Reparo de lesão penetrante em parede torácica de cão, ocasionada por javali (Sus scrofa scrofa), com flape unipediculado de músculo reto abdominal / Unipediculated flap of muscle rectos abdominales to repair a penetrating injury caused by javali (Sus scrofa scrofa) on the thoracic wall of a dog

Lesões penetrantes no tórax causadas por interação animal são frequentes em cães e caracterizam-se por dano extenso e desvitalização dos tecidos moles adjacentes. Quando a musculatura local é insuficiente para a reconstrução, retalhos musculares podem ser mobilizados para reparar o defeito torácico. O presente relato tem como objetivo demonstrar uma alternativa para o reparo de defeito da parede torácica em um cão, ocasionada por interação com javali (Sus scrofa scrofa), utilizando flape unipediculado de músculo reto abdominal. Com base na literatura consultada, essa técnica reconstrutiva ainda não foi descrita. O flape de músculo reto abdominal mostrou-se uma alternativa viável no reparo de lesão extensa e infectada na parede torácica em cães.(AU)

Penetrating thoracic wounds caused by animal bites are common in dogs and are characterized by extensive trauma and adjacent soft tissue devitalization. When the local musculature is insufficient for the reconstruction, muscle flaps can be taken to repair the thoracic defect. The aim of the present report is to demonstrate an alternative to the thoracic wall defect repair in a dog which was attacked by javali (Sus scrofa scrofa), using unipediculated flap of the rectus abdominis muscle. Based on the literature, this reconstructive technique has not yet been described. The rectus abdominis muscle flap proves to be a useful alternative for the repair of extensive and infected thoracic wall lesion in dogs.(AU)

Células-tronco mononucleares associadas ao plasma rico em plaquetas na consolidação de falha óssea no cão. Estudo piloto / Mononuclear stem cells associated with platelet-rich plasma in the consolidation of bone defects in dogs. A pilot study

A padronização de técnicas e o teste da associação do Plasma Rico em Plaquetas (PRP) e das Células-Tronco Mononucleares (CTMs) na consolidação de falhas ósseas corticais, por meio de avaliação clínica, biomecânica, radiológica e histológica, é avaliada em um estudo piloto. Foram utilizados seis cães adultos, fêmeas, sem raça definida, pesando entre 5 e 10kg, separados por sorteio aleatório em seis tratamentos. Foi confeccionada uma falha elíptica de 1,0x0,4cm na cortical medial diafisária da tíbia direita de cada animal, sendo preenchida de acordo com o tratamento proposto. No cão I, a falha foi preenchida com solução fisiológica (SF); no II, com o PRP; no III, com a fração total das células mononucleares (FTCM); no IV, com a fração vascular estromal (FVE); no V, com o PRP associado à FTCM; no VI, com a associação PRP e FVE. Foram realizadas avaliações: clínicas, diariamente; dos graus de claudicação, semanalmente; radiológica e perimetria da coxa, antes, no pós-operatório imediato, aos 7, 14, 21 e 30 dias; biomecânica, antes do procedimento, aos 10, 20 e 30 dias; e biópsias, aos 15 e 30 dias. A FTCM obteve uma contagem e viabilidade média de 2,0x108cél. e 90%, respectivamente, enquanto a FVE obteve 3x106cél. e 50%. O PRP concentrou, em média, sete vezes o número inicial de plaquetas do sangue total, de 250.000 µl-1 plaquetas no sangue total para 1.750.000 µl-1 plaquetas no PRP. Obteve-se padronização adequada de técnicas, possibilitando o teste da associação entre as células-tronco mononucleares (CTMs) e o plasma rico em plaquetas (PRP), assim como seu uso isolado, no reparo de falhas ósseas corticais, indicando a possibilidade de a associação FTCM e PRP ser o melhor tratamento...

The standardization of techniques and tests of the association of Platelet Rich Plasma (PRP) and Mononuclear Stem Cells (MSCs) in the consolidation of cortical bone defects by clinical, biomechanical, radiological, and histological analysis is evaluated in a pilot study. Six adult female dogs of mixed breed, weighing between 5 and 10kg, separated by random draw in six treatments were used. An elliptical failure of 1.0 x0.4cm was done in the medial diaphyseal cortical of the right tibia of each animal, that was filled according with the proposed treatment. In dog I, the failure was filled with saline (S), in dog II with PRP, in dog III with total mononuclear cell fraction (TMCF), in dog IV with stromal vascular fraction (SVF), in dog V with association of PRP and TMCF, and in dog VI with an association of PRP and SVF. Daily clinical evaluation, weekly degrees of lameness, radiological and girth before, immediate postoperative, 7, 14, 21 and 30 days, biomechanics before the procedure, at 10, 20 and 30 days, and biopsies at 15 and 30 days were performed. The TMCF got a count and viability of 2,0x108cells and 90% respectively, while for SVF it was 3x106cells and 50%, respectively. The PRP concentrated on average seven times the original number of platelets from whole blood, platelets from whole blood 250.000 μl-1 to 1.750.000 μl-1 platelets in PRP. This afforded adequate standardization of techniques, enabling the test of association between mononuclear stem cells (MSCs) and platelet-rich plasma(PRP), as well as their separate use to repair cortical bone defects, indicating the possibility of the association between FTCM and PRP to be the best treatment...

