Hyperbaric oxygen therapy can effectively treat diabetic foot wounds / 中华物理医学与康复杂志

Objective:To observe the effect of supplementing vacuum sealing drainage with hyperbaric oxygen in the short term treatment of diabetic foot ulcers.Methods:A total of 156 persons diagnosed with diabetic foot ulcers were randomly divided into a control group and a treatment group, each of 78. Both groups received life guidance and active treatment to lower blood sugar and lipids, as well as anti-infection treatment guided by bacterial cultures. Both groups′ wounds were debrided. The wound was then covered with foam, sealed, and negative pressure of -75 to -100mmHg was applied during 1 week of drainage. Two courses of this treatment were applied. In addition, the treatment group received hyperbaric oxygen daily during the two weeks. The exposure pressure was incrased to 0.25MPa over 15min with 100% oxygen. That was inhaled in two 30min sessions with a 10min interval. The pressure then decompressed at a constant rate for 25 minutes. Wound healing, hemorheology, wound granulation tissue staining and any changes in TGF-β1 were observed before as well as after 7 and 14 days of the treatment.Results:The average wound size and symptom score of both groups had improved significantly after the treatment, with the largest effect in the treatment group during the first week. Both groups′ hemorheology had improved significantly after one week, but the treatment group′s improvement was greater. After 2 weeks, however, there was no significant difference in the average hemorheologic indicators for either group compared with before the treatment. Hematoxylin-eosin staining of the wound tissues showed that there were many inflamed cells before the treatment, with relatively little fresh granulation tissue or new blood vessels. After one week of treatment much new granulation tissue was observed under the microscope in both groups, with no significant difference between them. One week later, there was still much granulation tissue in the control group, but slightly less in the treatment group. The ave-rage post-treatment TGF-β1 protein levels in the wound tissues of both groups were significantly higher than before the treatment, but after two weeks the average TGF-β1 protein level had decreased significantly in the treatment group compared with the control group.Conclusions:One week of hyperbaric oxygen treatment can effectively improve the hemorheology of persons with diabetic foot ulcers, promote the proliferation of granulation tissue and fibroblasts, and increase the level of TGF-β1 protein in the wound tissues. However, the effects of hyperbaric oxygen treatment weaken gradually with time.

Meta-analysis of transperitoneal and retroperitoneal laparoscopic decortication in the treatment of simple renal cysts / 国际外科学杂志

Objective:To evaluate the clinical effects of transperitoneal and retroperitoneal laparoscopic decortication in the treatment of simple renal cysts.Methods:PubMed, EMBASE, Cochrane Library, CNKI, VIP and WanFang databases were searched, All studies about comparing transperitoneal and retroperitoneal laparoscopic decortication for simple renal cysts were included. The evaluation indicators included operative time, intraoperative blood loss, postoperative drainage time, postoperative hospital stay, and complication rate. Statistical analysis of the data using RevMan 5.3 software.Results:A total of 14 control studies were included, 409 cases in the transperitoneal group and 452 cases in the retroperitoneal group. Meta-analysis demonstrated that compared with the transperitoneal group, the retroperitoneal group had shorter operation time ( MD=8.81, 95% CI: 3.79-13.82, P<0.01), less intraoperative blood loss ( MD=4.89, 95% CI: 1.40-8.38, P<0.01), and shorter postoperative hospital stay ( MD=0.60, 95% CI: 0.24-0.96, P<0.01). However, there were not significant differences between the two approaches in postoperative drainage time ( MD=-0.12, 95% CI: -0.26-0.03, P=0.13) and complication rate ( OR=1.47, 95% CI: 0.48-4.47, P=0.05). Conclusions:Compared with the transperitoneal, the retroperitoneal laparoscopic decortication has the advantages of short operation time, less intraoperative blood loss and short postoperative hospital stay. Therefore, retroperitoneal laparoscopic decortication is expected to be the first choice for the treatment of simple renal cysts.

