RESUMO
Objective To compare the clinical efficacy and safety of invasive microneedle radiofrequency versus plasma fractional radiofrequency in the treatment of atrophic facial acne scars.Methods A total of 30 patients with atrophic acne scars were enrolled from the Department of Dermatology of Xiangya Hospital affiliated to Central South University between January 2017 and March 2017.By a random number table,every patient randomly received the treatment with invasive microneedle radiofrequency on one half of the face and plasma fractional radiofrequency on the other facial side once every 8-12 weeks for 3 sessions.Before and after each treatment,facial photos were taken,acne scars were scored by the ECCA grading scale (échelle d'évaluation clinique des cicatrices d'acné),and improvement rates of these lesions were evaluated subjectively by doctors.Adverse reactions were recorded after treatment,and the degree of pain and satisfaction was evaluated by the patients themselves.The Germany CK physiological index detector was used to detect the skin moisture content,transepidermal water loss (TEWL) and sebum content.Results All of the 30 patients completed 3 sessions of treatment and follow-up.After the 3 sessions of treatment,the average score on invasive microneedle radiofrequency-treated sides was 3.00 ± 0.91,and 22 patients achieved more than 50％ improvement.However,the average score on plasma fractional radiofrequency-treated sides was 3.57 ± 0.57,and 29 patients achieved more than 50％ improvement.The overall therapeutic effect of plasma fractional radiofrequency was significantly superior tothat of invasive microneedle radiofrequency (t =2.894,P =0.005).For V-shaped and U-shaped scars,there was no significant difference in the overall improvement rate between the invasive microneedle radiofrequency-treated side and plasma fractional radiofrequency-treated side (both P ＞ 0.05).For Mshaped rolling scars,the overall improvement rate was significantly lower on the invasive microneedle radiofrequency-treated side (36.5％ ± 2.1％) than on the plasma fractional radiofrequency-treated side (48.7％ ± 3.4％,P ＜ 0.01).However,the pain score was significantly lower on the invasive microneedle radiofrequency-treated side (5.54 ± 0.57) than on the plasma fractional radiofrequency-treated side (8.07 ± 0.79,P ＜ 0.01).After 3 sessions of treatment,there were no significant differences in the degree of patient satisfaction (x2 =0.10,P ＞ 0.05),skin moisture content (P ＞ 0.05),TEWL (P ＞ 0.05) or sebum content (P ＞ 0.05) between the 2 sides.Adverse reactions such as erythema,exudation and hemorrhage occurred on both of the 2 sides.Conclusions For small V-shaped and U-shaped scars,the plasma fractional radiofrequency and invasive microneedle radiofrequency both can be applied in clinic.For large M-shaped rolling scars,the plasma fractional radiofrequency can be preferentially selected.
RESUMO
Objective To propose a new diagnostic criterion for rosacea based on the analysis of clinical features of rosacea in a large sample.Methods A total of 1 090 Chinese outpatients with rosacea were enrolled from Department of Dermatology of Xiangya Hospital,and their demographic data,clinical manifestations and subjective symptoms were collected.According to results of descriptive analysis,clinical features of rosacea were summarized,and a new diagnostic criterion for rosacea was set up.Then,the sensitivity and specificity of the new diagnostic criterion were verified among 1 200 outpatients clinically characterized by facial erythema.Results Of 1 090 patients with rosacea,131 (12.0％) were male and 959(88.0％) were female,and the average age was 33.5 ± 11.1 years (range,10-66).Among the 1 090 patients,715 (65.6％) had initial lesions on the cheek,of whom,712 (99.6％) had intermittent flushing as the initial symptom,and 689 (96％) had sensitive skin symptoms such as dryness,burning and itching sensations;208 (19.1％) had initial lesions on the perioral region,of whom,204 (98.1％) had persistent erythema as the initial symptom;167 (15.3％) had initial lesions on the nose,of whom,163 (97.6％) had persistent erythema as the initial symptom;in addition,311 (28.5％) had lesions on the ocular region,and only 24 (2.2％) had lesions outside the face on the neck and retroauricular region.Based on these clinical features,a new diagnostic criterion for rosacea was proposed,including 1 major condition (intermittent flushing or persistent erythema on the cheek,perioral region or nose) and 5 minor conditions (1.sensitive skin symptoms such as burning,tingling,drying or itching sensations;2.telangiectasia;3.papules or pustules;4.hypertrophy;5.ocular symptoms).If with the major condition and at least one minor condition were met,patients could be diagnosed with rosacea.After verification among 1 200 patients with facial dermatitis clinically characterized by facial erythema,the new criterion was proved to have a sensitivity of 99.3％ and a specificity of 95.8％.Conclusion A new diagnostic criterion for rosacea with high sensitivity and specificity is proposed,which is worthy of clinical application.
RESUMO
Objective:To design the small ubiquitin modification-fibroblast growth factor receptor 4 (SUMO-FGFR4) fusion gene and construct the expression vector pET22b-SUMO-FGFR4, to optimize the expression conditions. Methods:The SUMO-FGFR4 fusion gene was obtained by Overlap PCR and was connected to pET22b;the recombinant expression vector pET22b-SUMO-FGFR4 was obtained. The influence of lactose concentration, induction time,induction temperature,induction point and adding mode of lactose in the expression levels was observed,and the best induction condition was determined; then the solubility of recombinant protein was analyzed.Results:The SUMO-FGFR4 fusion protein was highly expressed,the molecular weight of the fusion protein was about 40 000 and it could bind with FGFR4 specific antibody.When the lactose concentration was 1.0 g·L-1 ,the induction time was 3 h,the induction temperature was 37℃,the value of A (600)was 0.8,the expression level was highest;but adding mode of lactose had no remarkable effect on the protein expression.The expression level of recombinant protein induced by lactose was higher than IPTG.SUMO-FGFR4 protein existed in a form of inclusion body.Conclusion:The SUMO-FGFR4 fusion protein is expressed successfully in this study while lactose is used as inducer and the best expression conditions are confirmed.