Experience of Professor You Zhaoling in Application of the Lotus Medicinal Herbs for Reproductive System Diseases / 中医杂志

Lotus medicinal herbs have the effects of resolving blood stasis and stopping bleeding， invigorating the spleen and benefiting the kidneys， restoring coordination between heart and kidney， and tranquilizing the fetus. Lotus root， lotus leaf， lotus seed， Lotus plumule， Shilianzi（Nelumbo nucifera Gaertn） and other lotus herbs are used to treat various female reproductive system diseases. For example， lotus leaf tip with modified Youshi Pangxing Shuangtu Decoction （伍尤氏胖型双土汤）， lotus root section with modified Youshi Shouxing Shuangteng Decoction （尤氏瘦型双藤汤） were used for polycystic ovary syndrome； Youshi Yangchao Formula （尤氏养巢方） （containing lotus seed）， Gengnian Formula （更年方） （containing lotus plumule） for ovarian reserve dysfunction； Lotus seed - Shihu （Dendrobium nobile） as the herb-pair， lotus stamen with ginseng flowers and Sanqi Flower for thin endometrium； Shilianzi（Nelumbo nucifera Gaertn）， lotus leaf tip， lotus stamen， lotus seed， lotus room， lotus root section can be used for premature abortion， and we summarized the characteristics of clinical medication， compounding experience， drug dosage and precautions of each lotus drug in different diseases.

Effects of shoutai pills on expression of Th1/Th2 cytokine in maternal-fetal interface and pregnancy outcome / 中国中药杂志

<p><b>OBJECTIVE</b>To evaluate its mechanism of inducing the maternal-fetal immune tolerance by studying the effects of Shoutai pills on the expression of Th1/Th2 cytokine and pregnancy in maternal-fetal interface of mice with recurrent spontaneous abortion (RSA).</p><p><b>METHOD</b>The normal pregnancy and RSA model were respectively induced with CBA/J x BALB/c and CBA/J x DBA/2. The mice with RSA were randomly divided into model group and low, middle and high dose groups of Shoutai pills. The mice were killed in 14 days after administration and embryo resorption rate was counted and their decidual and placental tissues were co-cultured to detect the expressions of IL-4, IL-10, IFN-gamma and TNF-alpha with ELISA.</p><p><b>RESULT</b>The embryo resorption rate of the model group was significantly higher than the normal pregnancy, middle and high dose groups of Shoutai pills could decreased the embryo resorption rate of the mice with RSA (P < 0.05). All the doses in 3 groups of Shoutai pills could decreased the expression of IFN-gamma and TNF-alpha (P < 0. 05) and there was no obvious difference between normal pregnancy group and all groups of Shoutai pills. Middle and high doses of Shoutai pills could increased the expression of IL-4 and IL-10 (P < 0.05) and there was no obvious differences between normal pregnancy and high dose group of Shoutai pills.</p><p><b>CONCLUSION</b>The mechanism about Shoutai pills can change Th1 /Th2 cytokine towards Th2 bias, which induced the maternal-fetal immune tolerance.</p>

Influence of Kidney-notifying Compound on Decapitated Henghe Female Monkeys' NO and ET / 浙江中医药大学学报

[Objective] To discuss the mechanism of Kidney-notifying Compound preventing and treating menopause coronary heart disease.[Method] Copy 4 mature Henghe female monkeys into artificial menopause models,randomly divide them into Kidney-notifying Compound group(1) and Premarin control group(2),2 monkeys in one group.Every day make stomach perfusion of the of Compound decoction;take nitrate reductase to measure serum NO,radiation-immune method for plasm ET-1 concentration.[Result] The Compound and WM combined with estrogen Premarin all can raise NO and reduce ET level,two groups have statistical meaning in replying measurement(P

Effects of herb therapy for benefiting qi and removing blood stasis on ultrastructure of vascular endothelial cells and vascular smooth muscle cells of uterine endometrium in rabbits with copper intrauterine device / 中西医结合学报