Association of mental health,job burnout and mental fatigue in mental workers / 中国职业医学

OBJECTIVE: To explore the association of mental health,job burnout and mental fatigue in mental workers.METHODS: A total of 276 mental workers in 11 research institutes in Ningxia were selected as research subjects by convenient sampling method. The questionnaire survey was carried out using the scales of Self-Reporting Inventory( SCL-90),Maslach Burnout Inventory-General Survey and Mental Fatigue subscale of Fatigue Scale-14 to investigate the workers' mental health,job burnout and mental fatigue. RESULTS: The scores of SCL-90 and job burnout in mental workers were( 142. 4 ± 42. 2) and( 41. 8 ± 10. 5). The medium( P25,P75) mental fatigue total score was [3. 0( 2. 0,4. 0) ].The correlation analysis showed that the total score of SCL-90 was positively correlated with that of mental fatigue and job burnout( P < 0. 01). The job burnout was the intermediate variable between mental fatigue and mental health( 43. 4% of the total effect). The dimension of low sense of accomplishment was the regulating variable. CONCLUSION: Job burnout plays an intermediate role between mental fatigue and mental health in mental workers. The low sense of accomplishment plays a regulatory role.

Dexmedetomidine combined with remifentanil used for sedation and analgesia in ICU patients with hypertensive cerebral hemorrhage / 中华急诊医学杂志

Objective To explore the administration of Dexmedetomidine combined with remifentanil for sedation and analgesia of ICU patients with hypertensive cerebral hemorrhage after operation.Methods A total of 60 patients with hypertensive cerebral hemorrhage treated with hematoma removal under craniotomy were selected from May 2013 to June 2015.The patients were randomly (random number) divided into the Dexmedetomidine combined with remifentanil group (D + R, n =30), and Midazolam combined with remifentanil group (M + R, n =30).The blood pressure, respiration rate, oxygen saturation, heart rate, ICP (intracranial pressure), Ramsay sedation scores, and IL-1, and TNF-α levels were recorded after sedation and analgesia in ICU, and 6 h, 24 h, 48 h after operation (T0-T3).Results Compared with M +R group, the MAP, RR, HR, ICP, IL-1β, TNF-α, rate of reoperation for check bleeding, and mortality were significantly decreased in D + R group (P ＜ 0.05), and Ramsay sedation score was significantly increased at the same time (P ＜ 0.05) without excessive sedation and analgesia noticed.Conclusions Dexmedetomidine combined with remifentanil exhibits significant benefit in many respects including control of great fluctuations of blood pressure and intracranial pressure after craniotomy, reduce the production and release of inflammatory mediators, reduce the occurrence of rebleeding after operation.It shows good controllability and safety, it is an optimal method producing sedation and analgesia in ICU patients with hypertensive cerebral hemorrhage after operation.

Adipose-derived stem cells transfected with adenovirus carrying bone morphogenetic protein 14 for repair of articular cartilage injury / 中国组织工程研究

BACKGROUND:The articular cartilage has weak self-repair ability, mainly due to its lack of trophoblast cels in blood vessels and slow cel metabolism. Current treatment methods cannot restore the original function of the cartilage tissue, and cartilage tissue engineering in recent years has garnered increasing attention. OBJECTIVE:To observe the effect of adipose-derived stem cels transfected with bone morphogenetic protein 14 combined with type I colagen sponge scaffold on the repair of articular cartilage injury in the knee of rabbits. METHODS: Adipose-derived stem cels were isolated and cultured from rabbit subcutaneous adipose tissue, and transfected with Ad-CMV-BMP-14-IRES-hrGFP-1. Type I colagen sponge scaffold with the transfected adipose-derived stem cels was used to repair articular cartilage injury in the knee of rabbits. Twelve weeks after operation, the articular tissue was taken for gross assessment and histological evaluation. RESULTS AND CONCLUSION: The expressions of bone morphogenetic protein 14, type II colagen and Sox-9 were higher in cels transfected with bone morphogenetic protein 14 than untransfected ones. At 12 weeks after operation, adipose-derived stem cels transfected with bone morphogenetic protein 14 combined with type I colagen sponge scaffold had good repair effect on articular cartilage injuries, and the injured cartilage tissues were smooth and had good texture, color and integration junction; adipose-derived stem cels combined with type I colagen sponge scaffold could partialy repair the injured cartilage tissues that had similar color and texture to normal tissues, and there was a remarkable boundary between the repaired tissue and normal cartilage tissue;simple type I colagen sponge scaffold was almost colapsed, and no hyaline cartilage tissue formed. These findings indicate that transfection of bone morphogenetic protein 14 can strengthen the ability of adipose-derived stem cels dramaticaly to repair cartilage injuries.

