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Background@#Acne vulgaris (AV) is a common adolescent skin condition which is mainly caused by Cutibacterium acnes overcolonization and subsequent inflammation. @*Objective@#Our previous studies demonstrated that ethanol extracts of Meconopsis quintuplinervia Regel (EMQ) possess significant antimicrobial properties. However, their protective effects and potential mechanisms against AV remain unclear. @*Methods@#In the present study, the EMQ treatment potential for AV was evaluated in a C.acnes-induced mouse ear edema model, and the EMQ anti-inflammatory mechanism was evaluated in lipopolysaccharide (LPS)-induced RAW264.7 macrophage cells. @*Results@#The results showed that EMQ alleviated edema formation and inflammatory cell infiltration in an acne mouse model by suppressing inflammatory cytokines interleukin (IL)-6, IL-1β, and tumor necrosis factor α expression. Moreover, EMQ inhibited the phosphorylation of MAP kinases (MAPKs) such as p38, JNK, and ERK, the phosphorylation and degradation of IκB-α and the nuclear translocation of nuclear factor kappa B (NF-κB) p65 in LPS-induced RAW264.7 cells. @*Conclusion@#These findings suggest the potent anti-inflammatory activity of EMQ is possibly through the regulation of the MAPKs and NF-κB signaling pathways. Inhibition of C. acnes activity combined with a powerful anti-inflammatory effect of EMQ indicated its potential as a novel therapeutic option for AV.
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In 2006, 2014 and 2020, the positive rates of HBsAg in 560, 384 and 402 children aged 1 to 14 years were 4.5%, 2.6% and 2.5%, respectively, with no statistically significant differences (P>0.05). The positive rate of anti-HBs was highest in 2014 (57.8%) and lowest in 2006 (34.1%) (P<0.05). The positive rate of anti-HBc was highest in 2006 (15.7%), and decreased in 2014 (7.8%) and 2020 (5.7%) (P<0.001). The timely rate of the first dose of hepatitis B vaccine for children in Lhasa in 2006, 2014 and 2020 was 7.7% (43/560), 50.3% (193/384) and 94.8% (381/402), respectively. The overall vaccination rates were 15.4% (86/560), 35.2% (135/384) and 96.0% (386/402), respectively, showing a trend of gradual increases (χtrend values were 718.63 and 589.59, both P values<0.001).
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Criança , Humanos , Vacinas contra Hepatite B , Hepatite B/prevenção & controle , Antígenos de Superfície da Hepatite B , Vírus da Hepatite B , Anticorpos Anti-Hepatite B , VacinaçãoRESUMO
OBJECTIVE:To study the effec ts of Tibetan Codonopsis tralictrifolia extract (called“ZDS”for short ) on collagen-induced arthritis (CIA)model rats and its mechanism. METHODS :Eight of 48 rats were randomly selected as normal control group (normal saline ),and the remaining 40 rats were used to establish CIA model. After successful modeling ,the rats were randomly divided into model group (normal saline ),ZDS low-dose ,medium-dose and high-dose groups (0.44,0.88,1.76 g/kg,by crude drug ),dexamethasone group (positive control ,0.002 5 g/kg),with 8 rats in each group. They were given relevant medicine intragastrically ,the volume of 400 μL,once a day ,for consecutive 28 days. The body weight of rats were weighed before medication (0 d),7,14,21 and 28 days after medication ;and arthritis indexes were scored. The pathological changes of the knee joint synoviual tissue were observed after last medication. The thymus index ,spleen index ,the levels of serum inflammatory factors (IL-1β,TNF-α,IL-6),protein expressions of NF-κB p65,p-NF-κB p65,IκB and p-IκB in synovial tissue were detected. RESULTS :Compared with normal control group ,the body weight (14,21,28 days after administration )of rats in model group was significantly reduced (P<0.05);the arthritis index score (before administration and different administration time)was significantly increased (P<0.05);the joint synovial tissue was pathologically damaged ;the thymus and spleen index , inflammation factor level ,the protein expression of p-NF-κB p65 and p-IκB were increased significantly(P<0.05),while the protein expression of IκB was decreased significantly(P<0.05). Compared with model group ,the level of IL- 1β was decreased significantly in ZDS low-dose group (P<0.05). Body weight of rats (21,28 days after administration )were increased significantly in ZDS medium-dose and high-dose groups ,dexamethasone group (P<0.05),while arthritis index score (14,21,28 days after administration ) was decreased significantly (P<0.05). The pathological damage of joint synovial tissue was significantly relieved ;thymus and spleen index ,inflammation factor level ,the protein expression of p-NF-κB p65 and p-IκB were significantly reduced,while the protein expression of IκB was significantly increased (P<0.05). CONCLUSIONS :ZDS can improve CIA model rats to some extent ,and its mechanism may be related to the inhibition of NF-κB signaling pathway.
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Objective:To clone cellulose synthase-like(Csl)gene from ethnic medicinal plant Ampelopsis megalophylla,and analyze its sequence by bioinformatics. Method:Specific primers were designed for AmCsl gene sequences obtained from A. megalophylla transcriptome sequencing data. The full-length cDNA of AmCsl gene was amplified by PCR using cDNA of young leaves as template,and TA clone and sequencing was performed. The sequence was analyzed by bioinformatics. Result:The full length cDNA was 1 438 bp,containing a 561 bp open reading frame(ORF),and encoding 186 amino acids,the molecular formula of protein was C1011H1547N233O257S10,the theoretical relative molecular weight was 22.40 kDa,the theory isoelectric point(PI)was 7.59,and the aliphatic index(AI)was 116.88.There was a transmembrane region and no signal peptide,which may be located in the endoplasmic reticulum,the average hydrophobic coefficient was 0.670,and the instability index was 42.56.It belonged to a hydrophobic unstable protein. The conserved domain contained a cellulose synthase,and the secondary structure mainly was dominated by α-helix. Amino acid sequence alignment and phylogenetic tree analysis showed that AmCsl had a high homology with Vitis vinifera. Conclusion:The full length of AmCsl gene was obtained for preliminary bioinformatics analysis,which laid a necessary foundation for further study on the accumulation of polysaccharides and the regulation of biosynthesis.