OBJECTIVE: To observe the effects of herb therapy for benefiting qi and removing blood stasis on ultrastructure of vascular endothelial cells (VECs) and vascular smooth muscle cells (VSMCs) of uterine endometrium in rabbits with copper intrauterine device. METHODS: Fifty-six rabbits were randomly divided into seven groups, which were normal control group, sham-operation group, untreated group, indomethacin-treated group and low-, medium- and high-dose Gonghuan Zhixueling Recipe (GHZXLR)-treated groups. The rabbits in the last five groups were operated with copper IUD insertion and then orally given distilled water, indomethacin and low-, medium- and high-dose GHZXLR respectively for at least one week. Rabbits in the normal control group and sham-operation group were given distilled water orally. The ultrastructure of VECs and VSMCs of uterine endometrium in rabbits was observed by transmission electron microscopy. RESULTS: The morphological changes of VSMCs revealed intracellular edema, organelle disintegration and decrease of organelle amount, or cell atrophy and vacuolar degeneration of mitochondria in the untreated group, and the amount of collagen fibers also increased outside the VSMCs. Local interstitial edema in subendothelial substance and vacuolar degeneration of mitochondria in VECs were both observed. The ultrastructural damages to the mitochondria, Golgi bodies and myofilament of VECs and VSMCs and the intercellular substance in GHZXLR-treated groups were slighter than those in the untreated group, while these damages had no significant differences as compared with those in the indomethacin-treated group. CONCLUSION: The Chinese herb therapy for benefiting qi for removing blood stasis has the protective effect on VECs of uterine endometrium in the rabbits with copper intrauterine device. It appears to be a good treatment for menorrhagia induced by copper IUD insertion.

Effect of Fuke Zhixuexiaotong Keli on the Expression of MMP-9 and TIMP-1mRNA in Rats Model of Incomplete Puerperal Involution of Uterus / 中国中医药信息杂志

Objective To explore the mechanism of Fuke Zhixuexiaotong Keli in rats model of incomplete puerperal involution of uterus by detecting the expression of metalloproteinases-9(MMP-9) and tissue inhibitor of metalloproteinase-1(TIMP-1) in the uterine redintegration incomplete model.Methods Seventy-two SD female rats were randomly divided into 6 groups:blank group,model group,Chanfukang group,and high-,middle-,low-dose of Fuke Zhixuexiaotong Keli group.The model of incomplete puerperal involution of uterus was established,and MMP-9,TIMP-1 gene expression level in each group were detected by in situ hybridization detection of uterine tissue.Results The expression of MMP-9 and TIMP-1 could be successfully detected in each group.The expression of MMP-9 in model group was significantly higher than the control group,and the expression of TIMP-1 was lower.The mRNA of MMP-9 gene was significantly decreased in both high-,middle-and low-dose of Fuke Zhixuexiaotong Keli group and Chanfukang group(P

Study on Percolation Extraction Process of Panax Notoginseng / 中国中医药信息杂志

Objective To optimize the conditions for the percolation extraction process of Panax notoginseng(Burk.) F.H.Chen.Methods Conditions for the percolation were studied by orthogonal experimental design as guided by the contents of total notoginseng saponin and total amino acids.Results The percolate rate,concentration and quantity of alcohol had significant effects on the process.Conclusion The optimum condition for the extraction of Panax notoginseng was adding 12 times amount of 50% alcohol and percolating at a rate of 1～3 mL/(min?kg).

Effects of Shoutaiwan on the STAT Signal Transduction Pathway of CD_4~+ T Cells in Maternal-fetal Interface of Mice with Recurrent Spontaneous Abortion / 中国中医药信息杂志

Objective To study the effects of Shoutaiwan on the STAT signal transduction pathway of CD4+ T cells in maternal-fetal interface of mice with recurrent spontaneous abortion(RSA).Methods CBA/J?BALB/C mating combination and CBA/J?DBA/2 mating combination were used as normal pregnancy and RSA model respectively.Mice with RSA were randomly assigned to model group and Shoutaiwan group.After 14 days of administration,the STAT signal transduction pathway of CD4+ T cells in mice decidual and placental tissues were detected by Springbio 720 antibody microarrays.Results Shoutaiwan could increase the phosphorylation level of STAT3 and STAT6 protein in the STAT signal transduction pathway.At the same time,Shoutaiwan could decrease the phosphorylation level of STAT1 protein.Conclusion The activation of STAT3 and STAT6 signal transduction pathway might play a important role in the therapy of RSA by Shoutaiwan.

