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1.
Parenteral & Enteral Nutrition ; (6): 10-12, 2017.
Artigo em Chinês | WPRIM | ID: wpr-509894

RESUMO

Objective:To evaluate the nutritional status before and after chemotherapy in patients with colon cancer by measuring energy metabolism and body composition.Methods:Fifty-one patients with colon cancer were included and were investigated using self-control method (before and after chemotherapy).Resting energy expenditure (REE) was determined by bed metabolic instrument,and body composition was measured by body composition analyzer.Results:For all patients,the REE on the 2nd day after chemotherapy was decreased significantly compared with that before chemotherapy (P < 0.05).After chemotherapy,body fat percentage and body moisture percentage were increased significantly (P < 0.05),and lean body mass percentage was decreased significantly (P < 0.05).Conclusion:After chemotherapy,the REE and lean body mass percentage of the patients with colon cancer were decreased,and body fat percentage and body moisture percentage were increased.

2.
Chinese Journal of Biotechnology ; (12): 35-41, 2010.
Artigo em Chinês | WPRIM | ID: wpr-336264

RESUMO

In previous study we isolated a gram-positive bacterial strain, designated Niu-O16, from bovine rumen gastric juice. The growing cells of bacterial strain Niu-O16 is capable of biotransforming isoflavone daidzein into dihydrodaidzein efficiently under anaerobic conditions. In this study we investigated the optimal bioconversion conditions for the resting cells of bacterial strain Niu-O16 to convert daidzein into dihydrodaidzein. Single factor test showed that the optimal conditions for the initial pH of phosphate buffer, the concentration of the resting cell and the concentration of the substrate daidzein were 6.0-8.0, 32-64 mg/mL (wet weight) and 0.8-1.2 mmol/L, respectively. Orthogonal experiments were used to determine the optimal combination of the resting cell concentration, substrate concentration and biotransformation time. The results showed that the optimal combination included resting cell concentration 32 mg/mL, substrate concentration 0.8 mmol/L and the biotransformation time 24 h. Furthermore, the biotransformation kinetics under optimal conditions were studied, under which conditions the highest bioconversion rate was 63.9% in the resting cell system. The results might provide information for resting cell biotransforming of anaerobes as well as its industrial application.


Assuntos
Animais , Bovinos , Anaerobiose , Biotransformação , Técnicas de Cultura , Métodos , Suco Gástrico , Microbiologia , Bactérias Gram-Positivas , Fisiologia , Isoflavonas , Química , Metabolismo , Cinética , Rúmen , Microbiologia
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