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Objective@#To understand current status of children s first permanent molar in Harbin, to investigate parental awareness regarding children s first permanent molar as well as Pit and Fissure sealant, and to provide new ideas for caries prevention in the first permanent molar and Pit and Fissure sealant.@*Methods@#In October 2019, 11 540 children in the region were examined and their parents were given questionnaires.@*Results@#The prevalence of dental caries was 37.72%. The DMFT was 1.11, the germination rate was 86.98%, and Pit and Fissure sealants rate was 36.93%. About 16.8% of the parents were aware of the eruption time of first permanent molar, and 35.33% didn t know first permanent molar, 19.39% of the parents had a clear understanding of Pit and Fissure sealants time and 32.77% of the parents were not aware of Pit and Fissure sealants. The prevalence of caries was higher in children (35.55%, 32.77%) whose parents did not know the first permanent molar and the pit and fissure sealants.High income level, high education level and urban parents had a higher degree of knowledge about fossa closure( χ 2=98.35, 192.16, 172.31, P <0.05).@*Conclusion@#Prevalence of dental caries is higher in children in Harbin, and the awareness of Pit and Fissure sealants is lower in parents. Relevant organizations should strengthen the publicity and education of oral health knowledge for parents.
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Aim To observe the effects of sacubitril/valsartan (Sac/Val, LCZ696) on atrial remodeling and atrial fibrillation (AF) susceptibility in spontaneously hypertensive rats (SHR). Methods Twenty-four 7-week-old male SHR were randomly divided into SHR group, SHR + Val group (30 mg · kg
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Objective: To investigate whether inflammatory factor tumor necrosis factor-α (TNF-α) is involved in the electrical remodeling of cardiomyocytes by regulating ultra-rapid delayed rectifier K(+) current (I(kur)) and the role of Src kinase. Methods: H9c2 cells, embryonic cardiomyocytes of rat, were cultured in Dulbecco's modified Eagle's medium (DMEM) and atrium-derived HL-1 cells were cultured in Claycomb medium. Both H9c2 and HL-1 cells were cultured at 37 ℃ with 5% CO(2). Cells cultured in normal conditions without additional treatment served as control group. Experimental groups were treated with different concentration of TNF-α (25 or 50 or 100 ng/ml) for 24 hours. To study whether Src specific inhibitor PP1 could abrogate the effect of TNF-α, cells were pre-treated with 10 μmol/L PP1 for 1 hour, followed by TNF-α (100 ng/ml) for 24 hours. Western blot and the whole cell patch clamp technique were used to detect the protein expression of Kv1.5 and Src and I(kur) in each group. Results: (1) In H9c2 cells, high concentration of TNF-α treatment (100 ng/ml) significantly reduced the Kv1.5 protein expression compared with control group and TNF-α 25 ng/ml group (both P<0.05). Compared with control group, the expression of p-Src protein was higher in 25 ng/ml, 50 ng/ml, 100 ng/ml TNF-α group (all P<0.05), but there was no statistical difference in the expression of Src protein among groups (P>0.05). In addition, the current density of I(kur) was decreased in 50 ng/ml, 100 ng/ml TNF-α group (both P<0.05). Furthermore, the expression of Kv1.5 protein and the current density of I(kur) were increased in PP1+TNF-α group compared with TNF-α 100 ng/ml group (both P<0.05). There was no statistical difference in the expression of Kv1.5 protein and the current density of I(kur) between the control group and PP1+TNF-α group (both P>0.05). (2) In atrium-derived HL-1 cells, the expression of Kv1.5 protein was reduced in 100 ng/ml TNF-α group compared with control group and TNF-α 25 ng/ml group (both P<0.01). In addition, the expression of p-Src protein was increased in TNF-α 100 ng/ml group compared with control group (P<0.05), but there was no statistical difference in the protein expression of Src among groups (P>0.05). The expression of Kv1.5 protein was increased in PP1+TNF-α group compared with TNF-α 100 ng/ml group (P<0.05). Conclusion: TNF-α is involved in the pathogenesis of atrial fibrillation, probably via decreasing I(kur) current density in atrium-derived myocytes through the activation of Src kinase.
