Construction of corneal neovascularization rabbit model by suture / 解剖学报

Objective To compare the effect of different sutures and suture method on corneal neovascularization ( CNV) in rabbit models. Methods NV was induced by placing sutures at the corneal periphery of rabbits (n = 45). To observe the NV status, 45 rabbits were randomly divided into 5 equal groups. Group A applied 8-0 absorbable suture (A1 single loop parallel suture, A2 single loop vertical suture). In group B, 10-0 nylon suture was used (B1 double loop parallel suture, B2 double loop vertical suture, B3 three loop radial suture). The development of CNV was observed with slit lamp microscope and photographed. Therefore the effective model for neovascularization induction was selected. Histological examination, immunofluorescent staining and ELISA analysis for the vascular endothelial growth factor( VEGF) were performed before suture, 7 and 14 days after suture. Results Sutures fell off and CNV gradually atrophied in group Al and A2; At the 14th day after suture, Sparse or short cluster CNV grew into the corneal margin in group B1 and B2, while CNV was vigorous and grew in bundles in group B3. The expression of VEGF in aqueous humor increased in B3 group after suturing, and increased in 14 days as compared with 7 days after suture. Corneal edema, neovascularization and little immunofluorescence staining for VEGF were detected in group B3 after 7 days suture. More neovascularization and immunofluorescence staining for VEGF were detected in group B3 after 14 days suture. Conclusion Corneal NV can be induced successfully in rabbit model by suturing. The method of 10-0 thread with three sets of circular seams (B3) is stable and effective.

Chinese Expert's Perspectives on Andrological Diseases Responding Specifically to Traditional Chinese Medicine / 中国实验方剂学杂志

In recent years， the incidence rate of andrological diseases has shown a significant growth trend. Considering the unavailability of a perfect theoretical system for andrology in traditional Chinese medicine （TCM） and the complex pathogenesis despite of the limited types of andrological diseases， it is necessary to improve the clinical efficacy of andrological diseases so as to satisfy the needs of patients. Therefore， the China Association of Chinese Medicine （CACM） organized the andrologists of TCM and western medicine and the outstanding young clinicians to discuss the andrological diseases responding specifically to TCM or integrated TCM and western medicine， such as chronic prostatitis， male infertility， benign prostatic hyperplasia， erectile dysfunction， and premature ejaculation， determine their diagnostic criteria in western medicine， and standardize the specifications for TCM diagnosis and treatment based on syndrome differentiation， thus formulating recognized and integrated diagnosis and treatment protocols. Apart from proposing suggestions on the treatment of such andrological diseases with TCM and western medicine， the experts have also figured out the andrological diseases responding specifically to TCM， the optimal intervention time of TCM and western medicine， and the suitable measures including surgery. The resulting consensus helps to better guide the formulation of accurate， personalized， and optimized treatment plans in clinical practice and improve the diagnosis and treatment effects of andrological diseases by giving full play to the advantages of TCM， which will in turn contribute to further innovation and development of TCM.

Expert Consensus on Otorhinolaryngology Head and Neck Diseases Responding Specifically to Traditional Chinese Medicine / 中国实验方剂学杂志

Traditional Chinese medicine （TCM） and western medicine have their respective advantages and limitations in the diagnosis and treatment of common otorhinolaryngology head and neck diseases. Although the integrated TCM and western medicine exhibits definite curative effects， there is no consensus on the otorhinolaryngology head and neck diseases responding specifically to TCM or integrated TCM and western medicine， as well as the diagnosis and treatment schemes. The China Association of Chinese Medicine （CACM） thus organized the otorhinolaryngology head and neck specialists of both TCM and western medicine to discuss the etiology， pathogenesis， and clinical diagnosis and treatment methods of common otorhinolaryngology head and neck diseases with the results of multiple clinical trials taken into account. The acute pharyngitis， chronic pharyngolaryngitis， paraesthesia pharyngis， hysterical aphasia， allergic rhinitis， subjective tinnitus， and otogenic vertigo were confirmed to respond specifically to TCM or integrated TCM and western medicine. Then a mutually agreed diagnosis and treatment scheme and recommendation with integrated TCM and western medicine was formulated as a reference for clinical practice， thus benefiting more patients.

