RESUMO
Objective To examine whether sleep problems are related to both emotional and behavioral problems in children aged 3-6 years.Methods A large cross-sectional study was conducted in Anqing,Wuhu,Tongling and Yangzhou from March to June 2015.A total of 8 900 preschool aged children were included.Sleep problems were obtained by using adapted BISQ completed by the parents or the people who took care of children.Emotional and behavioral problems of the children were accessed by using Strengths and Difficulties Questionnaire (SDQ),and multivariate logistic regression model was used for statistical analyses.Results The detected rates of emotional symptoms,conduct problems,hyperactivity problems,peer problems,total difficulties and prosocial behavior in preschool aged children were 9.0％,13.9％,18.9％,25.5％,13.6％ and 16.2％ respectively.All the detected rates were higher in boys than in girls except the higher rate of emotional symptoms.The proportions of children with high sleep quality,moderate sleep quality and poor or worse sleep quality were 3.9％,52.9％ and 43.2％ respectively.After controlling the confounding factors of demographic variables,including gender,age,delivery mode,birth weight,birth height and patent' s educational level,multinomial logistic regression analysis showed that the risk of emotional symptoms,conduct problems,hyperactivity problems,peer problems,total difficulties and prosocial behavior in children with longer sleep duration was lower than that in children with shorter sleep duration,the ORs were 0.86 (95％CI:0.77-0.95),0.85 (95％CI:0.78-0.93),0.85 (95％CI:0.79-0.92),0.87(95％CI:0.81-0.93),0.83 (95％CI:0.76-0.91) and 0.82 (95％CI:0.76-0.89) respectively.Compared with the children with good sleep quality,the risk of emotional symptoms,conduct problems,hyperactivity problems,peer problems,total difficulties and prosocial behavior were higher in children with poor or worse sleep quality,the ORs were 3.26 (95％CI:2.40-4.42),2.86 (95％CI:2.16-3.78),2.60 (95％ CI:2.00-3.38),1.96 (95％ CI:1.52-2.54),4.02 (95％ CI:3.06-5.27) and 2.56 (95％ CI:1.96-3.35) respectively.Conclusion There was a negative impact of shorter sleep and poor or worse sleep on emotional and behavioral problems of preschool aged children.
RESUMO
Objective To examine whether sleep problems are related to both emotional and behavioral problems in children aged 3-6 years.Methods A large cross-sectional study was conducted in Anqing,Wuhu,Tongling and Yangzhou from March to June 2015.A total of 8 900 preschool aged children were included.Sleep problems were obtained by using adapted BISQ completed by the parents or the people who took care of children.Emotional and behavioral problems of the children were accessed by using Strengths and Difficulties Questionnaire (SDQ),and multivariate logistic regression model was used for statistical analyses.Results The detected rates of emotional symptoms,conduct problems,hyperactivity problems,peer problems,total difficulties and prosocial behavior in preschool aged children were 9.0％,13.9％,18.9％,25.5％,13.6％ and 16.2％ respectively.All the detected rates were higher in boys than in girls except the higher rate of emotional symptoms.The proportions of children with high sleep quality,moderate sleep quality and poor or worse sleep quality were 3.9％,52.9％ and 43.2％ respectively.After controlling the confounding factors of demographic variables,including gender,age,delivery mode,birth weight,birth height and patent' s educational level,multinomial logistic regression analysis showed that the risk of emotional symptoms,conduct problems,hyperactivity problems,peer problems,total difficulties and prosocial behavior in children with longer sleep duration was lower than that in children with shorter sleep duration,the ORs were 0.86 (95％CI:0.77-0.95),0.85 (95％CI:0.78-0.93),0.85 (95％CI:0.79-0.92),0.87(95％CI:0.81-0.93),0.83 (95％CI:0.76-0.91) and 0.82 (95％CI:0.76-0.89) respectively.Compared with the children with good sleep quality,the risk of emotional symptoms,conduct problems,hyperactivity problems,peer problems,total difficulties and prosocial behavior were higher in children with poor or worse sleep quality,the ORs were 3.26 (95％CI:2.40-4.42),2.86 (95％CI:2.16-3.78),2.60 (95％ CI:2.00-3.38),1.96 (95％ CI:1.52-2.54),4.02 (95％ CI:3.06-5.27) and 2.56 (95％ CI:1.96-3.35) respectively.Conclusion There was a negative impact of shorter sleep and poor or worse sleep on emotional and behavioral problems of preschool aged children.
RESUMO
Objective The study is to investigate the role of miR-328 in endothelial mesenchymal transition (EMT)induced by high glucose in human umbilical vein endothelial cells (HUVECs)and its signaling mechanism.Methods HUVECs were cultured in high glucose environment to induce EMT;The recombinant lentiviruses were created by miR-328 and antagomiR- 328 transfection of HUVECs.The experiment was divided into seven groups: normal glucose;mannitol group;high glucose;miR-328;miR-328 virus negative control;high glucose + U0126;miR-328 + U0126.Double immunofluorescent staining was used to determine expression of EMT markers;Changes in miR-328 expression is examined by RT-qPCR;The expressions of type Ⅰ/Ⅲ collagen,p-MEK1/2 and p-ERK1/2 are examined by Western blot.Results 1)HUVECs showed positive staining for CD31 and α-SMA in high glucose group.2)The expression of miR-328 was up-regulated(P<0.05)in HUVECs treated by high glucose or miR-328.Compared with high glucose group or miR-328 group,miR-328 expression was less pronounced aftertreatment with U0126.3)The expressions of type Ⅰ/Ⅲ collagen increased in HUVECs treated by high glucose or miR-328(P<0.05) Compared with high glucose group or miR-328 group,typeⅠ/Ⅲ collagen expressions were less pronounced after treatment with U0126.4)The expressions of p-MEK1/2 and p-ERK1/2 were increased in HUVECs treated by high glucose or miR-328 in comparison to the control group (P<0.05);a lower expression of p-MEK1/2 and p-ERK1/2 were observed in U0126 group than in high glucose group or miR-328 group.Conclusions The phenomenon of EMT in HUVECs is induced by high glucose with increased expression of miR-328;overexpression of miR-328 induced EMT in HUVECs;miR-328 induced EMT is related with MEK1/2-ERK1/2 signaling pathway.