Preliminary study on Zhejiang provincial standard of laboratory zebrafish breeding / 中国比较医学杂志

Because of its unique advantages , zebrafish has been more and more widely used in drug screening and safety assessment , environmental protection , developmental biology and other fields . But the standardization of environments, feed, and water environment of zebrafish are prominent problems and weakness which hindered their application .This paper probes into the standardization of environment and feed of zebrafish in Zhejiang , in order to promote its standardization construction .

Observation of Reproductive Capacity and Growth on 44th Generation Z:ZCLA Mongolian Gerbil / 医学研究杂志

Objective To observe Mongolian gerbil's reproducing performance and growth indexes of biology for guidance of development and use of laboratory anima1. Methods 200 gerbils produced by seed-parents with good reproductive capacity and body constitution were selected (females and males were 100 each).When 2.5~3.0 months old,one male and one female were cohabited randomly for a long period of time.Results Every embryo gave birth to 14 young gerbils at best and 1 gerbil at least.The total embryos was 455 and the total young gerbils was 3191. The average young gerbils of every embryo were 7.01,and every embryo gave mostly birth to 5~9 young gerbils,accounting for 79.34 percent of all the embryos. The shortest time between two embryos was 20 days and the longest time was 127 days,and mostly were 20~60 days,accounting for 72.16 percent of total. Conclusion The body weight,body length and tail length of Mongolian gerbils grow rapidly from birth to 4 months old. The differences between female and male was not significantly before delectation,and the in the growing time after delectation,the body weight,body length and tail length of male gerbils were all greater than those of female gerbils.

Changes of Antioxidant Activities on Z:ZCLA Mongolian Gerbils' Sera within Different Growth Period / 医学研究杂志

Objective To study the changes of antioxidant on ZCLA Mongolian gerbils' sera within different growth period.Methods The gerbils at the age of 1 month,3 months and 24 months were used in the experiment,with each 16(half male and half female),and the sera were collected for determining the MDA,SOD and GSH-Px.Results At the age of 3 months,MDA was the lowest,but GSH-Px was the highest,while the SOD was ascending with growing.Conclusion The changes of GSH-Px showed the direct relation to MDA,while the changes of the SOD was irrespective to the changes of MDA.

Skin Toxicity Effects of Common Disinfectants of Barrier Environment on Laboratory Animals / 医学研究杂志

Objective To observe the toxic reaction caused by short time contact with some common disinfectants such as 0.2% sodium hypochlorite,2%acetic acid and 2% glutaraldehyde and0.2%acetic acid,through wounded skin of laboratory animals and laborers.Methods Toxicity experiments of these disinfectants by artificial skin wound were carried on in guinea pigs,and the poisoning performance and the death situation of guinea pigs were observed.Results Light edema was caused by using 2% acetic acid,and the symptom disappeared after 3 days;it appeared the symptom of red bump and hemorrhagic spots after use 2% glutaraldehyde,and ecdysis occured after 4days,the red bump disappeared after 5 days with a brownish discoloration left;it didn't appear any toxic reaction by using 0.2% sodium hypochlorite and 0.2%acetic acid.Conclusion There was toxic effect of 2% acetic acid and 2% glutaraldehyde to animals and laborers.