Pharmacokinetic characteristics of 14CCHMFL-FLT3-122 in rats / 药学学报

The study was conducted to characterize the pharmacokinetics, distribution, metabolism and excretion of CHMFL-FLT3-122 after a single oral dose of 50 mg·kg-1 [14C] labeled CHMFL-FLT3-122 in rats. Isotope tracing techniques were used to analyze drug concentration and identify the distribution of drugs in tissues and metabolites in biological samples. The experiments were approved by the Animal Ethics Committee of XenoBiotic Laboratories-China, Inc. The absolute bioavailability in male and female rats were 45.83% and 50.92% respectively. The parent drug and its metabolites were extensively distributed in the stomach, intestine, liver and lung, and were eliminated completely in 48 h. The majority of radioactivity was excreted through the feces at 92.34% of the dose with a small fraction through urine at 3.99% of the dose. The parent drug was the most significant circulating component, representing 49.23% and 70.65% over the 0-48 h collection time interval in the plasma of male and female. Two major metabolites, M553 (sulfate conjugate) and M457 (N-dealkyl product), were identified in plasma. Metabolites of CHMFL-FLT3-122, including ten phase I and four phase II metabolites, were identified. The metabolic pathways of CHMFL-FLT3-122 were proposed as N-dealkylation, oxidation, amide hydrolysis, sulfate conjugation, and glucuronic conjugation.

Nano-hydroxyapatite/chitosan/alginate for repairing mandibular defects / 中国组织工程研究

BACKGROUND:Studies have confirmed that the nano-hydroxyapatite/chitosan/alginate composite materials have a certain flexibility and strength and possess a bioactivity similar to human bone. OBJECTIVE:To explore the effect of the nano-hydroxyapatite/chitosan/alginate composite materials on the repair of rabbit mandible defects. METHODS:Bilateral mandibular defect models of 10 mm × 5 mm × 5 mm were made in 18 healthy New Zealand white rabbits. Then, the rabbits were divided into two groups:experimental group was implanted with nano-hydroxyapatite/chitosan/alginate composite material, and control group was implanted with hydroxyapatite/chitosan composite. At 4, 8 and 12 weeks after implantation, cone-beam CT was applied to observe implant degradation, cal us growth and bone connection in the defect area;new bone formation was observed by hematoxylin-eosin staining. RESULTS AND CONCLUSION:The gray values of the bone density in the experimental group and control group gradual y increased with time, and there were remarkably significant differences between the two groups at different time points (P<0.01). At the same time point, the experimental group was superior to the control group in gross observation, cone-beam CT observation, gray value of CT and histological observation (P<0.05). At 4-8 weeks after implantation, the implant materials in the two groups were gradual y degraded with a blurred junction between the defect and bone tissue, and a smal amount of new bone formed tightly integrated to the recipient bone tissue, in which the experimental group was more significant. And during 8 to 12 weeks, the degradation of implanted materials in the two groups was basical y complete, and the implant began to merge with the recipient bone tissue, with further generation of new bone tissue and gradual repair of bone defect area, in which, the experimental group was more obvious. Results show that the nano-hydroxyapatite/chitosan/alginate can repair bone defects effectively, and promote the new bone formation.

The effect of adipose-derived stem cells on viability of random pattern skin flap in rabbits / 中华整形外科杂志

<p><b>OBJECTIVE</b>To explore the effect of the adipose derived stem cells (ADSCs) on the survival of random pattern skin flap in rabbits.</p><p><b>METHODS</b>ADSCs were isolated from fresh human fat and expanded in vitro for five passages. Then the characterization of ADSCs were determined by their CD marker profile and their ability to differentiate into osteogenic, adipogenic and chondrogenic lineages. On the back of the rabbits, two symmetric cephalic-based random pattern skin flaps were designed (6 cm x 2 cm). The right flaps were used as experimental groups with the contra-side flaps as control group. Human ADSCs were pre-labeled before seeding with fluorescent 3, 30-dioctadecyloxacarbocyanine perchlorate (DiO) dye. The experimental flaps evenly received 5-point injection of 2 x 10(6)Dio-labeled ADSCs resuspended in 0.5 ml of serum-free DMEM, while only 0.5 ml medium was injected into the control flaps. Seven days later, the survival rate of flaps was evaluated. The flaps underwent frozen section and were observed under the laser scanning confocal microscope to detect the fluorescence imaging. Flaps also underwent HE staining and were observed under light microscope to detect the vascular density.</p><p><b>RESULTS</b>Compared with control group, there was a significant increase of flap survival rate in the experimental group (P < 0.01). Histological analysis also demonstrated a statistically significant increase in capillary density in the experimental group.</p><p><b>CONCLUSIONS</b>It suggests that ADSCs have a better immune compatibility and potential for enhancing the blood supply of random pattern skin flaps.</p>

