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Objective@#To prepare a composite membrane by chitosan/β-sodium glycerophosphate(CS/β-GP) thermosensitive hydrogel combined with stromal cell derived factor-1(SDF-1) and observe its biological characteristics in vitro.@*Methods@#Different doses of SDF-1 were added into CS/β-GP solution and then the thermosensitive gel time was measured. The SDF-1/CS/β-GP solution was membrane paved and dried to prepare composite membranes. The morphological characteristics were observed by scanning electron microscope(SEM). Composite membranes were placed into cell culture medium, and the supernatant(n=3) was extracted after standing at 6, 12, 24, 36, 48, 60 h, respectively. The concentration of SDF-1 in the solution was measured. Bone mesenchymal stem cells(BMSCs) were cultured in the Transwell room, and the composite membranes containing different concentrations of SDF-1 were placed in the lower chamber. There were four groups(n=3): Group M0 used CS/β-GP membrane(control group), Group M1, M2, M3 used SDF-1/CS/β-GP membrane(SDF-1 was 100, 200, 400 ng/mL respectively). After culture for 6, 12 and 24 h, the cells under the membrane were preserved and Giemsa stained and counted. The absorbance(OD) value was measured by MTT method to calculate the cell proliferation rate. SPSS 19.0 was used for multi-factor analysis of variance.@*Results @#After adding a certain amount of SDF-1 into CS/β-GP solution, the gel time did not change significantly(P>0.05). The SDF-1/CS/β-GP membrane was translucent and porous at 37 ℃. In this experiment, the volumic mass of SDF-1 released by SDF-1/CS/β-GP composite membrane increased gradually with the experimental time(P<0.01). Transwell cell chemotaxis test showed that the number of BMSCs cells with directional migration increased with the prolongation of observation time(P<0.01) and the increase of SDF-1 volumic mass(P<0.01). In MTT test, the OD value of migration cell solution increased with the prolongation of time(P<0.01) and the increase of SDF-1 volumic mass(P<0.01). @*Conclusion@# The SDF-1/CS/β-GP composite membrane has a porous structure and biological activity of chemotactic BMSCs directional migration. It is a potential membrane for guided tissue regeneration.
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To explore the clinical efficacy and possible mechanism of Tai Ji Quan for post-stroke depression (PSD), literature related to Tai Ji Quan and PSD were retrieved from China National Knowledge Infrastructure (CNKI), Wanfang Academic Journal Full- text Database (Wanfang), Chongqing VIP Database (CQVIP), China Biology Medicine Disc (CBM), and PubMed, screened and then summarized. The results showed that Tai Ji Quan could effectively improve the depression and quality of life of stroke patients, and there were differences in the clinical efficacy among different training programs. Tai Ji Quan has the characteristics of "regulating mind", "regulating breath", and "regulating body". It may achieve the effect of "combined physique-spirit treatment" by improving social psychology, increasing the level of neurotrophin, regulating neuroendocrine, reducing inflammatory factors, and regulating neural circuits.
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<p><b>OBJECTIVE</b>To investigate the influence of different crown cutting mode on operation time and post-operation trauma in extracting the mandible third molar by dental drilling system.</p><p><b>METHODS</b>According to different impaction types, the patients were divided into vertical impaction, horizontal impaction, and mesioangular impaction groups. The operation time, mouth opening, swelling, and pain degrees were recorded during traditional extraction, crown "T"-shaped cutting, crown three section cutting, and crown-root cutting in the extraction of mandibular wisdom teeth.</p><p><b>RESULTS</b>In the vertical impaction group, the operation time, the degree of swelling, limitation of mouth opening and the pain degree were (8.2 ± 2.5) min, (14.7 ± 2.0) mm, (3.9 ± 2.4) mm and 3.4 ± 1.8, respectively using crown cutting, and (14.0 ± 2.7) min, (18.2 ± 1.9) mm, (9. 7 ± 3.6) mm and 6.9 ± 2.3 using traditional extraction method (P < 0.05). In the horizontal impaction group, the operation time, degree of swelling, limitation of mouth opening and pain degree (14.0 ± 2.0) min, (19.2 ± 3.9) mm, (9.5 ± 2.5) mm, 5.6 ± 1.7 respectively using crown "T"-shaped cutting, and (18.0 ± 3.1) min, (23.6 ± 3.5) mm, (9.8 ± 2.7) mm and 8.5 ± 2.3 using crown three section cutting. There was significant difference in operation time, degree of swelling and pain (P < 0.05), but no significant difference in the limitation of mouth opening (P > 0.05). In mesioangular impaction group, operation time, degree of swelling, limitation of mouth opening and pain degree were (10.2 ± 2.6) min, (18.4 ± 2.9) mm, (6.4 ± 2.5) mm and 4.8 ± 1.8, respectively using "T"-shaped cutting, and (15.0 ± 3.5) min, (18.9 ± 3.0) mm, (7.0 ± 3.7) mm and 7.6 ± 2.3 using crown- root cutting. The operation time and pain degree were significantly different (P < 0.05), but not different in limitation of mouth opening and swelling degree (P > 0.05).</p><p><b>CONCLUSIONS</b>Different impaction types of wisdom teeth should choose different cutting mode. Vertical impacted tooth uses distal crown cutting. "T"-shaped cutting is preferred with horizontal impaction. According to the angle and depth of the mesioangular impaction, "T"-shaped cutting or the crown-root cutting can be selectively used.</p>
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Humanos , Mandíbula , Dente Molar , Dente Serotino , Coroa do Dente , Extração Dentária , Métodos , Raiz Dentária , Dente ImpactadoRESUMO
Objective To analyze the initial response rate of radiotherapy and chemotherapy,and the influence of different treatment modalities on pmgnnsis of primary non-Hodgkin lymphoma(NHL)of the nascl cavity.Methods From January 1993 to December 2002,70 patients were confirmed by histopathology as NHL,including 52 T cell origin,2 B cell and 16 NK cell.The main radiotherapy portal was set in front of the nose with a spade like protrusion,supplement with a portal next to the ear on one side or both sides.Combined portal in facial cervical area was first used when there was invasion of the oropharynx.The median dose to the nasal cavity wag 54.0 Gy with a range of 336 to 66 Gy,given before or during after radiotherapy or alone,consisted of 2-6 cycles chemotherapy of COP,CHOP or COBDP.Prognostic factors were analyzed with Cox model. Results The local control rates was 12.5%, 66.7% and 74.0% in chemotherapy alone,radiotherapy alone,and chemotherapy plus radiotherapy.The 5-year survival rate Wag 12.5%,50.0% and 62.0%,respectively(P＜0.05).Multifaetor analysis showed that tumor extension and treatment methods were the most important prognostic factors besides Ann-Arbor stage,but gender,pathology,age and symptoms had little effect on prognosis.Conclusion Chemotherapy plus radiotherapy group achieved a better survival rate than radiotherapy alone.For some NHL patients with good financial condition,APBSCT is a good choice.