Assuntos
Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Meningites Bacterianas/diagnóstico , Meningites Bacterianas/microbiologia , Infecções Estreptocócicas/diagnóstico , Infecções Estreptocócicas/microbiologia , Streptococcus suis/isolamento & purificação , Chile/epidemiologia , Meningites Bacterianas/epidemiologia , Infecções Estreptocócicas/epidemiologia , Streptococcus suis/genéticaRESUMO
The cassette chromosome mec (SCCmec) present in methicillin-resistant Staphylococcus aureus (MRSA) has two essential components, the ccr gene complex and the mec gene complex. Additionally, SCCmec has non-essential components called J regions which are used for MRSA subtyping. This study was performed to determine subtypes MRSA strains carrying SCCmec type I based on polymorphism of regions located downstream of the mecA gene. A total of 98 MRSA strains carrying SCCmec type I isolated from patients hospitalized at the County Hospital of Valdivia (Chile) between May 2007 and May 2008, were analyzed by multiplex PCR designed to amplify the mecA gene and 7 DNA hypervariable regions located around the mecA gene. MRSA strains were classified into seventeen genotypes accordingly to amplification patterns of DNA hypervariable regions. Five genotypes showed amplification patterns previously described. The remaining twelve genotypes showed new amplification patterns. Genotypes 18 and Genotype 19 were the most frequently detected. Regions HVR, Ins117 and pI258 stand out as being present in more than 60% of tested isolates. The acquisition of hypervariable regions by MRSA is a continuous horizontal transfer process through which the SCCmec have been preserved intact, or even may give rise to new types and subtypes of SCCmec. Therefore it is possible to infer that most MRSA strains isolated at the County Hospital of Valdivia (Chile) were originated from two local clones which correspond to Genotype 18 and Genotype 19.
Assuntos
Humanos , Antibacterianos/análise , Sequência de Bases , Resistência Microbiana a Medicamentos , Técnicas In Vitro , Polimorfismo Genético , Reação em Cadeia da Polimerase/métodos , Resistência a Meticilina/genética , Infecções Estafilocócicas , Staphylococcus aureus/genética , Staphylococcus aureus/isolamento & purificação , Genótipo , Métodos , PacientesRESUMO
Actualmente , se reconocen 32 especies de Staphylococcus y 8 variedades. La mitad se aisla del hombre, siendo su hábitat el ecosistema cutáneo. Muchas de estas especies son reportadas, produciendo diferentes cuadros, sea por su capacidad de adhesión, como también por los factores predisponentes del huésped y multirresistencia. Poca es la información, al respecto, en Chile. Se identificaron 106 de Staphylococcus coagulasa negativa (SNC), correspondio el 57,5 por ciento a S. epidermidis, el 11,3 por ciento a S. lugdunesis, 6,6 por ciento a S. saprophyticus. Se aisló también S. auricularis, S. cohnii, S. capitis ssp. ureolyticus, S. schleiferi y S. simulans. Se insiste en la correcta identificación de las especies. Los esquemas en uso conducen a error