The Study on the Effects of the Components of Mongolian Milkvetch Root and Red Sage Root Compound on the Expression of Matricial Metal Protease-9 (MMP-9) mRNA on Placental Nourish Cells and the Blood Plasma Level of IL-10 in Model Pregnant Rats / 中国中医药信息杂志

Objective To explore the molecular mechanism of elevating placental blood supply by the component from Mongolian Milkurtch and red sage Root. Method Abstract the effective components from Mongolian Milkurtch and red sage Root, apply L-Arginine methy1 ester (L-NAME) to make NO production blocking model, use ELISA to check IL-10 in plasma of 18 d pragant rats, and FISH to check the expression of matricial metal protease-9 (MMP-9) mRNA. Results The plasma level of IL-10 of model group was lower than that of blank group (P 0.05). Conclusion By interving the relationship between IL-10 and MMP-9, the components of Mongolian milkvetch root and red sage root compound may affect the shift and invadation of deciduas, so as to prevent fleet embedding, help building the circulation between mother and embryo, and keep the placental blood supply.

The Key and Analyzing of DUB by Traditional and Western Medicine / 中国中医药信息杂志

Objective To explore the key way of DUB (dysfunctional uterine bleeding) by traditional and western medicine. Method Analyse the traditional medicine's unique understanding of DUB, the symptoms of DUB and the function of Chinese traditional medicine on DUB. And sum up the modern molecular pathology of DUB. Result Chinese traditional medicine has some regulations in curing DUB. And given the modern molecular biology technique, it maybe play an important part in researching the essential of the syndrome of DUB and the function regulation of Chinese traditional medicine. Conclusion DUB, which is an important disease that is in the person of “menstruation adjustment”, “blooding disease”in traditional Chinese gynecology, must be studied systematically first, so as to improve the combination of traditional medicine and western medicine in gynecology.

Effects of sanqi qiancao recipe on hemorheological parameters of rabbits with copper intrauterine device / 中西医结合学报

OBJECTIVE: To explore the mechanism of Sanqi Qiancao Recipe (SQR) in treating metrorrhagia caused by copper intrauterine device (IUD) in rabbits and to provide experimental evidence for preventing and treating this disease. METHODS: Fifty-six rabbits were randomly divided into seven groups, which were normal control group, sham-operation group, untreated group, indomethacin-treated group, low-dose SQR-treated group, medium-dose SQR-treated group and high-dose SQR-treated group. Copper IUD insertion was operated in rabbits of the last five groups. Rabbits in the last four groups were treated orally with indomethacin and low-, medium- and high-dose SQR respectively for a week. Rabbits in the untreated group, normal control group and sham-operation group were given distilled water orally. Hematocrit, blood viscosity at low, medium and high shear rate, plasma viscosity and blood sedimentation were examined after treatment. RESULTS: The hematocrit, blood viscosity at low, medium and high shear rate and plasma viscosity were higher in the untreated group than those in the normal control group with significant differences (P<0.01) while those indexes in low-, medium- and high-dose SQR-treated groups were significantly lower than those in the untreated group (P<0.05 or P<0.01). CONCLUSION: SQR can lead to a decrease in blood viscosity and improve the blood flow, which may be one of the mechanisms of SQR in treating metrorrhagia after copper IUD insertion.

