RESUMO
OBJECTIVE@#To explore the role of SIRT1 in the occurrence of epithelial-mesenchymal transition (EMT) in EC-9706 and Eca-109 cells and the possible mechanism.@*METHODS@#Three chemically synthesized siRNA targeting SIRT1 were transfected into EC-9706 and Eca-109 cells with the non-transfected cells and cells transfected with the negative siRNAs as controls. Real-time PCR and Western blotting were used to detect the expressions of SIRT1, E-cadherin, vimentin, Snail, Twist1 and ZEB in the cells. Transwell invasion assay and wounding healing assay were used to examine the changes in the invasion and metastasis abilities of the cells after transfection.@*RESULTS@#EC-9706 and Eca-109 cells transfected with SIRT1 siRNA1 and SIRT1 siRNA3 showed significantly decreased mRNA and protein expressions of SIRT1 ( < 0.05). Transwell invasion assay and wounding healing assay showed that transfection with SIRT1 siRNA1 and SIRT1 siRNA3 caused significantly lowered invasion and metastasis abilities in EC-9706 and Eca-109 cells ( < 0.05). In EC-9706 and Eca-109 cells transfected with SIRT1 siRNA1 and SIRT1 siRNA3, the expression level of E-cadherin was significantly increased while the expressions of vimentin, Snail and Twist were significantly lowered ( < 0.05).@*CONCLUSIONS@#SIRT1 participates in the invasion and metastasis of EC-9706 and Eca- 109 cells probably by inducing EMT via regulating the expression of Snail.
Assuntos
Humanos , Antígenos CD , Metabolismo , Caderinas , Metabolismo , Linhagem Celular Tumoral , Movimento Celular , Transição Epitelial-Mesenquimal , Fisiologia , Invasividade Neoplásica , Proteínas Nucleares , Metabolismo , RNA Mensageiro , Metabolismo , RNA Interferente Pequeno , Metabolismo , Sirtuína 1 , Genética , Metabolismo , Fatores de Transcrição da Família Snail , Metabolismo , Transfecção , Proteína 1 Relacionada a Twist , Metabolismo , Vimentina , Metabolismo , Homeobox 1 de Ligação a E-box em Dedo de Zinco , MetabolismoRESUMO
<p><b>OBJECTIVE</b>To explore the genetic evolutionary distance between plants by using karyotype parameters identification of medicinal plants.</p><p><b>METHOD</b>The cluster analysis of karyotype resemblance-near coefficient and evolutionary distance was used for 6 Bupleurum species.</p><p><b>RESULT</b>The results showed that there were the biggest karyotype resemblance-near coefficient (0.9920) and the smallest evolutionary distance (D(e) = 0.0080) between B. scorzonerifolium and B. chinense, indicating the closest relationship, and the minimum karyotype resemblance-near coefficient (0.4794) and the maximum evolutionary distance (D(e) = 0.7352) between B. smityii and B. falcatum, indicating the most distant relationship.</p><p><b>CONCLUSION</b>Karyotype was an important parameter for identification of medicinal plants because karyotype was stabilized for species. The genetic distance between in 6 species of Bupleurum species was obtained by karyotype clustering analysis of karyotype resemblance-near coefficient. There was the bigger evolutionary distance between the species which had different chromosome number.</p>
Assuntos
Bupleurum , Classificação , Genética , Análise por Conglomerados , CariótipoRESUMO
The left ventricular systolic function before and after right ventricular pacing was studied in 26 patients by Doppler and M-mode echocardiography. The results showed that there were increases in cardiac output and heart rate, respectively (P