RESUMO
OBJECTIVE@#Previous studies have indicated that the plasticizer di (2-ethylhexyl) phthalate (DEHP) affects lipid accumulation; however, its underlying mechanism remains unclear. We aim to clarify the effect of DEHP on lipid metabolism and the role of TYK2/STAT1 and autophagy.@*METHODS@#In total, 160 Wistar rats were exposed to DEHP [0, 5, 50, 500 mg/(kg•d)] for 8 weeks. Lipid levels, as well as mRNA and protein levels of TYK2, STAT1, PPARγ, AOX, FAS, LPL, and LC3 were detected.@*RESULTS@#The results indicate that DEHP exposure may lead to increased weight gain and altered serum lipids. We observed that DEHP exposure affected liver parenchyma and increased the volume or number of fat cells. In adipose tissue, decreased TYK2 and STAT1 promoted the expression of PPARγ and FAS. The mRNA and protein expression of LC3 in 50 and 500 mg/(kg•d) groups was increased significantly. In the liver, TYK2 and STAT1 increased compensatorily; however, the expression of FAS and AOX increased, while LPL expression decreased. Joint exposure to both a high-fat diet and DEHP led to complete disorder of lipid metabolism.@*CONCLUSION@#It is suggested that DEHP induces lipid metabolism disorder by regulating TYK2/STAT1. Autophagy may play a potential role in this process as well. High-fat diet, in combination with DEHP exposure, may jointly have an effect on lipid metabolism disorder.
Assuntos
Animais , Feminino , Masculino , Tecido Adiposo , Metabolismo , Autofagia , Peso Corporal , Dieta Hiperlipídica , Dietilexilftalato , Toxicidade , Disruptores Endócrinos , Toxicidade , Metabolismo dos Lipídeos , Transtornos do Metabolismo dos Lipídeos , Fígado , Metabolismo , Ratos Wistar , Fator de Transcrição STAT1 , Metabolismo , TYK2 Quinase , MetabolismoRESUMO
Microbial production of 5-aminolevulinic acid (5-ALA) by photosynthetic bacteria compared to other bacteria and algae was reviewed. During anaerobic cultivation of the Rhodobacter spaeroides mutant strain, control of the redox potential was effective or producing large amounts of extracellular ALA. ALA has been practically applied in agriculture as herbicide, pesticide and growth-promoting factor for plants. New agriculture applications including salt tolerance and cold temperature tolerance of plants were also desired. Finally recent medical application for cancer treatment, tumor diagnosis and other clinical uses were discussed.
RESUMO
SO2 is one of the main pollutants in the air. Tradational disposal methods are limited in the industrial application owing to their high cost. Some foreign scholars began to research in microbial treatment of SO2 both in laboratory test and in full-scale test in recent years and good results were achieved. This method can significantly lowering costs and acquire sulfur product and microbial biomass protein of high quality. The microbial method which is simple, effect and of low costs can satisfy needs of industry and give attention to environmental, economical and social benefits, therefore, it will have amplitude and well application prospects.