Epidemiological survey of TCM syndromes distribution related to kidney deficiency in 612 patients with DUB / 中华中医药杂志

Objective:To investigate the syndromes distribution in patients with dysfunctional uterine bleeding(DUB) related to kidney deficiency, and to provide the basis for standardizing the DUB syndrome differentiation of kidney deficiency. Methods: DUB Patients with kidney deficiency were chosen and accepted epidemical survey through the questionnaire, then the frequency and constituent ratio of each syndrome were calculated. Results: In 612 cases of DUB patients with kidney deficiency, there were 102 patients with simple syndromes covered 16.67%, and 510 patients with compound syndromes covered 83.33%. In patients with simple syndromes, the common syndromes were kidney-yin deficiency(45,44.12%) and kidney-qi deficiency(45,41.18%). And the syndromes of kidney-qi deficiency and kidney-yin deficiency were often respctively complicated with liver stagnation(68,11.09%; 113,18.43%), blood defi ciency(57,9.30%; 99,16.15%) and blood stasis(57,9.30%; 99,16.15%), the syndrome of kidney-yang deficiency usually complicated with spleen-qi deficiency(75,12.23%). The syndrome of kidney-qi deficiency(186,57.23%) was commonly-seen in the patients with ovular DUB, while the syndromes of kidney-qi(146,50.69%) and kidney-yin deficiency(111,38.54%) were frequently-seen in the patients with anovular DUB. Conclusion: In clinic, the compound syndromes were commonly seen in patients with DUB due to kidney deficiency, the complicated syndromes of kidney deficiency were liver stagnation, spleen-qi deficiency, blood deficiency and blood stasis..

Effect of active compound components of SQ on MMP-1,TIMP-1,VEGF,t-PA and PAI-1 of in? ammatory endometrial cell / 中华中医药杂志

Objective :To study the effect of MMP-1,TIMP-1,VEGF and t-PA,PAI-1 in the inflammatory process of endometrium cell and the active components of SQ.Methods: ICC,ISH and WB was used to test the level of protein of MMP-1,TIMP-1,VEGF and t-PA,PAI-1 and mRNA of MMP-1,TIMP-1 and VEGF of endometrium cell in regular group,pattern group,GongXueNing group and SQ group.Result: In contrast with model group,the level of MMP-1protein and mRNA expression obviously decreased,but the level of TIMP-1 and VEGF increased in SQ group,there were signifi cant differences(P

Hemostatic and analgesic effect of Gonghuan Zhixue Tablet on mice / 中西医结合学报

OBJECTIVE: To explore the hemostatic and analgesic effect of Gonghuan Zhixue Tablet (GHZXT) on mice and to produce experimental evidence for exploiting new drug for endometrorrhagia caused by Cu-intrauterine contraceptive device (Cu-IUD). METHODS: Compared with 6-aminocaproic acid and notoginseng, the effects of GHZXT on clotting and bleeding time of mice with capillary method and severed tail were investigated; and compared with aspirin, the analgesic effects of GHZXT on mice were investigated with hot plate and torsive body method. RESULTS: The clotting time of mice was remarkably shortened with a rising of the dosage of GHZXT and the difference between each therapeutic group and distilled water group was remarkable. As compared with distilled water group, the bleeding time of each dosage group of GHZXT was obviously shortened; and each dosage of GHZXT could prolong the time of pain reaction to hot plate and decrease the degree of torsive body of the mice. CONCLUSION: Pharmacological experiment has proved that GHZXT has evident hemostatic and analgesic function.

Studies in the protective effect of Shenmai injection(参麦注射液) on peritoneal mesothelial cell in rats with 5/6 nephrectomy / 中国中西医结合急救杂志

Objective:To study the protective effects of Shenmai injection (SMI,参麦注射液) on peritoneal mesothelial cell in rats with 5/6 nephrectomy. Methods:By using light microscopy and scanning electron microscopy the morpholgical structure changes of peritoneal mesothelial cell in rats with 5/6 nephrectomy caused (chronic)renal failure model of after the peritoneal didysis of SMI were observed at the same time the levels of C3 and IgG in peritoneal dialysate were observed also. Results:The SMI could promote the recovery and proliferation of injured peritoneal mesothelial cell increase the immunity function of abdomen in rats. Conclusions:The SMI has protective effect on peritoneal cell in rats with 5/6 nepherctomg the protecive effect related to improving regional defence function in abdomen.