Determinant factors of lower limb explosive force among preschool children in Guangxi coastal areas in 2016 / 中国学校卫生

Objective@#To understand the main influencing factors of lower limb muscle strength development in children aged 3-6 in coastal areas of Guangxi, and to provide a reference for physical education and health management physical health work for children.@*Methods@#Using stratified cluster sampling method, a total of 15 kindergarten children perform selected from Qinzhou, Beihai and Fangcheng Gang were administered physical tests, and the parents (or guardians) received a face to face questionnaire survey, Univariate analysis and multivariate linear regression analysis were used to analyze determinant factors of lower limbs explosive force.@*Results@#The correlation analysis of the factors affecting lower limb explosive force of children aged 3-6 years showed that there were significant differences in the correlation of 13 indicators including gender, age, height, weight, birth weight and body length, gestational age, parents’ education level, parents’ age, and mother’s height and weight(P<0.05). Furthermore, multiple linear regression analysis showed that age, gender, weight, height, father’s education level and mother’s age had significant effects on lower limb explosive force of 3-6 years old infants in Guangxi coastal areas(P<0.05).@*Conclusion@#Growth and development, as well as paternal educational level plays an important role in the development of lower limb explosive force of children aged 3-6 in coastal areas of Guangxi, while weight and length at birth, parental height and weight shows no association with it.

The Preventive Effects of Combined Nicorandil With Trimetazidine on Contrast-induced Nephrophathy in Patients Undergoing Elective Percutaneous Coronary Intervention / 中国循环杂志

Objectives: To observe the preventive effects of combined nicorandil with trimetazidine strategy on contrast-induced nephrophathy in patients undergoing elective percutaneous coronary intervention (PCI). Methods: 521 patients with acute coronary syndrome undergoing elective PCI were randomly divided into 4 groups:control group (n=208), nicorandil group (n=105), trimetazidine group (n=104), nicorandil plus trimetazidine group (n=104). The control group received hydration and aspirin, β-blockers, statins and other conventional drugs for coronary heart disease. The remaining three groups were given respective medication on top of the medications used in control group at 48 hours prior to PCI. The levels of respective blood index were measured and compared among four groups at preoperative and at 72 hours post operation. The primary endpoint was the incidence of CIN. Results: The incidence of contrast-induced nephropathy (CIN) in the control group, nicorandil group, trimetazidine group, nicorandil plus trimetazidine group were 8.2%, 2.8%, 3.8%, 1.0% respectively, which was significantly lower in nicorandil plus trimetazidine group than in the control group (P=0.009). Multivariate logistic regression analysis showed that, compared with control group, the use of nicorandil plus trimetazidine strategy was related to decreased CIN incidence (OR=0.114, 95%CI: 0.015-0.880, P=0.037). Conclusions: In patients with acute coronary syndrome undergoing elective PCI, the use of nicorandil plus trimetazidine strategy is related to decreased CIN incidence.

The Role of NM23 in Patients with Colorectal Cancer: A Systematic Review and Meta-Analysis / 华中科技大学学报（医学）（英德文版）

This meta-analysis was carried out to evaluate the relationship between NM23 expression and the prognosis of patients with colorectal cancer.We searched PubMed,EMBASE and Web of Science for relevant articles.The pooled odd ratios (ORs) and corresponding 95％CI were calculated to evaluate the prognostic value of NM23 expression in patients with colorectal cancer,and the association between NM23 expression and clinicopathological factors.In total,2289 patients were pooled from 24 available studies.The incorporative OR combined by 16 studies with overall survival showed that high NM23 expression was associated with better overall survival (OR=0.67,95％CI:0.49-0.93,P=0.02,I2=56％,Ph=0.004).And a new estimate without heterogeneity was produced when only combining high-quality studies (OR=0.70,95％CI:0.56-0.86,P=0.0007,I2=46％).In disease free survival (DFS),we also obtained a good prognosis (OR=0.30,95％CI:0.14-0.68,P=0.004).Although we failed to find any significance in N status (P=0.10),elevated NM23 expression was related to well tumor differentiation (OR=0.60,95％CI:0.44-0.820,P=0.001) and Dukes' A&B (OR=0.55,95％CI:0.32-0.95,P=0.03).These results indicated that over-expressed NM23 might be an indicator of good prognosis,well tumor differentiation and Dukes' A&B of patients with colorectal cancer,but no significance was found in N status.

