The FOXO1 Gene-Obesity Interaction Increases the Risk of Type 2 Diabetes Mellitus in a Chinese Han Population

Here, we aimed to study the effect of the forkhead box O1-insulin receptor substrate 2 (FOXO1-IRS2) gene interaction and the FOXO1 and IRS2 genes-environment interaction for the risk of type 2 diabetes mellitus (T2DM) in a Chinese Han population. We genotyped 7 polymorphism sites of FOXO1 gene and IRS2 gene in 780 unrelated Chinese Han people (474 cases of T2DM, 306 cases of healthy control). The risk of T2DM in individuals with AA genotype for rs7986407 and CC genotype for rs4581585 in FOXO1 gene was 2.092 and 2.57 times higher than that with GG genotype (odds ratio [OR] = 2.092; 95% confidence interval [CI] = 1.178–3.731; P = 0.011) and TT genotype (OR = 2.571; 95% CI = 1.404–4.695; P = 0.002), respectively. The risk of T2DM in individuals with GG genotype for Gly1057Asp in IRS2 gene was 1.42 times higher than that with AA genotype (OR = 1.422; 95% CI = 1.037–1.949; P = 0.029). The other 4 single nucleotide polymorphisms (SNPs) had no significant association with T2DM (P > 0.05). Multifactor dimensionality reduction (MDR) analysis showed that the interaction between SNPs rs7986407 and rs4325426 in FOXO1 gene and waist was the best model confirmed by interaction analysis, closely associating with T2DM. There was an increased risk for T2DM in the case of non-obesity with genotype combined AA/CC, AA/AC or AG/AA for rs7986407 and rs4325426, and obesity with genotype AA for rs7986407 or AA for rs4325426 (OR = 3.976; 95% CI = 1.156–13.675; P value from sign test [P(sign)] = 0.025; P value from permutation test [P(perm)] = 0.000–0.001). Together, this study indicates an association of FOXO1 and IRS2 gene polymorphisms with T2DM in Chinese Han population, supporting FOXO1-obesity interaction as a key factor for the risk of T2DM.

Association of polymorphisms in transcription factor 7-like 2(TCF7L2) gene with type 2 diabetes in Chinese Han population / 中华内分泌代谢杂志

Objective To study the association of transcription factor 7-like 2(TCF7L2)polymorphisms with tvpe 2 diabetes mellitus in Chinese Han population. Methods Two polymorphisms (rs7903146 and rs12255372)of TCF7L2 gene were genotyped in 446 patients with type 2 diabetes mellitus(T2DM group)and 303 normal subiects (NC group) by PCR-restriction fragment length polymorphism(PCR-RFLP).Waist circumference.body mass index,plasma glucose,serum insulin,lipid profiles,high-sensitivity C-reactive protein and non-esterified fatty acid were measured.Homeostasis model assessment of insulin resistance(HOMA-IR)and β-cell function(HOMA-β)were calculated.Results (1) In T2DM group,T allele frequency and CT,TY geno tvpe frequeneies of rs7903146 were significantly higher than those in NC group(0.093,0.150,0.018 vs 0.043, 0.079,0.003,respectively,a11 P<O.O 1).Logistic regression analysis showed that the CT/TT genotype was a risk factor of tvpe 2 diabetes(OR=2.25,95%CI 1.39-3.62,P=0.001)and was associated with the decrease of insulin secretion. (2) No significant association was observed in vs12255372 alleles and genotypes with type 2 diabetes mellitus.Conclusion These results indicate that TCF7L2 might be one of the candidate genes for confe ring susceptibi lity to type 2 diabetes mellitus in the Chinese Han population.

Association of FcRL3 gene polymorphism with autoimmune thyroid diseases / 中华内分泌代谢杂志

The polymorphism in the exon 4 of FcRL3 gene was evaluated by PCR-FPLR in 506 patients with Graves' disease (GD), 80 with Hashimoto thyroiditis (HT) and 261 normal subjects in Chongqing. The data suggest that 82G allele in exon 4 of FcRL3 gene may be susceptible to GD in male patients of Chongqing Hart nationality.

Evaluation of bladder function after gastrocystoplasty / 重庆医科大学学报

Objective:To evaluate the function of bladder after gastrocystoplasty.Methods:38 patients after gastrocystoplasty were analyzed by observing the micturition and examination of urodynamics and to investigate the function of bladder during the filling and the micturition.Results:In the 38 patients,voiding interval time was 3.2?1.7hours;unirary output was 320?110ml;maximual flow rate was 19?7.2 ml per second;intravcsical pressure during filling was 11.2?8.2 cmH 2O;maximual intravesical pressure during voiding was 58?21 cmH 2O;and maximual pressure of detrusor was 28?12 cmH 2O.In total 38 cases,residual urine was observed in 11 cases,and residual volume was 55?34ml.12 cases developed uracratia or enuresis.Conclusion:Filling function of bladder is satisfactory after gastrocystoplasty,the micturition is carried out mainly by the action of intra-abdominal pressure.The disturbance of function of the sensation of bladder and the urethral sphincter is possibly the common cause for uracratia or enuresis.

Analysis of the expression of ornithine decarboxylase antizyme inhibitor in mouse tissues / 重庆医科大学学报

Objective:Analysis of the expression of Ornithine decarboxylase antizyme inhibitor in mouse tissues.Methods:RT-PCR,Northern blot,immunostaining.Results:RT-PCR,Northern blot reveal that the expression of Ornithine decarboxylase antizyme inhibitor gene(AZI) is ubiquitous,especially high in brain,heart,kidney,muscle,thymus and testis.Immunostaining reveals that AZI expresses on valve cells in heart,mesenchyme cells in kidney and liver.Conclusion:The expression of AZI in mouse tissues was identified by using RT-PCR,Northern blot and immunostaining.