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Objective:To explore the therapeutic effect of bicalutamide on prostate cancer.Methods:Ninety-five patients with prostate cancer in the First People's Hospital of Jiande were selected from January 2014 to January 2019.All 95 patients received blood samples before treatment were selected as case group, and 85 healthy subjects were selected as control group.The clinical symptoms of the patients before treatment and after treatment with bicalutamide for 3, 6, 9, 12 months were recorded.The blood samples were taken as the treatment group at 12 months after treatment.The effect of bicalutamide on the expression of miRNA-125b and miRNA-21 in serum of patients with prostate cancer was detected by RT-PCR.Results:After treatment, the prostate volume and serum prostate specific antigen (PSA) expression were significantly reduced(3 months after treatment compared with before treatment, prostate volume: t=6.22, P=0.00; PSA: t=5.14, P=0.00; 6 months after treatment compared with before treatment, prostate volume: t=8.32, P=0.00; PSA: t=4.11, P=0.00; 12 months after treatment compared with before treatment, prostate volume: t=10.14, P=0.00; PSA: t=9.25, P=0.00; 6 months after treatment compared with 3 months after treatment, prostate volume: t=3.82, P=0.00; PSA: t=6.23, P=0.00; effective rate: χ 2=6.38, P=0.00.One year after treatment compared with 3 months after treatment, prostate volume: t=4.16, P=0.00; PSA: t=5.77, P=0.00; effective rate: χ 2=5.74, P=0.00.One year after treatment compared with 6 months after treatment, the prostate volume: t=8.11, P=0.00; PSA: t=4.92, P=0.00; effective rate: χ 2=4.44, P=0.00). And with the extension of treatment time, the treatment effect was significantly enhanced.RT-PCR results showed that compared with the control group, the expression levels of miRNA-125b and miRNA-21 in the case group were significantly increased( t=6.22, 6.88, P=0.01, 0.00). After treatment with bicalutamide, the levels of miRNA-125b and miRNA-21 in serum of prostate cancer patients were significantly reduced. Conclusion:Bicalutamide can reduce the pain and increase the efficacy of prostate cancer patients; bicalutamide can reduce the expression of miRNA-125b and miRNA-21 in serum of patients with prostate cancer.
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Objective To investigate the clinical value of antibody D5F3 and Survivin in patients with non small cell lung cancer.Methods 200 paraffin embedded specimens of patients with non small cell lung cancer tested by RT-PCR(including EML4-ALK mutant and wild type)from October 2012 to June 2014 were selected.The gene protein expression were tested by ALK new antibody D5F3 and the sensitivity and specificity were compared by negative and positive(+～3+).ResultsTest results show(+)the coincidence rate is 15.78％,(+ +)the coincidence rate is 27.27％and(+ + +)compliance rate was 87.5％,the difference was statistically significant(P<0.05).The expression of Survivin protein in NSCLC tissues was correlated with clinicopathological features.The positive rate of Survivin protein expression was correlated with the degree of differentiation(P<0.05),but not with other clinical and pathological features,with the decrease of differentiation,the positive rate of Survivin protein expression was significantly increased.Conclusion D5F3 and Survivin antibodies are highly sensitive and specific in patients with NSCLC,with the screening value,save social resources,for the majority of patients with lung cancer services.
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Objective To identify the configuration of the fascial sheath of the sciatic nerve in the adult pelvis . Methods A total of 14 cadaveric pelvic specimens and 20 living subjects were used for cadaveric examination and magnetic resonance imaging ( MRI) observation , respectively .The cadaveric specimens were investigated by the local latex injection method ( n =10 ) sectional anatomy ( n=2 ) or histological staining methods ( n =2 ) .The MR images were collected and analyzed from twenty patients without pelvic disorders .Results The injected latex did not diffuse along the pelvic segment of the sciatic nerve .Sectional anatomical and histological data showed that the architecture of the fascial sheath of the sciatic nerve varied at different levels .An intact circular sheath around the nerve was rarely observed .There was no fascia barrier between the obturator and sciatic nerves .Appearance on MRI was consistent with the cadaveric observation.Conclusion No intact fascial sheath exists along the pelvic segment of the sciatic nerve , thus sciatic anesthesia may block the obturator nerve .
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220 cases of tympani membrane perforated were summarized retrospectively. The tympani membrane perforated of injury was different from otitis media's because they had marked or extreme marked difference in sex, age, ear distinction, shape, position, size, degree of hearing damaged, time of healing, cause of perforating and adhesive substance. This difference was relative to mechanism of tympani membrane perforated. The distinction gist of two sorts of tympani membrane perforated was expounded through comparison and analysis. It would be of great value to clinical forensic medical examination.
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220 cases of tympani membrane perforated were summarized retrospectively. The tympani membrane perforated of injury was different from otitis media's because they had marked or extreme marked difference in sex, age, ear distinction, shape, position, size, degree of hearing damaged, time of healing, cause of perforating and adhesive substance. This difference was relative to mechanism of tympani membrane perforated. The distinction gist of two sorts of tympani membrane perforated was expounded through comparison and analysis. It would be of great value to clinical forensic medical examination.