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AIM To establish an HPLC method for the simultaneous content determination of narirutin,naringin,hesperidin,neohesperidin,honokiol and magnolol in Liuhe Dingzhong Pills (Aurantii Fructus,Citri reticulatae Pericarpium,Magnoliae officinalis Cortex,etc.).METHODS The analysis of 75％ methanol extract of this drug was performed on a 30 ℃ thermostatic Dikma Spursil C18 column (4.6 mm ×250 mm,5 μm),with the mobile phase comprising of acetonitrile-water flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 283 nm and 294 nm.RESULTS Six constituents showed good liner relationships within their own ranges (r≥0.999 3),whose average recoveries were 92.6％-103.7％ with the RSDs of 0.89％-2.87％.The contents of naringin and neohesperidin in three batches of samples showed obvious differences.CONCLUSION We should pay attention to the unstable quality of Liuhe Dingzhong Pills due to Aurantii Fructus.
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Objective To determine the content of quercetin in Semen Hoveniae.Methods A RP-HPLC method was established.The chromatographic column was Synergi 4u Fusion-RP 80.The mobile phase was acetonitrile-0.5 %phosphoric acid(33 ∶67).The flow rate was 1.0 mL?min-1,the detection wavelength was at 360 nm,and the column temperature was 30 ℃.Results The average recovery of quercetin was 98.6 %,and RSD was 2.39 %(n=6).Conclusion The method is effective,simple and accurate,and can be used to determine the content of quercetin in Semen Hoveniae.
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AIM: To determine the content of(+)-catechin and (-)-epicatechin and berberine hydrochloride in Wangying Capsule(Rhizoma Picrorhizae,Rhizoma Coptidis,Catechu,etc.). METHODS: A RP-HPLC method was established.The chromatographic column was Diamonsil-C_(18).The mobile phase was 0.04 mol/L citric acid-N,N-dimethylformamide-tetrahydrofuran(40∶8∶2) for determining(+)-catechin and(-)-epicatechin with the flow rate of 1.0 mL/min,and the detection wavelength of 280 nm.The mobile phase was acetonitrile-water(52∶48)(including 0.34% potassium dihydrogen phosphate and 0.17% sodium dodecyl sulfate) for determining berberine hydrochloride with the flow rate of 1.0 mL/min,and the detection wavelength of 265 nm. RESULTS: The average recoveries for(+)-catechin and(-)-epicatechin and berberine hydrochloride were 100.9% and 99.9% and 97.0%,precision of the method were 2.52% and 2.53% and 2.70%(RSD,n=6),respectively. CONCLUSION: The method can be used for quantitative determination of the preparation.