Correlation between osteoprotegerin gene rs2073617T/C, rs2073618G/C polymorphism and acute coronary syndromes in Fujian Han population / 解放军医学杂志

Objective To study the genotype distribution and its association with acute coronary syndrome (ACS) of polymorphism of osteoprotegerin (OPG) gene promoter rs2073617T/C(950T/C) and the first exon rs2073618G/C(1181G/C) in Fujian Han population. Methods A total of seven hundred and twenty consecutive patients of unrelated Fujian Han people with ACS were enrolled in the present study. They were divided into ACS group and control group, 360 each. OPG gene 950T/C and 1181G/C were genotyped in all the patients using polymerase chain reaction restriction fragment length polymorphism (PCRRFLP). DNA sequences of enzyme digestion products were also analyzed. Results In Fujian Han population, the frequency of TC, TT, CC types of OPG 950T/C polymorphism in ACS patients and controls were 47.8%, 26.7% and 25.5% vs 43.3%, 33.3% and 23.3%; The frequency of GG, GC, CC types of OPG 1181G/C polymorphism in ACS patients and controls were 51.1%, 40.0% and 8.9% vs 60.0%, 35.0% and 5.0%. No significant differences were observed in the genotype and allelic frequency of OPG gene 950T/C and 1181G/C between the ACS and control groups. OPG gene 950T/C and 1181G/C showed no statistically significant difference between ACS patients with single-vessel, double-vessel and three or more-vessel disease. Conclusion OPG gene polymorphism of 950T/C and 1181G/C are not associated with ACS in Han population of Fujian.

Correlation between osteoprotegerin gene rs2073617T/C, rs2073618G/C polymorphism and acute coronary syndromes in Fujian Han population / 解放军医学杂志

Objective To study the genotype distribution and its association with acute coronary syndrome (ACS) of polymorphism of osteoprotegerin (OPG) gene promoter rs2073617T/C(950T/C) and the first exon rs2073618G/C(1181G/C) in Fujian Han population. Methods A total of seven hundred and twenty consecutive patients of unrelated Fujian Han people with ACS were enrolled in the present study. They were divided into ACS group and control group, 360 each. OPG gene 950T/C and 1181G/C were genotyped in all the patients using polymerase chain reaction restriction fragment length polymorphism (PCRRFLP). DNA sequences of enzyme digestion products were also analyzed. Results In Fujian Han population, the frequency of TC, TT, CC types of OPG 950T/C polymorphism in ACS patients and controls were 47.8%, 26.7% and 25.5% vs 43.3%, 33.3% and 23.3%; The frequency of GG, GC, CC types of OPG 1181G/C polymorphism in ACS patients and controls were 51.1%, 40.0% and 8.9% vs 60.0%, 35.0% and 5.0%. No significant differences were observed in the genotype and allelic frequency of OPG gene 950T/C and 1181G/C between the ACS and control groups. OPG gene 950T/C and 1181G/C showed no statistically significant difference between ACS patients with single-vessel, double-vessel and three or more-vessel disease. Conclusion OPG gene polymorphism of 950T/C and 1181G/C are not associated with ACS in Han population of Fujian.

Chromosome 1p/19q status combined with expression of p53 protein improves the diagnostic and prognostic evaluation of oligodendrogliomas / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>Our previous study confirmed that oligodendrogliomas had higher frequency of chromosome 1p/19q deletion. In order to improve the diagnostic criteria and to predict the prognosis of oligodendroglioma patients, the status of chromosome 1p/19q deletion, the methylation of O(6)-methylguanine-DNA methyltransferase (MGMT), and the expression of p53 protein were evaluated and investigated in relation to patients' outcomes.</p><p><b>METHODS</b>Methylation of MGMT in 73 cases was analyzed by nested methylation-specific PCR (MSP). The levels of MGMT and p53 protein were tested with immunohistochemistry. Pearson's chi-square test and Fisher's exact test were used. Multivariate and Kaplan-Meier analysis were performed to determine patients' outcomes.</p><p><b>RESULTS</b>Both oligodendrogliomas and astrocytic gliomas exhibited frequent methylation of MGMT. However, the results of MSP did not completely correspond to that of the immunohistochemical staining for MGMT. The expression of p53 protein was more frequently observed in patients without a 1p or 19q deletion in anaplastic oligodendrogliomas (P = 0.032, 0.025). In low-grade oligodendrogliomas, methylation of MGMT was more frequent in patients with 1p/19q deletion than in patients with 1p/19q intact (P = 0.038). Patients with oligodendrogliomas with 1p/19q loss of heterozygosity and p53-negative showed a longer progression-free survival.</p><p><b>CONCLUSION</b>Detection of chromosome 1p/19q status combined with p53 protein immunohistochemistry might be beneficial to improve the pathological diagnosis and to determine the prognosis of patients with oligodendrogliomas.</p>

Loss of heterozygosity of chromosome 1p/19q and p53 protein expression in oligodendroglioma / 中华病理学杂志