Effect of abdominal acupuncture on pain of pelvic cavity in patients with endometriosis / 中国针灸

<p><b>OBJECTIVE</b>To compare the effect of abdominal acupuncture and Chinese medicine on pain relieving in pelvic cavity in patients with endometriosis.</p><p><b>METHODS</b>Fifty-eight cases were randomly divided into 2 groups. Thirty cases were in abdominal acupuncture group and 28 cases in Chinese medicine group. Abdominal acupuncture points such as Zhongwan (CV 12), Xiawan (CV 10) and Qihai (CV 6), etc. were adopted for the abdominal acupuncture group, and Tianqi Tongjing Capsule (radix notoginseng capsule for dysmenorrhea) was taken by the Chinese medicine group. After a 3-month treatment, the scores of McGill pain questionaire, level of serum CA125, average value of the radial line of endometrial cyst of ovary and the sum of 3 radial lines of the uterus of patients with adenomyosis as the complication of both groups were observed before and after treatment.</p><p><b>RESULTS</b>The McGill estimation of 6 items for both groups improved obviously after treatment (all P < 0.01, except numbers of selected deseriptors in Chinese medicine group). The differences of the result of McGill estimation of 6 items after treatment had statistical significance, the scores in the abdominal acupuncture group were obviously better than those in the Chinese medicine group (all P < 0.01). The differences of CA125 levels within one group or between 2 groups had statistical significance (P < 0.01, P < 0.05). The difference of the radial lines of patients with endometrial cyst of ovary within one group or between 2 groups after treatment had not statistical significance (all P > 0.05). For the value of 3 radial lines of the uterus of patients with adenomyosis within one group before and after treatment, only the difference in the abdominal acupuncture group had statistical significance (P < 0.01). The differences before and after treatment in the Chinese medicine group and the difference between 2 groups after treatment had no statistical significance (all P > 0.05).</p><p><b>CONCLUSION</b>Effect of abdominal acupuncture on relieving pain of pelvic cavity caused by endometriosis, reducing the level of serum CA125 is obverious than Tianqi Tongjing Capsule (radix notoginseng capsule for dysmenorrhea). However, the effects on reducing the size of the ovarian endometrial cyst and the size of uterus with adenomyosis are not significant. Therefore, it is concluded that abdominal acupuncture is a better choice for endometriosis with pain as the chief complaints.</p>

Biomass structure analysis of Atractylodes lancea in different ecological environments / 中国中药杂志

<p><b>OBJECTIVE</b>To study the ecological environments of Atractylodes lancea by biomass structural analysis.</p><p><b>METHOD</b>Through the scientific investigation in Maoshan, the sampling spots were set up, the relation between growth and ecological environments was researched and the ecological environments of A. lancea were divided as following: the vegetation layer, the shrub layer, the shrub-weed layer and the weed layer. The ramet biomass, height, leaves and coverage of A. lancea were studied.</p><p><b>RESULT</b>The several factors (ramet biomass, height, leaves and coverage) showed the regular change. Among maximum, minimum and average, the shrub layer was the biggest, the shrub and weed layer was the second biggest and the vegetation layer and the weed layer was the least.</p><p><b>CONCLUSION</b>A. lancea tends to distribute in the shrub layer and the shrub-weed layer.</p>

