RESUMO
<p><b>OBJECTIVE</b>To study the morphological characteristics and immunophenotype of highly cellular leiomyoma (HCL) of uterus, compared with that of uterine endometrial stromal tumors (EST).</p><p><b>METHODS</b>HE and immuno-stained sections EnVision method from 20 cases of HCL, 21 cases of EST and 1 case of stromomyoma were reviewed. Monoclonal antibodies against h-caldesmon, calponin, CD10, desmin and smooth muscle actin (SMA) were used for immunohistochemistry studies.</p><p><b>RESULTS</b>On microscopic examination, HCL were densely cellular and composed of cells that ranged from spindle-shaped to round with scanty cytoplasm. A focal fascicular pattern was present in all cases. Blood vessels with large, thick muscular walls were a conspicuous feature of the majority of tumors. Cleft-like spaces were present in 9 tumors and 15 cases exhibited irregular focal extensions into the adjacent myometrium. ESTs were composed of cells that resembled endometrial stromal cells of proliferative endometrium. These cases included a significant component of delicate blood vessels similar to spiral arterioles. All 20 low grade endometrial stromal sarcoma cases had infiltrative growth to adjacent myometrium. Immunoreactivities of HCL for h-caldesmon, calponin, CD10, Desmin and SMA were 80.0% (16/20), 100% (20/20), 0 (0/20), 95.0% (19/20) and 100% (20/20), respectively, whereas the positive rates of EST were 4.7% (1/21), 23.8% (5/21), 66.7% (14/21), 23.8% (5/21) and 19.0% (4/21), respectively (P = 0.001).</p><p><b>CONCLUSIONS</b>Highly cellular leiomyomas have distinct morphologic features. H-caldesmon, calponin, CD10, desmin and SMA are helpful in the differential diagnosis of HCL and EST.</p>
Assuntos
Adulto , Feminino , Humanos , Pessoa de Meia-Idade , Proteínas de Ligação ao Cálcio , Proteínas de Ligação a Calmodulina , Desmina , Neoplasias do Endométrio , Metabolismo , Patologia , Tumores do Estroma Endometrial , Metabolismo , Patologia , Imuno-Histoquímica , Leiomioma , Metabolismo , Patologia , Proteínas dos Microfilamentos , Neprilisina , Neoplasias Uterinas , Metabolismo , PatologiaRESUMO
Objective To study the clinical, histological and immunohistochemical features of breast hamartomas. Methods The specimens were fixed in 10% formalin and embedded in paraffin. The sections were stained with hematoxylin and eosin for histological diagnosis. SP technique was used in the immunohistochemical study.Results We have 8 cases of breast hamartomas with well defined borders microscopically, histologically composed of ducts, lobules, fibrous stroma, adipose tissue. None of the epithelial elements showed any evidence of atypia. Immunohistochemical analysis showed that there was a variable number of epithelial cells showing a nuclear staining for estrogen receptor(ER) in all cases.Conclution Mammary hamartomas are benign tumor like lesions, histologically dissimillar from other breast tumors. It is affected by hormonal factors. The diagnosis is achieved by combining clinical, radiological and pathologic results.