Nível de dano econômico para formigas-cortadeiras em função do índice de produtividade florestal de eucaliptais em uma região de Mata Atlântica / Economic damage level for leaf-cutting ants in function of the productivity index of eucalyptus plantations in an Atlantic Forest region

The production and quality of eucalyptus plantations have been studied in areas with different densities of ant nests, being important to estimate losses caused by leaf-cutting ants. The effects of leaf-cutting ant on wood production in differents productivity sites were studied in eucalyptus plantations in the region of Atlantic Forest, Minas Gerais State, Brazil from 2003 to 2006. Data of plots of the continuous forest inventory and data of leaf-cutting ant monitoring in eucalyptus plantations were obtained. Each unitary increment in the area of Atta spp. nests per hectare reduced the wood production of the eucalyptus forest between 0.04 and 0.13 m³.ha-1, resulting in a level of economic damage for leaf-cutting ants between 13.4 and 39.2 m².ha-1, in this region. Moreover, this study innovated when using indices of forest productivity (site index) that promote better adjustment of the models and produce estimate more accurate of the level of economic damage for leaf-cutting ants in cultivated forests, allowing to conclude that the increase of the total area of ant nests reduces the wooden volume of eucalyptus, proportionally to the productive potential of the forest.

Sobrevivência de operários do cupim-de-montículo Cornitermes cumulans (Kollar, 1832) (Isoptera: Termitidae) alimentados com diferentes dietas artificiais / Survival of workers of the termite Cornitermes cumulans (Kollar, 1832) (Isoptera: Termitidae) fed with different diets

Avaliou-se a sobrevivência de operários do cupim Cornitermes cumulans (Kollar, 1832), isolados da colônia e mantidos em diferentes dietas artificiais em laboratório (21 ± 1° C; 90±10% UR e 24h de escotofase), em Lavras, Minas Gerais, Brasil. Nos bioensaios ofereceram-se ou não as dietas a 50 indivíduos por placa de Petri plástica com tampas perfuradas. Foram utilizadas seis repetições por tratamento, avaliando-se diariamente a sobrevivência dos cupins até a morte de todos os indivíduos. Os resultados obtidos foram submetidos à análise de sobrevivência de Weibull e as equações submetidas ao teste de identidade de modelos não-lineares. Houve diferença na sobrevivência estimada dos cupins tratados com as diferentes dietas testadas. Concluiu-se que é possível manter operários de Cornitermes cumulans vivos e isolados da colônia com o emprego de dieta contendo papel filtro, bagaço de cana-de-açúcar e ágar como fonte de alimento para esses cupins.

We evaluated the survival of the termite Cornitermes cumulans (Kollar, 1832), isolated from the colony and kept on different artificial diets in the laboratory (21 ± 1° C, 90 ± 10% UR and 24h of scotophase), in Lavras, Minas Gerais, Brazil. Fifty termite workers were isolated in each plastic Petri dish, where they were offered diets or not. We used 6 replicates per treatment, evaluating the daily survival of termites until the death of all the individuals. The results were submitted to Weibull survival analysis and the equations were subjected to the test of identity of non-linear models. There was a difference in estimated survival of termites treated with different diets. It was concluded that it is possible to keep Cornitermes cumulans workers alive and isolated from the colony with the use of a diet containing filter paper, bagasse cane sugar and agar as a food source.

Hernia de disco toracica operada por toracoscopia videoassistida / Herniated thoracic disc operated through video-assisted thoracoscopy

A detailed description of the surgical steps od the first five patients with herniated thoracic disc operated on through videoendoscopic technique at our institution are described below: This is an optimal approach to the spine through a ventral route with minimally ivasive technique. This surgical modality allows for superb illumination, visualization and magnification and permits relatively easy manipulation and dissection of delicate amatomic structures through small openings and with the use of high resolution endoscopes. Fast recovery is usual and the patients were discharged on the 3rd ou 4th postoperative day, some of them claiming of intercostal pain for more 24 to 48 hours. No cases of infection were observed.