Study on climate regionalization of Artemisia annuua in Guangxi / 中国中药杂志

<p><b>OBJECTIVE</b>To analyze the major effective factors under the climatic conditions of Guangxi which influence artemisinin content, in order to determine the best planting region.</p><p><b>METHOD</b>The correlation, the gradually regression analysis with the statistical analysis system, the geography space analysis and the regionalization with GIS were used for the study.</p><p><b>RESULT AND CONCLUSION</b>The temperature and the sunshine-hour were the major effective factors to artemisinin content, followed by the rainfall amount, the humidity showed less influence, and wind speed had no effect; And the climatic factors of seedling stage and the flowering season were the most influences to the artemisinin content. The artemisinin content was higher during the flowering season, in the region of temperature relatively lower and the rainfall amount smaller. The knoll and the mountainous region in northeast and southwest of Guangxi is the best suitable region for the Artemisia annua planting. The plain area in the southeast and middle of Guangxi is the not suitable region; Other areas are suitable regions for the A. annua planting.</p>

Comparing of different methods on habitat adaptive division of Chinese material medica / 中国中药杂志

<p><b>OBJECTIVE</b>To comparing two kinds habitat adaptive division of Chinese material medica with different models.</p><p><b>METHOD</b>The habitat adaptive divisions of A. lancea according essential oil accumulation with two kinds pattern, model pattern and template pattern were carrid and compared.</p><p><b>RESULT</b>Two habitat adaptive divisions of A. lancea maps according essential oil accumulation were gotten.</p><p><b>CONCLUSION</b>Both model pattern and template pattern were efficient on habitat adaptive division of Chinese material medica, but they shoud bu used with different processesd and based different background [corrected]</p>

Remote sensing resource monitoring on Atractylodes lancea / 中国中药杂志

Remote sensing technology was used for investigation of the resources of Atractylodes lancea. Firstly, the general situation of Jiangshu Maoshan and A. lancea in Maoshan was introduced; Secondly, the methods of remote sensing on the resource of the wild drugs were explained. Thirdly, the TM images were interpret according to the differences of the objects reflex spectrum, and growth environments in Damao mountain, Ermao mountain and Xiaomao mountain were divided into different sub-areas according to the results of the field investigations. Finally, the resource of A. lancea in Jiangshu Maoshan was estimated.

Statistical analysis of residues of heavy metals in Chinese crude drugs / 中国中药杂志

<p><b>OBJECTIVE</b>To analyze the residues of heavy metals in Chinese crude drugs.</p><p><b>METHOD</b>The SPSS 13.0 software was used to analyze the data of Cu, Pb, As, Cd, Hg collected in literatures. And, Green Trade Standards of Importing & Exporting Medicinal Plants & Preparations was used as the standard to evaluate the pollution condition of Cu, Pb, As, Cd, Hg in Chinese crude drugs.</p><p><b>RESULT</b>Chinese crude drugs were contaminated by heavy metals in different levels. The content of Cu, Pb, As, Cd, Hg exceeded the limit of the standard and the percentage was 21.0%, 12.0%, 9.7%, 28.5%, 6.9%, respectively; the phenomena of two, three and four metals exceeding limited standard simultaneity in a drug were also found and the percentage was 4.6%, 1.5%, 0.7%, respectively; the content of heavy metals in Radix Platycodi, Radix Asari and Rhizoma Coptidis was higher among the thirty-six Chinese crude drugs; All of the content of five heavy metals in Barbary Fructus Lycii Rhizoma Anemones Raddeanae, Radix Panacis Quiquefolii and Fructus Aurantii were below the limit; The pollution levels of heavy metals in different locality were different; The content of Cu, Pb in cultivated Chinese crude drugs was higher than that in wild Chinese crude drugs, while the content of As in wild Chinese crude drugs was higher than that in cultivated Chinese crude drugs.</p><p><b>CONCLUSION</b>The pollution levels of heavy metals in Chinese crude drugs were detailed reported in this paper. And it provided ways for the further study of heavy metals in Chinese crude drugs.</p>

Application of multivariate statistical methods in research of Chinese medicinal materials resource ecology / 中国中药杂志

In this paper the application of multivariate statistical methods in research of Chinese medicinal materials resource ecology was introduced. The importance of multivariate statistical analysis used in research on analyzing ecological environment factors and region suitability of Chinese crude drugs has been pointed out. Several frequent used multivariate statistical methods have been presented.