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Animais , Ratos , Regulação para Baixo , Átrios do Coração , Miócitos Cardíacos , Fator de Necrose Tumoral alfa , Quinases da Família srcRESUMO
<p><b>OBJECTIVE</b>To study the sensitivity of Jatropha curcas seeds from three different locations to (60)Co-gamma radiation and to determine the medial lethal doses (LD50) of (60)Co-gamma radiation for these seeds.</p><p><b>METHODS</b>Six different radiation doses (0, 100, 150, 200, 250 and 300 Gy) were used. Based on the germination rate 50%, LD50 doses of (60)Co-gamma radiation for the seeds were calculated using linear regression equation.</p><p><b>RESULTS</b>LD50 doses of (60)Co-gamma radiation for these seeds were 178 Gy (seeds from Guangdong), 132 Gy (seeds from Hainan) and 198 Gy (seeds from India) respectively. Increasing radiation doses caused more significant changes in leaf shape of the M1 seedlings.</p><p><b>CONCLUSION</b>The results provides an important experimental basis for the radiation breeding of the important herbal and energy plant J. curcas.</p>
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Radioisótopos de Cobalto , Toxicidade , Raios gama , Germinação , Efeitos da Radiação , Jatropha , Efeitos da Radiação , Dose Letal Mediana , Sementes , Efeitos da RadiaçãoRESUMO
<p><b>OBJECTIVE</b>To evaluate the molluscicidal activities of methanol extract of Jatropha curcas leaves against Ampullaria gigas.</p><p><b>METHODS</b>Young snails, adult snails and eggs of Ampullaria gigas were treated with the methanol extract of J. curcas leaves at different doses for different time lengths and the molluscicidal effects of the extract were evaluated.</p><p><b>RESULTS</b>The methanol extract showed a significant molluscicidal effect on the young snails at a low concentration, and treatment with 75 mg/L extract for more than 3 days resulted in a 100% mortality rate of the young snails. The Jatropha leaf methanol extract also showed toxicity to adult snails and eggs.</p><p><b>CONCLUSION</b>Jatropha leaves have a great potential for developing green pesticides to control Ampullaria gigas, but its biochemical mechanism needs further research.</p>
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Animais , Jatropha , Química , Metanol , Química , Moluscocidas , Farmacologia , Extratos Vegetais , Farmacologia , Folhas de Planta , Química , Caramujos , ClassificaçãoRESUMO
<p><b>OBJECTIVE</b>To analyze the bioactive components in Jatropha curcas leaves using gas chromatography-mass spectrometry (GC-MS).</p><p><b>METHODS</b>The bioactive components were extracted from J. curcas leaves by supercritical fluid CO2 extraction and analyzed by using GC-MS.</p><p><b>RESULTS</b>Seventy peaks were detected by GC-MS, and 43 compounds were identified (61.43%). Among the identified compounds, 16 had a content of more than 1%, and the total contents of these 16 compounds reached 81.36%. The four most abundant components were 22,23-dihydro-stigmasterol (16.14%), alpha-tocopherol (15.18%), beta-amylin (7.73%) and dotriacontanol (7.02%). The content of gamma-tocopherol reached 2.88% and vitamin E reached 18.06% in the extract.</p><p><b>CONCLUSION</b>J. curcas leaves contain multiple compounds with anti-tumor, anti-virus and antimicrobial activities.</p>
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Cromatografia com Fluido Supercrítico , Métodos , Cromatografia Gasosa-Espectrometria de Massas , Jatropha , Química , Extratos Vegetais , Folhas de Planta , QuímicaRESUMO
<p><b>OBJECTIVE</b>To compare the oil contents and fatty acid composition among the samples of Jatropha curcas L. seeds collected from China (Guangdong, Hainan, and Guizhou Provinces) and India.</p><p><b>METHODS</b>Soxhlet extraction method and gas chromatography-mass spectrometry (GC-MS) were employed to determine the oil contents of Jatropha seeds and the fatty acid composition of Jatropha oil.</p><p><b>RESULTS</b>The seed oil contents (dry basis) were 32.43% (Guangdong), 31.41% (Hainan), 37.56% (Guizhou) and 41.04% (India), respectively. Twelve different fatty acids were detected by GC-MS, and the content of total unsaturated fatty acids accounted for 80.93%, 79.53%, 77.24% and 78.22% of the total fatty acids in the samples collected from Guangdong, Hainan, Guizhou and India, respectively.</p><p><b>CONCLUSION</b>There are differences in the oil contents and fatty acid composition among the J. curcas seeds collected from different regions, and attention should be given to these differences in the introduction and breeding of J. curcas.</p>
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China , Ácidos Graxos , Cromatografia Gasosa-Espectrometria de Massas , Índia , Jatropha , Química , Classificação , Óleos de Plantas , Sementes , Química , Especificidade da EspécieRESUMO
<p><b>OBJECTIVE</b>To develop a new technique for efficient and rapid non-test tube cloning of the medicinal and energy- producing plant Jatropha curcas.</p><p><b>METHODS</b>Using the mini-stem fragment (2-3 cm) of Jatropha curcas with merely one axillary bud as the explant, the effect of an auxin IBA concentration on the plantlet regeneration was studied.</p><p><b>RESULTS AND CONCLUSION</b>When treated with 1 mg/LIBA for 1h, the explants showed the most rapid propagation. The mini-stem fragments high root regeneration ratio (96.7%), short root regeneration period (18.2-/+2.0 d), large number of new roots per explant (6.3-/+1.8), and long total root length (6.8-/+3.5 cm), demonstrating that this technique can be a simple and efficient method for rapid non-test tube cloning of Jatropha curcas of potential industrial value.</p>