<p><b>OBJECTIVE</b>To study the status of loss of heterozygosity (LOH) of chromosome 1p/19q and p53 protein expression in oligodendroglioma, as compared to astrocytoma.</p><p><b>METHODS</b>One hundred and ninety-one cases of glioma of different histologic types and grades, including 116 cases of low-grade of oligodendroglioma (86 paraffin-embedded and 30 fresh tissues), 45 cases of anaplastic oligodendroglioma (all paraffin-embedded tissues) and 30 cases of astrocytoma of various grades (all paraffin-embedded tissues), were enrolled into the study. The LOH of chromosome 1p/19q was investigated by polymerase chain reaction (PCR)-based microsatellite analysis. The p53 protein expression was demonstrated by immunohistochemical staining.</p><p><b>RESULTS</b>The rates of 1p loss, 19q loss and 1p/19q loss were 69.8%, 64%, and 57.0% respectively in the 86 paraffin-embedded low-grade oligodendroglioma samples, as compared to 71.1%, 60.0% and 55.6% respectively in the 45 paraffin-embedded anaplastic oligodendroglioma samples. There was no difference of LOH of 1p/19q between low-grade oligodendroglioma and anaplastic oligodendroglioma (P>0.05). In the 30 cases of low-grade oligodendroglioma with fresh tissues available, the rates of 1p loss, 19q loss and 1p/19q loss were 70.0%, 63.3% and 60.0% respectively. The LOH of 1p/19q between paraffin-embedded and fresh samples was not statistically significant (P>0.05). In the 30 cases of astrocytoma, the rates of 1p loss, 19q loss and 1p/19q loss were 23.3%, 33.3% and 20.0% respectively, which were significantly less than those in oligodendroglioma (P<0.05). The expression of p53 protein was significantly lower in low-grade oligodendroglioma (8.1%) than in anaplastic oligodendroglioma (31.1%, P=0.007). The expression of p53 protein in oligodendroglioma was also lower than in astrocytoma (P=0.001). Furthermore, p53 protein expression negatively correlated with 1p/19q loss in anaplastic oligodendroglioma (P<0.05).</p><p><b>CONCLUSIONS</b>Both paraffin-embedded and fresh tissues are suitable for analysis of LOH of chromosome 1p/19q. Oligodendroglioma demonstrates a higher frequency of LOH of chromosome 1p/19q and lower expression of p53 protein than astrocytoma. The LOH of chromosome 1p/19q negatively correlates with the expression of p53 protein. These parameters have both diagnostic and prognostic values.</p>

Effects of P2Y1 receptor on glial fibrillary acidic protein and glial cell line-derived neurotrophic factor production of astrocytes under ischemic condition and the related signaling pathways / 神经科学通报·英文版

<p><b>OBJECTIVE</b>The present study aimed to explore the role of P2Y(1) receptor in glial fibrillary acidic protein (GFAP) production and glial cell line-derived neurotrophic factor (GDNF) secretion of astrocytes under ischemic insult and the related signaling pathways.</p><p><b>METHODS</b>Using transient right middle cerebral artery occlusion (tMCAO) and oxygen-glucose-serum deprivation for 2 h as the model of ischemic injury in vivo and in vitro, immunofluorescence, quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR), Western blotting, enzyme linked immunosorbent assay (ELISA) were used to investigate location of P2Y(1) receptor and GDNF, the expression of GFAP and GDNF, and the changes of signaling molecules.</p><p><b>RESULTS</b>Blockage of P2Y(1) receptor with the selective antagonist N(6)-methyl-2'-deoxyadenosine 3',5'-bisphosphate diammonium (MRS2179) reduced GFAP production and increased GDNF production in the antagonist group as compared with simple ischemic group both in vivo and in vitro. Oxygen-glucose-serum deprivation and blockage of P2Y(1) receptor caused elevation of phosphorylated Akt and cAMP response element binding protein (CREB), and reduction of phosphorylated Janus kinase2 (JAK2) and signal transducer and activator of transcription3 (STAT3, Ser727). After blockage of P2Y(1) receptor and deprivation of oxygen-glucose-serum, AG490 (inhibitor of JAK2) reduced phosphorylation of STAT3 (Ser727) as well as expression of GFAP; LY294002, an inhibitor of phosphatidylinositol 3-kinase (PI3-K), decreased phosphorylation of Akt and CREB; the inhibitor of mitogen-activated protein kinase kinase1/2 (MEK1/2) U0126, an important molecule of Ras/extracellular signal-regulated kinase (ERK) signaling pathway, decreased the phosphorylation of JAK2, STAT3 (Ser727), Akt and CREB.</p><p><b>CONCLUSION</b>These results suggest that P2Y(1) receptor plays a role in the production of GFAP and GDNF in astrocytes under transient ischemic condition and the related signaling pathways may be JAK2/STAT3 and PI3-K/Akt/CREB, respectively, and that crosstalk probably exists between them.</p>

