Decorative repair of nail-bed defect at finger by partial nail-bed flap at second toe with blood-vessel anastomosed / 中华显微外科杂志

Objective To discuss the clinical effect of partial nail-bed flap at second toe with blood-vessel anastomosed in repair of different types of nail-bed defects at finger.Methods From July,2010 to July,2015,25 cases of partial nail-bed flap at second toe with blood-vessel anastomosed were treated,including 14 males and 11 females,with an age range from 18 to 45 years old (average 25 years old).Eleven cases at index finger,6 cases at middle finger,5 cases at ring finger and 3 cases at little finger.According to the classification of nail bed defect site,9 cases at lateral,5 cases at nail root and 11 cases at distal.All cases were combined skin and soft tissue defect.The area of defects were from 1.1 cm × 2.0 cm to 2.5 cm × 3.2 cm.The time from injury to admission were from 1 hour and 12 minutes to 3 hours and 12 minutes,the average time was 2.1 hours.The area of incised nail flap during operation were from 1.2 cm × 2.2 cm to 2.6 cm × 3.5 cm.The donor site was sutured directly or repaired with free skin graft.The toe length in all cases were retained.The nail shape,nail gloss,fine function of finger such as pinch,grip,grasp and restoration of the donor toes were compared in the follow-up.All patients were followed-up at regllar intervals.Results The nail-bed flap after transplantation and skin graft at donor site survived successfully,and primary healing of the wounds occured in all cases.The follow-up time were from 6 to 26 months(average 9 months).Longitudinal spine or transverse groove occurred in 3 cases at the nail-bed boundary between toe and finger,nail thickened and distal tilted occurred in 1 case,flat,smooth,no deformity nail and glossy deck occurred in others.The flap had good appearance,texture and elasticity.Two-point discrimination was 5-9 mm,averageal of 6 mm.The donor toe had no shortening and no pain after long-term following.According to effectiveness standard for fingernail regeneration:19 cases were excellent and 6 cases were good.Conclusion Partial nail-bed flap at second toe wih bloodvessel anastomosed in repairing nail-bed defects at finger,nail appearance is realistic and function recovered well postoperatively.

miR-149 promotes epithelial-mesenchymal transition and invasion in nasopharyngeal carcinoma cells / 中南大学学报(医学版)

Objective To investigate the function and mechanism of miR-149 in nasopharyngeal carcinoma (NPC).Methods The expression of miR-149 was examined by real-time PCR and calculated by 2-△△Ct method. The cell proliferation was analyzed by MTT assay. The cell migration and invasion were shown by the wound healing assay and transwell migration assay, and the expression of E-cadherin was detected by Western blot. Results The expression of miR-149 was higher in NPC cell lines 5-8F and 6-10B than that in normal immortalized nasopharyngeal epithelial NP69. MTT assay showed that miR-149 promoted the proliferation of NPC cell lines. The wound healing assay showed miR-149 promoted the mobility and invasion of NPC cell lines. Inhibition of miR-149 reduced the ability of NPC cell lines to proliferate and invade. miR-149 downregulated the expression of E-cadherin, whereas antagomir which mediated knockdown of miR-149 significantly upregulated the expression of E-cadherin. Conclusion miR-149 might be involved in the invasion and metastasis of NPC through regulation of epithelial-mesenchymal transition (EMT).

Multiphase Segmentation of Liver Lesion in CT Images Based on C-V Model / 医疗卫生装备

Objective To study a semi-automatic segmentation framework for liver complex lesion in CT images. Methods A multiphase level set method of image segmentation based on C-V model was proposed, which was in connection with the complex information in the pathological changes area of liver. Depending on the overall characteristics of the image, the active curve contour of C-V model stopped on the edge of objects. It was possible for the segmentation of multi-objects because multi-phase level set was drawn into. Simultaneously, the problem of overlapping and hollow caused by more level set function was avoided. Results The method behaved well in the segmentation of CT images of the liver lesion, two different area were separated. Conclusion The segmentation tests prove that the proposed segmenting method makes a good result.

Extraction of Liver Focal Lesions in Abdominal CT Image Based on Local OTSU and Zone Market Screening / 医疗卫生装备

Objective To prepare for extraction of liver focal lesion in view of its characteristics of images. Methods A region of interest in the liver region comprising the focal lesion to create a local image was selected firstly, then the threshold to pre-segment the local image was got automatically by using local OTSU in clusters algorithm, at last mark sifting methods to get red of inner holes and outer dissociate areas to get the final contour was used. Results The algorithm can efficiently extract the focal liver lesion. Conclusion The way of extraction of liver focal lesions with local OTSU and zone market screening is rapid and effective.

Technological transformation to luminous flux of DTG160 single-pan analytical balance / 医疗卫生装备

This article analyses the deficiencies of DTG160 single-pan analytical balance and elaborates the basis of its technological transformation.Luminous fluxes of 20 balances are successfully transformed.What' s more,this transformation method is also suitable for other mechanical single-pan or double-pan analytical balance.

Extracorpoveal Circulation Speed-Measuring Apparatus / 医疗卫生装备

This paper introduces a simple speed-measuring apparatus equipped with single-chip microcom- puter,MCS-51.It can be used in wide fields with excellent precision.A progressive method of time sampling is adopted for programming,i.e.transfer the time per revolution to speed (with precision of 0.001s).So the characters of MCS-51,good controlling and running ability,are fully utilized.In the time-measuring pro- gramm,two impulse signals,sampled by photo-electric transformation are wisely adopted to start and stop the counter respectively.