Influence of acacia nilotica on Arsenic induced genotoxicity in male and female mice

For centuries, plants have been used in traditional medicine and there has been recent interest in the chemopreventive properties of compounds derived from plants. In the present study, we investigated the effects of extracts of Acacia nilotica leaves on the genotoxicity of arsinic. Arsenic contamination in groundwater a global human health hazard .There is no effective remedial action of chronic arsenicsis. However, a well-nourished diet can modulate the onset of adverse health effects and delayed the effect of arsenic in drinking water. In the present work, genotoxic effects were induced by sodium arsenate through oral administration,and the protective effect of Acacia nilotica was studied. Chromosomal aberrations were more pronounced in sodium arsenate treated mice, while supplementation of Acacia nilotica with sodium arsenate reduced the incidence of the aberrations. The mean of DNA fragmentation induced by sodium arsenate was highly significant increase. However, the administration of Acacia nilotica significantly decreased DNA fragmentation induced by sodium arsenate. The mean number of sperms, were decreased significantly after treatment with sodium arsenate, while administration of Acacia nilotica increased the number of sperm in mice treated with sodium arsenate, and also decreased the percentage of sperm abnormalities induced by sodium arsenate. The outcome of study showed that Acacia nilotica has the efficiency to encounter the genotoxic effects induced by arsenic

role of melatonin in aluminium induced genotoxicity in female albino rats

In spite of the wide range of use of aluminium. With the increasing awareness of possible health hazards associated with their use, more attention have been focused on their biological and biochemical activity. Aluminium salts are used as the active antiperspirant in under cosmetics, but the effects of widespread, long term and increasing use remain unknown. Aluminium is known to have genotoxic profile, capable of causing both DNA alterations and epigenetic effects. Melatonin reduces the free radical generation and metal detoxification. This study was planned to determine the effects of melatonin on aluminium induced toxicity in the chromosomes of bone marrow cell. Forty female albino rats were used in the present work for the chromosomal analysis. Four groups of adult female albino rats were used in the following way: Group -I, control group; Group-Il, 50 mg /kg b .wt aluminium orally; Group-Ill, 50 micro g /kg b .wt melatonin orally and Group- IV, 50 mg /kg b .wt aluminium orally [am] +50 micro g I kg b .wt melatonin orally [pm]. Doses were given 30 days. All animals were sacrificed and the bone marrow of femur bones was taken for subsequent cytogenetic studies. Aluminium treatment induced a significant increase in the structural as well as numerical chromosomal aberrations. The frequency of chromosomal aberrations showed significant decrease when rats treated with melatonin and aluminium. The results indicated that melatonin showed a significant improvement of genetic damages induced by aluminium