The level of transgelin related to the function of dendritic cells pulsed with liver cancer / 中华肝脏病杂志

<p><b>OBJECTIVE</b>To explore the relationships between the expression of transgelin in dendritic cells (DCs) pulsed with hepatocellular carcinoma lysates and the functions of the DCs.</p><p><b>METHODS</b>DCs derived from healthy human white blood cells were divided into 3 groups: one was pulsed with high metastatic potential hepatocellular carcinoma cell line (MHCC97H) lysates, one with lysates of a low metastatic potential cell line (MHCC97L), and one un-pulsed DCs served as the control. The morphology of the DCs was observed by confocal microscopy and scanning electron microscopy. The phenotypes of the DCs were detected by flowcytometric analysis. The mixed leucocyte reaction (MLR) test and IL-12 secretion of DCs in the supernatants of MLR were employed to determine the functions of the DCs; the expression of transgelin was detected by Western blot.</p><p><b>RESULTS</b>There were no morphological changes in the different DCs, but the levels of HLA-DR, CD80, CD83, CD86, MLR and IL-12 and transgelin were significantly higher in the two pulsed groups than those in the control group (P less than 0.01). In MHCC97H pulsed DCs, their CD80, CD83, CD86, and the expression of transgelin were also higher than those in the control group (P less than 0.05). The expression of transgelin was significantly higher in the MHCC97H pulsed group than in the MHCC97L loaded group, but CD80, CD83, CD86 and the level of IL-12 were all lower in the MHCC97H loaded DC group in comparison with those in the MHCC97 pulsed group (P less than 0.05).</p><p><b>CONCLUSION</b>The expression of transgelin in DCs pulsed with HCC lysates is related to the functions of the DCs.</p>

Involvement of MAPK/ERK kinase-ERK pathway in exogenous bFGF-induced Egr-1 binding activity enhancement in anoxia-reoxygenation injured astrocytes / 神经科学通报·英文版

<p><b>OBJECTIVE</b>Intravenous administration of basic fibroblast growth factor (bFGF) is effective to reduce the volume of cerebral infract due to ischemia. This study was designed to investigate the molecular mechanism, especially the signal transduction pathways, involved in this protective role of bFGF.</p><p><b>METHODS</b>Anoxia-reoxygenation treated astrocytes were used to study the role of mitogen-activated protein kinase/extracellular signal-regulated kinase kinase (MAPK/ERK kinase, MEK)-ERK signaling pathway after exogenous bFGF administration by Western blot. Electrophoretic mobile shift assay was used to detect the binding activity of early growth response factor-1 (Egr-1), an important transcription factor for endogenous bFGF.</p><p><b>RESULTS</b>bFGF could protect some signal transduction proteins from the oxygen-derived free radicals induced degradation. ERK1/2 was activated and involved in Egr-1 binding activity enhancement induced by exogenous bFGF.</p><p><b>CONCLUSION</b>MEK-ERK MAPK cascade may be an important signal transduction pathway contributed to bFGF induced enhancement of Egr-1 binding activity in anoxia-reoxygenation injured astrocytes.</p>

Growth enhancement effect of BzATP on primary cultured astrocytes from rat brain / 神经科学通报·英文版

<p><b>OBJECTIVE</b>To explore whether BzATP could promote the growth of primary cultured astrocytes (AS) of rat and its possible mechanism, and whether TGF-β1 was involved in the event.</p><p><b>METHODS</b>The primary cultured ASs were derived from new born Sprague-Dawley rats. Glial fibrillary acidic protein (GFAP) immunofluorescent staining was used to check the purity of cultured AS. Morphometry was used to detect the changes of AS. The proliferation index of AS was detected by BrdU incorporation assay. Western blot was used to detect the changes of GFAP under different conditions. Changes of TGF-β1 gene transcription were detected by RT-PCR. ELISA was utilized to detect the variation of TGF-β1 protein in the supernate.</p><p><b>RESULTS</b>The purity of primary cultured AS reached 99%. BzATP promoted the hypertrophy of AS including the elongation of AS processes and the enlargement of cell bodies, BzATP also promoted the expression of GFAP in existence of Ca²⁺, but had no effect on cell proliferation. BzATP increased the transcription of TGF-β1 mRNA and the release of TGF-β1 protein in existence of Ca²⁺. TGF-β1 neutralizing antibody partially inhibited the expression of GFAP induced by BzATP, but had no effect on AS proliferation and cell morphology.</p><p><b>CONCLUSION</b>BzATP enhanced the hypertrophy of primary cultured AS, increased the expression of GFAP partially through TGF-β1. Mechanisms of the enhancement of AS growth induced by BzATP other than TGF-β1 pathway remain to be elucidated.</p>