Expression of p53 in breast cancer and regulatory effects of 5-aza-2′-deoxycytidine on p53 / 医学研究生学报

Objective Tumor suppressor gene p53 can inhibit tumor cell growth, arrest cell cycle, and promote apoptosis.Howev-er, the effects of p53 on the pathogenesis of breast cancer have not been fully elucidated.The aim of this study was to explore the expression of p53 protein and the correlation with clinical pathologic features in breast cancer.Furthermore, the regulatory effects of 5-aza-2′-deoxycyti-dine on p53 in breast cancer cell line were also studied. Methods The expression of p53 protein in 80 cases of breast cancer and normal and adjacent tissue were determined by the immunohistochemical staining .The expressions of p53 mRNA and p53 protein in breast cancer cell line were determined by RT-PCR and Western blotting. Results The positive rate of p53 in breast cancer (41.25%) was higher than that in the normal and adjacent tissue (22.5%) (P0.05).The low expression of p53 both in mRNA and in protein levels were found in breast cancer cell line of MCF-7.The expres-sion of p53 increased after 5-aza-2′-deoxycytidine administration . Conclusion p53 is highly expressed in breast cancer , which may play an im-portant role in the development and progression of breast cancer. 5-aza-2′-deoxycytidine, up-regulating the p53 expression in breast cancer cell line, which provides the evidents for the development of therapeutic drugs for the patients with low expression of p53 breast cancer.

Efficacy of transurenthral resection of muscle-invasive bladder cancer in the elderly / 中华老年医学杂志

Objective To investigate the clinical effects of transurenthral resection of muscleinvasive bladder tumor (TURBT) in senile. Methods This study was clinical prospective random research.46 senile cases with muscle-invasive bladder cancer treated by TURBT was divided into 2 groups:control group and observed group.The observed group was treated with TURBT combined with intravesical instillation of pirarubi 30 mg immediately after TURBT,the bladder wall around tumor was resected thoroughly.The control group underwent radical cystectomy and pelvic lymph node dissection. Results The clinical improvement rate was higher in observed group than control group (85.7％ s.63.6％,X2 =5.3008,P=0.021).There was not difference in 3 years recurrence rate between observed group(25.0％) and control group(13.6％) (x2 =S.3008,P＞0.05).The 1 year survival rate were 95.8％,90.9％ and 3 years survival rate were 79.2％,72.7％ in observed group and control group,respectively,and there were no differences between the two groups(X2 =0.262,P=0.609). Conclusions TURBT combined with intravesical instillation of pirarubi can enhance clinical effect and reduce recurrence rate in the treatment of the elderly with muscle-invasive bladder cancer.

The value about the combined application of transbronchiai needle aspiration and ring clamp biopsy, also brush biopsy through bronchofibroscope on the lung tumor / 国际肿瘤学杂志

Objective To study the application value of cytological examination and biopsy for the transbronchial needle aspiration (TBNA) in the diagnosis of cancer. Methods 101 patients with lung disease by CT that need TBNA examination to make final diagnosis were selected. They were executed with routine ex-amination of TBNA. And then pathologic biopsy and cytological examination were applied on the collection sam-pies. Results 86 patients among the 96 subjects were finally diagnosed by TBNA. So the ratio of final diagno-sis was 94. 5%. And the positive ratios about transbronchial needle aspiration and ring clamp biopsy, also brush biopsy, were 71.4% 、75.8%、 61.5%. Only 5 patients were histodiagnosed by just transbrenchial needle aspiration. Conclusion TBNA plays an important role on the diagnosis of lung tumor with different types. And the combination of three methods can improve the diagnosis pro-gressively.

Efficacy Observation of Zhuanggu Huoxue Recipe for Necrosis of Femoral Head in Patients with Osteoporotic Femoral Neck Fracture / 中国药房

OBJECTIVE:To investigate the clinical efficacy of Zhuanggu huoxue recipe for necrosis of femoral head in patients with osteoporotic femoral neck fracture. METHODS:305 patients with osteoporotic femoral neck fracture randomized into therapy group and control group. Both groups were given internal fixation therapy and continuous traction treatment for skin. Therapy group were given Zhuanggu huoxue decoction (p.o. b.i.d) for 12 weeks. Control group were given ossotide and puerarin. At 1st month,2nd month,4th month,6th month,12th month,18th month after operation,roentgenograms of bone in femoral lateral position were taken to observe the fracture healing and the occurrence of femoral head necrosis. At 4th month,6th month,8th month after radiotherapy,MRI of double lateral hip was taken to observe the occurrence of femoral head necrosis. RESULTS:The rate of femoral head necrosis and nonunion of fracture in therapy group were all lower than in control group (P