Research on growth state and physiological changes of Tianquan Bergenia purpurascens under different shade conditions / 中国中药杂志

<p><b>OBJECTIVE</b>To study the growth index and physiological index of Tianquan Bergenia purpurascens under different shade conditions (100%, 80%, 60%, 40%, 20%), and investigate its adaptation mechanism to shade environment so that it can provide theoretical basis for cultivation and production.</p><p><b>METHOD</b>The growth index such as the length, the width and the number of new leaves, and leaf area, fresh weight, dry weight and specific leaf weight of the samples which were collected from the beginning of March to late May under different shade conditions were measured. The physiological index including the contents of photosynthetic pigments, MDA, soluble sugar, soluble protein, proline and the activity of SOD were measured all together.</p><p><b>RESULT</b>The growth and morphological index under light intensity 100%-60% were higher than those under light intensity 40%-20%. And except specific leaf weight, the growth and morphological index were significantly higher under light intensity 60% than those under the other shade conditions; The contents of Chla, Chlb, Chl (a + b) and Car under light intensity 100%-60% were higher than those under light intensity 40%-20%, while Chla/b was higher under light intensity 40%-20%; The activity of SOD, soluble sugar, soluble protein and proline contents were higher under light intensity 100%-60%, while MDA content was higher under light intensity 40%-20%. Chl(a+b) content, soluble sugar content and activity of SOD were the highest under light intensity 60%, but MDA content was the lowest.</p><p><b>CONCLUSION</b>Sixty percent irradiance treatment was the most favorable to the growth of Tianquan B. purpurascens; 100%-80% irradiance treatments were favorable to its growth; 40%-20% irradiance treatment was negative for its growth.</p>

Research on growth state and physiological changes of Epimedium wushanense under different shade conditions / 中国中药杂志

<p><b>OBJECTIVE</b>To study the growth state and physiological changes of Epimedium wushanense under different light conditions (100%, 80%, 60%, 40%, 20%), and investigate its adaptation mechanism to shade environment so that it can provide theoretical basis for cultivation and production.</p><p><b>METHOD</b>The growth index, the content of photosynthetic pigments, proline content, MDA content, soluble sugar content, soluble protein content and antioxidant enzymy activity of E. wushanense under different shade conditions were measured.</p><p><b>RESULT</b>Except for length/width, the other form index of E. wushanense changed significantly. The E. wushanense grew well between light intensity 80%-40%, besides, the biomass above the earth was relatively high. The chlorophyll a, chlorophyll b, total chlorophyll content and carotenoids cotent were enhanced after shaded, the values of chlorophyll a\chlorophyll b increased between light intensity 100% -60% and reduced between light intensity 60% -20%. The proline and MDA content decreased after shading, the soluble sugar and soluble protein content increased respectively between light intensity 100% -60%, 100% -40% and reduced respectively between light intensity 60% -20%, 40% -20%. The SOD activity, CAT activity decreased after shading, and the POD activity, APX activity reduced between light intensity 60% -40%, raised under 20% treatment.</p><p><b>CONCLUSION</b>80% 40% irradiance treatment was favorable to the growth of E. wushanense.</p>

Expression and clinical significance of E2F-3 and pRb in human prostate cancer / 第三军医大学学报

Objective To study the expression of E2F-3 and pRb in primary prostate cancer(PCa) and its clinical significance.Methods The expression of E2F-3 protein and pRb was detected in 49 PCa,20 benign prostatic hyperplasia(BPH),10 normal prostate tissues(NP) by EliVision~(TM) plus immunohistochemical staining.Results The positive expression rate of E2F-3 in PCa,BPH,NP was 63.27%(31/49),20%((4/20)) and 10%(1/10),respectively.The expression level of E2F-3 in PCa was significantly higher than that in BPH(P

Expression and clinical significance of Rb gene and MCM2 in prostate cancer / 重庆医科大学学报

Objective: To study the expression of retinoblastoma susceptibility gene(Rb) and minichromosome maintenance protein 2(MCM2) in primary prostate cancer(PCa) and its clinical significance.Methods: The expression of MCM2 protein and pRb were detected in 49 cases of PCa,20 cases of benign prostate hyperplasia(BPH),10 cases of normal prostate tissues(NP) by EliVisionTM plus immunohistochemical staining.Results: The positive expression rate of pRb in PCa,BPH,NP were 44.90%,80% and 90% respectively.The expression level of pRb in PCa was significantly lower than that in BPH(P