is markedly higher than that of adult worm frozen sectin(AWFS) and of liver tissue-egg paraffin embedded section.TEFS, as antigen, is considered to be of high sensitivity(96.7%), high specificity (98.7%) and lower cross reaction rates(0~3.3%) in IEST for diagnosis of schistosomiasis. The sensitivity of TEFS-IEST ig markedly higher than that of AWFS-IEST and that of COPT. The main advantages of TEFS-IEST are lower cost of the antigen, high sensitivity and rapid yield of results.

STUDIES ON THE EFFECT OF IMMUNOENHANCING REAGENT “425” ON EGG GRANULOMA FORMATION IN MICE INFECTED WITH SCHISTOSOMA JAPONICUM / 中国寄生虫学与寄生虫病杂志

Fourteen-34 days after Schistosoma japonzcum-infected mice were injected intraperitoneally with immunoenhancing reagent,Chinese angelica root extract ("425"),the specific antibody (IgG) levels were significantly higher in Group Ⅲ (362.67?162.21-480.00?289.45) than the control group (66.67?39.68-245.33?101.56) in experiment one,and in Gronp Ⅱ(488.00?320.38-768.00?267.38) than the control group (256.00?189.07-394.67?141.06) in experiment two.The egg granulomatous formation were markedly diminished in the injected mice.The ratios of granulomas vs.eggs' mean diameters of Group in of mice were 5.24?1.04-3.95?0.77 and those of the control group were 6.59?1.19-5.29?0.94 in experiment one,those of the group Ⅱ were 3.75?0.71-4.15?0.73 and those of the control group were 4.94?0.81-5.36?0.97 in experiment two.Meantime the egg antigen levels in the injected mice might be lower.This study shows that the control of immunomodulation of granulomatous formation in the hosts injected with immunoenhance reagent "425" can be induced.

COMPARISON OF IMMUNOENZYMATIC STAINING TECHNIQUE (IEST), DOUBLE GLUING STRIP CIRCUMOVAL PRECIPITIN TEST(DGS-COPT) AND CONVENTIONAL CIRCUMOVAL PRECIPITIN TEST(CV-COPT) FOR DIAGNOSIS OF SCHISTOSOMIASIS JAPONICA / 中国寄生虫学与寄生虫病杂志

IEST, DGS-COPT and CV-COPT using lyophilized ova of schistosoma japonicum were performed on sera from 120 cases of schistosomiasis japonica, 120 cases of schis-tosomiasis japonica 3-8 years after being cured with praziquantel and 120 healthy individuals by single-blind method. The sensitivity and specificity of IEST was 91.7% and 95.8% respectively which were significantly higher than that of both DGS-COPT and CV-COPT. The negative conversion rate of cured patients was 70.8% with IEST, 80.8% with DGS-COPT and 81.7% with CV-COPT. The results showed that IEST has higher diagnostic value for schistosomiasis than both COPT. DGS-COPT has the same diagnostic value as CV-COPT, however, it was easy to perform and time-saving, thus it might be applied in the fields for practical purposes.

DETECTION OF ENZYME DISSOCIATED SCIC FROM SERA OFSCHISTOSOMIASIS JAPONICA BY TWO-DIRECTION COUNTER.IMMUNOELECTROPHORESIS / 中国寄生虫学与寄生虫病杂志

A new method of two-direction counter-immunoelectrophoresis (TD-CIEP and TD-ELACIEP) for detection of specific antigen and antibody of enzyme-dissociated schisto-some circulating immune complexes (SCIC) in infected rabbits and advanced schistosomia-sis cases was reported. Results showed that the detection rate was markedly increased in the enzyme-dissociated sera as compared with that of non-dissociated sera. The levels of antigen and antibodies were found to be correlated to the period and intensity of infection. The detected antigen was solely originated from the adult worm. Comparison of sensitivity of both methods showed that TD-ELACIEP was much more sensitive than TD-CIEP either for detection of antigen or antibodies. Of 54 sera of advanced schistosomiasis cases the detection rate of schistosome antigen or antibodies by TD-ELACIEP in enzyme-dissociated and non-dissociated serum samples was 50 and 25.9%, respectively. Only 9 cases( 16.7%) were COP positive with COP rate

STUDIES ON THE DIAGNOSIS OF HUMAN FILARIASIS BY INDIRECT FLOURESCENT ANTIBODY TEST(IFAT) AND IMMUNOENZYMATIC STAINING TECHNIQUE(IEST) WITH HOMOLOGOUS AND HETEROLOGOUS ADULT FILARIAL ANTIGENS / 中国寄生虫学与寄生虫病杂志

This article deals with the diagnostic values of IFAT and IEST in human filariasis with both frozen sections of Brugia malayi and Setaria cervi adult worms as antigens. The average positive and the false positive rates of IFAT with two antigens were 84.9-97.1%(45/53-102/105)and 2.9-9.7%(1/35-3/31) respectively, while no cross-reaction was observed in subjects infected with ascaris or hookworm. The average positive and the false positive rates of IEST with two antigens were 94.3-94.9% (99/105-56/59) and 0-2.9% (0/35-l/35) respectively. Both IFAT and IEST with two antigens for the diagnosis of human filariasis were considered to be of higher sensitivity and specificity, frozen sections of adult Setaria cervi being more economical and effective.