Relationship between uncertainty stress and occupational well-being of medical staff / 中国实用护理杂志

Objective:To understand the status of uncertainty stress and occupational well-being of medical staff in Zhejiang Province, and to analysis the relationship between them, in the context of a pandemic of novel coronavirus pneumonia (corona virus disease 2019, COVID-19).Methods:From May to June 2021, 1 028 medical staff were selected from 8 Class Ⅲ hospitals in Zhejiang Province by convenience sampling. The cross-sectional survey was conducted with the General Information Questionnaire, the Uncertainty Stress Scale and the Occupational Well-being Scale. Pearson method was used to analyze the correlation between uncertain psychological stress and occupational well-being of medical staff, and multiple linear regression was used to analyze the influencing factors of occupational well-being of medical staff.Results:The total score of uncertainty stress of 1028 medical staff was (25.62±8.92), and the total score of occupational well-being was (75.03±14.68) at a moderate level, including 48 (4.7%) participants with a high level of occupational well-being. Uncertainty stress of medical staff was negatively correlated with occupational well-being ( r=-0.46, P<0.01). Multiple regression analysis showed that working years, hospital class, interests, leisure time, personality traits, status of self-reported health and levels of uncertainty stress had statistically significant influence on occupational well-being ( t=-2.30-10.28, all P<0.05). Conclusions:The occupational well-being of medical staff in Zhejiang Province needs to be improved. Health administrative departments should formulate targeted intervention measures to enhance the ability of medical staff to cope with uncertainty stress, so as to improve the occupational well-being of medical staff.

Analysis of transcriptional factors expression profile during dedifferentiation of liver cell cultured in vitro / 生物工程学报

Primary hepatocytes are widely used in drug metabolism and toxicity assessment. As the culture of primary hepatocytes in vitro is a process of dedifferentiation, hepatocytes lose normal metabolic detoxification function gradually. The mechanism of hepatocyte dedifferentiation has been not clear so far. TFs play an important role in the dedifferentiation and non-parenchymal cells can maintain the function of hepatocytes in vitro. However, the current methods cannot be used in effective identification and quantitative analysis of a large number of TFs. In this paper, the mo-culture system (only primary hepatocytes) and co-culture system (primary hepatocytes and non-parenchymal cells) were established. The cells were cultured for 24 h, 48 h, 72 h as monolayer. The changes of TFs during the culture were obtained by TOT (Transcription factor response elements on tip) transcription factor enrichment method and mass spectrometry. A total of 219 TFs were identified in three individual replicates. The result revealed that up-regulated TFs were enriched in cell proliferation, death and immune response pathways, and down-regulated TFs were involved in metabolism pathway. The establishment of such culture-TFs identification system is of great significance to reveal the mechanism of primary hepatocyte dedifferentiation and crosstalk between hepatocytes and non-parenchymal cells.

Research progress of optical coherence tomography combined with microperimetry in diabetic macular edema / 中华眼底病杂志

Diabetic macular edema is the major cause of vision impairment in patients with nonproliferative diabetic retinopathy.Thickness and pathological alterations in each retina layer of diabetic macular edema (DME) patients can be performed by optical coherence tomography (OCT) device.And retinal light sensitivity at specific retinal point and fixation state can be detected by microperimetry qualitatively and quantitatively.Moreover,OCT can discover pathological anatomical changes in the retina of DME patients,thus facilitating the interpretation of the structure-function relationship in DME with combination of microperimetry results.At present,there are various therapies for DME patients,and the primary method in evaluating therapeutic efficacy is to compare the pathological changes in the retina before and after treatment by OCT.Besides,microperimetry can provide information in visual function restoration.The combined application of OCT and microperimetry has broad prospects in the diagnosis and treatment of DME patients.

Therapeutic Observation of Acupuncture for Acute Cerebral Infarction Coupled with Obstructive Sleep Apnea-hypopnea Syndrome / 上海针灸杂志

Objective To observe the clinical efficacy of acupuncture in treating acute cerebral infarction coupled with obstructive sleep apnea-hypopnea syndrome (OSAHS).Method Totally 126 patients with acute cerebral infarction coupled with OSAHS were randomized into a treatment group and a control group, 63 cases in each group. The control group was intervened by conventional comprehensive treatment, while the treatment group was additionally intervened by acupuncture treatment. The OSAHS indexes were observed before and after the treatment, and the clinical efficacies were compared between the two groups. Result The OSAHS scores (AHI, minimum SaO2, and ESS score) were significantly changed in both groups after the intervention (P<0.01,P<0.05). The OSAHS scores of the treatment group were significantly different from that of the control group after the intervention (P<0.05). The total effective rate was 93.7% in the treatment group versus 82.5% in the control group, and the difference was statistically significant (P<0.05).Conclusion Acupuncture is an effective approach in treating acute cerebral infarction coupled with OSAHS.

Therapeutic Observation of Acupuncture for Relapsed Migraine / 上海针灸杂志

Objective To observe the clinical efficacy of acupuncture in treating relapsed migraine. Method Totally 126 patients with relapsed migraine were randomized into a treatment group and a control group, 63 in each group. The treatment group was intervened by acupuncture, while the control group was by medication. The onset time of remission was observed, and the clinical efficacy was compared. Result The onset time of remission was (0.8±1.7)h in the treatment group versus (1.3±1.8)h in the control group, and the difference was statistically significant (P0.05). Conclusion Acupuncture is an effective method in treating relapsed migraine.

Analysis and Deployment of Network Security in Hospital Information System Construction / 医疗卫生装备

Objective To analyze and deploy in the hospital network security for guarantee hospital network and information of safety, and ensure stability operation in the hospital management information system. Methods The network environment was based on the original hospital, which was added to measures about the isolation for network security, the prevention of computer viruses and firewall, data backup and recovery, physical safety management and so on. Results After the comparatively perfect measure of network security was deployed, the security of hospital information and data was effectively protected, and the security threat was solved from the inner and outer network. Conclusion The deployment of network security prevention system can better give fully play to important function on which management information system can efficiently operate in whole hospital.

Determination of astragaloside Ⅳ and salidroside in Zhenqi Fuzheng Capsule / 中成药

AIM: To establish the method of determining astragaloside Ⅳ and salidroside in Zhenqi Fuzheng Capsule(Fructus Ligustri Lucidi,Radix Astragali,etc.). METHODS: Astragaloside Ⅳ was determined by HPLC-ELSD on Inertsil ODS-3 C_(18) column(250 mm?4.6 mm,5 ?m) using a mobile phase of acetonitrile-water(37∶63).The flow rate was 1.0 mL/min and detector parameters were set as follows: drift tube temprature was at (106 ?C);carrier gas(air) flow rate was 2.7 L/min.Salidroside was determined by RP-HPLC on the same column using a mobile phase composed of acetonitrile-water with gradient elution.The detection wavelength was at 278 nm and the flow rate was 1.0 mL/min. RESULTS: Calibration curve of astragaloside Ⅳ was linear between 0.76 ?g and 3.8 ?g under the chromatographic condition,R=0.999 5.The average recovery of astragaloside Ⅳ was(98.4%).RSD was 0.9%(n=6).And the calibration curve of Salidroside was linear between 0.4 ?g and 4 ?g,R=0.999 9.The average recovery of salidroside was 100.7%.RSD was 2.1%(n=5). CONCLUSION: These methods were simple,accurate and sensitive,so it can be used for the quality control of Zhenqi Fuzheng Capsule.