Design and Implementation of the Day Parting Appointment Registration and Examination System / 医学信息学杂志

Based on the current situation and the application situation of Intranet and Internet technologies of Gansu Provincial Maternity and Child-care Hospital,the paper takes advantage of interconnection and interworking technologies of Intranet and Internet,constructs the day parting appointment registration and examination system in a variety of ways,introduces the overall design,deployment and implementation of the system,and summarizes the application experience.This system saves greatly the time for the queuing of patients,relieves the pressure of waiting for treatment,and improves the visiting experience of patients.

Comparative study on Wnt, Notch signaling pathway molecules in primary breast cancer and matched lymph nodes metastasis stem cells / 临床与实验病理学杂志

Purpose To investigate the gene expression and significance ofβ-catenin, Cyclin D1 and Notch1 in primary breast cancer stem cells ( BCSC) and matched lymph node metastasis stem cells. Methods 30 cases of breast invasive ductal carcinoma and matched metastasis lymph nodes were made into single cell suspensions, then BCSC were separated from them by immunomagnetic sor-ting. β-catenin, Cyclin D1 and Notch1 gene expressions of Wnt, Notch signaling pathway were detected by real time PCR. ResultsThe expression of β-catenin in primary BCSC and matched lymph nodes metastasis stem cells had statistically no differences ( P >0.05), while the expression of Cyclin D1 and Notch1 in matched lymph nodes metastasis stem cells were significantly higher than the expression in primary BCSC (P<0.01, respectively). Conclusion Compared with the primary cancer stem cells, Cyclin D1 and Notch1 activation in metastasis cancer stem cells are in higher level, which leades to a higher capability of invasion and metastasis, which may be a new therapeutic target.

The human papilloma virus in endometrium cancer and its clinical significance / 中国基层医药

Objective To investigate human papilloma virus(HPV) infection in normal endometrium, atypi-cal hyperplasia of endometrium and endometrial carcinoma. Methods By the nucleic acid hybridization,we detected 28 pairs of endometrial carcinomas, 21 pairs of atypical hyperplasia of endometrium. Normal endometrium from 16 pa-tients with uterine myomas were as control. Results HPV16/18 DNA was detected in 25 endometrial carcinoma and 2 atypical hyperplasia of endometrium and 1 normal endometrium. Conclusions Endometrial carcinoma HPV16/18 DNA were significantly higher than those infected with atypical endometrial hyperplasia and normal endometrium. Note the occurrence of endometrial cancer,the development may be associated with HPV infection.