Plant hydroponics and its application prospect in medicinal plants study / 中国中药杂志

This article introduced the theorem and method of hydroponics. Some examples of studies in agriculture and forestry were presented, the effects of elements, environmental stress and hormones on physiology of medicinal plants by using hydroponics were analyzed. It also introduced the feasibility and advantage of hydroponics in intermediate propagation and allelopathy of medicinal plant. And finally it made the conclusion that the way of hydroponics would be widely used in medicinal plant study.

Study on Modified Xiaoyao Decoction combining Clomipramine treating depression / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To observe the efficacy of Modified Xiaoyao Decoction (MXD) combining clomipramine in treatment of depression.</p><p><b>METHODS</b>One hundred and five depression patients were randomly assigned to two groups. The treated group (53 patients), all were differentiated as Gan-qi stagnation syndrome type and treated with MXD combining clomipramine 25-50 mg/d. The control group (52 patients) treated with clomipramine 75-225 mg/d alone. The treatment lasted for 8 weeks in both groups. Scores of depression was assessed with Hamilton Depression Scale (HAMD) before treatment and at the 2nd, 4th, 8th week of the treatment, efficacy and adverse reactions were evaluated with Global Impression of Change (CGI) and Treatment Emergent Symptom Scale (TESS) respectively.</p><p><b>RESULTS</b>After treatment the scores in both groups were significantly different from those before treatment (P < 0.01), but no significant difference was found between the two groups (P > 0.05). Main adverse reactions were dry mouth, constipation in the treated group, much less than those in the control group, which were mainly dry mouth, constipation, dizziness, insomnia, blurred vision and prolonged Q-T interval (P < 0.01).</p><p><b>CONCLUSION</b>MXD combining clomipramine has the efficacy on depression similar to clomipramine, but has less adverse reactions.</p>

Study on dynamical changes of Ginkgo biloba resources in Pizhou city of Jiangsu Province base on RS and GIS / 中国中药杂志

<p><b>OBJECTIVE</b>To analyse the dynamical changes of the Ginkgo biloba's resources from 2001 to 2006, in Pizhou city, Jiangshu province by useing spatial analytical function of GIS and RS technology.</p><p><b>METHOD</b>Use the GIS and RS technology, extracted the information of G. biloba by scientific investigation, researched the spatial distribution and dynamical changes of G. biloba based on landsat 5 TM: the Apr. 3rd, 2001; Jan. 16th, 2005; July 30th, 2006.</p><p><b>RESULT</b>Ginkgo biloba's resource was 1.61 x 10(5) hm2 in 2001, 1.84 x 10(5) hm2 in 2005, 1.88 x 10(5) hm2 in 2006.</p><p><b>CONCLUSION</b>Ginkgo biloba's resource rised from 1.61 x 10(5) hm2 to 1.88 x 10(5) hm2 from 2001 to 2006, showed the gradually rise.</p>

Remote sensing monitoring methods for medicinal plants resource in different types of ecological environment / 中国中药杂志

Different remote sensing monitoring methods are needed for the medicinal plant resource in different types of ecological environment. This paper explained remote sensing monitoring methods for the resource of the wild medicinal plants and cultivated plants, and analyzed the rare species, generous species and species in special ecological environment in detail. It provides a new method to all kind of medicinal plants resources' remote sensing monitoring.

Screening of Anaerobic Fungi and their Medium Modification for Xylanase Production / 微生物学通报

Twelve anaerobic fungal strains isolated from rumen and faeces of ruminants were screened for xylanase prodution. Isolate A4 strain identified as Neocallimastix had the highest xylanase activity among all isolates. With rice straw, corn straw , peanut straw and filter paper as fermentation substrates, the activities of xylanase by A4 were14.31 U/mL, 11.39 U/mL, 6.99 U/mL, 13.38 U/mL, respectively. The effect of cell-free rumen fluid and yeast extract on xylanase production was tested. The results showed that the concentration level of cell-free rumen fluid had no significant effect on xylanase production. However as yeast extract concentration decreased from 1.0 g/L to 0,5 g/L , the enzyme activity decreased significantly ( P