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Pediatric epilepsy is one of the most common neurological disorders in childhood,and it has a huge impact on the patients′life and their families.There has been rapid development in the knowledge of epilepsy genetics.Several genetic tests are now available for clinical practice.This is important for the diagnosis, treatment, prognosis and genetic counseling.The modern genomic technologies for the diagnosis of patients with genetic epilepsies are reviewed.The potential challenges of the interpretation of the genetic reports are also discussed.
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Objective@#To investigate the correlation of intermittent androgen-deprivation therapy (IADT) and continuous androgen-deprivation therapy (CADT) for advanced prostate cancer (PCa) with the risks of secondary diabetes mellitus (DM) and impaired glucose tolerance (IGT).@*METHODS@#We conducted a retrospective case-control study of the advanced PCa patients treated by IADT or CADT in our hospital from January 2013 to December 2015. Based on the levels fasting blood glucose and 2-hour postprandial blood glucose, results of oral glucose tolerance test, and clinical symptoms of the patients, we statistically analyzed the IADT- or CADT-related risk factors for DM and IGT and the relationship of the body mass index (BMI), hypertension, smoking, and alcohol consumption with secondary DM and IGT.@*RESULTS@#IADT was given to 53 (46.5%) of the patients, aged (69.1 ± 4.3) years, and CADT to 61 (53.5%), aged (70.2 ± 5.7) years. No statistically significant differences were observed in clinical characteristics between the two groups of patients (P > 0.05). BMI, blood pressure, smoking and drinking exhibited no significant influence on the development of DM or IGT either in the IADT (P > 0.05) or the CADT group. The incidence of IGT was significantly lower in the IADT than in the CADT group (P = 0.03), but that of DM showed no statistically significant difference between the two groups (P = 0.64).@*CONCLUSIONS@#Compared with CADT, IADT has a lower risk of IGT and a higher safety in the treatment of advanced prostate cancer.
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Idoso , Humanos , Masculino , Consumo de Bebidas Alcoólicas , Antagonistas de Androgênios , Usos Terapêuticos , Glicemia , Metabolismo , Índice de Massa Corporal , Estudos de Casos e Controles , Diabetes Mellitus , Intolerância à Glucose , Teste de Tolerância a Glucose , Hipertensão , Neoplasias da Próstata , Tratamento Farmacológico , Patologia , Estudos Retrospectivos , Fatores de Risco , FumarRESUMO
ENHANCIN is an enhancing protein chiefly found in insect baculoviruses. One ENHANCIN homologue was identified, by blast method, in Agrotis Segetum granulovirus (AgseGV) genome, named enhancin-like. Sequence analysis indicated that this gene includes the conserved domains, conserved in other ENHANCIN, and it has no signal peptide or a-transmembrane helix. A proline-rich domain, which is similar to those of mammals, is present at its C-terminal. To analyze the synergistic function of AgseGV enhancin-like gene, prokaryotic expression vectors of its whole gene and the 5'-truncated fragment (1, 017bp) were constructed. Expression product of truncated fragment was purified by chromatography, and then it was used to prepare antibody. The expression product of whole gene was identified by Western blot with specific antibody and anti-His-Tag antibody. Bioassay proved that the expression product of whole gene can increase the mortality with 16.25% to 3th instar larvae of Helicoverpa armigera (HaNPV: 1.17 x 10(2) PIBS/mL), while the truncated fragment has no obvious synergistic function.
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Animais , Sequência de Aminoácidos , Baculoviridae , Genética , Metabolismo , Expressão Gênica , Controle de Insetos , Larva , Dados de Sequência Molecular , Mariposas , Controle Biológico de Vetores , Proteínas Virais , Genética , Metabolismo , ToxicidadeRESUMO
Objective To investigate the distribution and expression changes of voltage-gated chloride channel CLC-2 and CLC-3 in rat models with lithium-pilocarpine-induced chronic epileptic,and discuss the significance of these changes in epileptic pathogenesis.Methods Eighty Wistar rats were randomly divided into status epilepticus(SE,n=60)and control(n=20)groups and rats in the SE group were assigned to 3 subgroups according to the different sacrificed times(24 h,14 d and 30 d).Rat models with chronic epileptic in the SE group were induced by lithium-pilocarpine.Immunohistochemistry was employed to observe the changes of voltage-gated chloride channel CLC-2 and CLC-3 in rat's hlppocampal formation different time points after seizure.The mRNA changes of chloride channel CLC-2 and CLC-3 at different time points after seizure were observed by RT-PCR.Results Compared with those in the control group,the quantity of CLC-2 immune positive neurons and the average optical density in the SE group decreased obviously 14 and 30 d after seizure;so was the mRNA expression of CLC-2(P<0.05).Compared with the control group,the SE group showed obviously increased quantity of CLC-3 immune positive neurons and optical density at each area of the hippocampus 24 h after seizure;so was the mRNA expression of CLC-3(P<0.05).Conclusion Seizures at chronic phase have relation with the decreased expressions of CLC-2.