Endothelial progenitor cells with overexpressing angiotensin-converting enzyme 2 reduce monocrotaline-induced pulmonary arterial hypertension in rats / 基础医学与临床

Objective To investigate the therapeutic effect of endothelial progenitor cells (EPCs) with overex-pressed angiotensin converting enzyme 2(ACE2) on rat model of monocrotaline(MCT) induced pulmonary arterial hypertension (PAH). Methods EPCs were extracted,purified and identified from the very beginning. The lenti-virus vector carrying ACE2 gene was infected with EPCs in order to prepare the ACE2-EPCs. Besides,the expres-sion of ACE2 and proliferation as well as tube function of EPCs were detected before and after the transduction. The SD rat were randomly divided into control group, MCT induced pulmonary arterial hypertension group (PAH group),ACE2 transduction of endothelial progenitor cells group(lenti-ACE2-EPCs group) and endothelial progenitor cells group (EPCs group).The mean pulmonary artery pressure of rats(mPAP) and right ventricular hypertrophy in-dex (RV/LV+S) were determined in third week after the molding;the lung tissue specimens were stained with HE and the pulmonary arterial wall thickness index(TI) as well as area index(AI) were also calculated;The expression levels of ACE2 in lung tissue were detected by Western blot.Results The mPAP,RV/LV+S,TI and AI detected in PAH group in the third week after the molding were signficantly lower than those in control group(P<0.01); The mPAP,RV/LV+S,TI and AI detected in lenti-ACE2-EPCs group in the third week after the molding were markedly lower than those in PAH group and EPCs group (P<0.01); The expression level of ACE2 in lung tissue of lenti-ACE2-EPCs group was significantly higher than that in PAH group (P<0.01);The mPAP,RV/LV+S,TI and AI detected in EPCs group in the third week after the molding were markedly lower than those in PAH group(P<0.01), while higher than that as compared with control group and lenti-ACE2-EPCs group (P<0.01). Conclusions ACE2 overexpression in endothelial progenitor cells(EPCs) can effectively reduce the mean pulmonary arterial pressure in PAH rat models,and the therapeutic effect of ACE2-EPCs is generally better than that of EPCs.

Effect of liposuction on adipokines, inflammatory markers and insulin resistance / 中华整形外科杂志

<p><b>OBJECTIVE</b>To study the effect of large-volume liposuction on adipokines, inflammatory markers and insulin resistance and its significance.</p><p><b>METHODS</b>The levels of serum adiponectin, resistin, IL-6, TNF-alpha, CRP and insulin sensitivity were measured pre- and 3 months postoperatively in 16 consecutive patients undergoing liposuction.</p><p><b>RESULTS</b>The adiponectin postoperative [(5.56 +/- 1.03) ng/ml] was significantly higher than the preoperative [(6.54 +/- 1.27) ng/ml] (P<0.05); the resistin postoperative [(6.30 +/- 3.25) pg/ml] is significantly lower than the preoperative [(5.18 +/- 2.28) pg/ml] (P<0.05); the insulin sensitivity postoperative (-3.78 +/- 0.39) significantly increased compared to the preoperative (-3.48 +/- 0.32, P<0.05); the TNF-alpha postoperative [(0.19 +/- 0.02) pg/ml] significantly increased compared to the preoperative [(0.23 +/- 0.03) pg/ml, P<0.01]; the postoperative IL-6 [(0.27 +/- 0.04) pg/ml] was similar to the preoperative [(0.31 +/- 0.06) pg/ml, P >0.05]; the postoperative CRP [(8.03 +/- 6.01) mg/L] was not significantly different with the preoperative [(7.28 +/- 6.21) mg/L, P>0.05].</p><p><b>CONCLUSIONS</b>Large-volume liposuction has good effect on insulin resistance and adipokines.</p>

Trilobate technique, a new principal to repair cleft lip / 中华整形外科杂志

<p><b>OBJECTIVE</b>To develop a new method for reparation of cleft lip, and to evoke more colleagues for advance practices and study, in order to determine her indication and contraindication as soon as possible.</p><p><b>METHODS</b>48 cases were included into this study. Trilobate flap were designed in floor of nose and lip area in cleft side, rotate two of the three flaps upwards, respectively to elevate the tip of nose, and to reconstruct the floor of nose. As for the left flap, it was derived transversally to opposing side, sutured with the flap of non-cleft-side.</p><p><b>RESULTS</b>With this technique, less tissue was lost, better vertical lengthening and good formed cuspids-bow was achieved, and the scar was a parallel line being symmetry to the philtrum column opposite. Meanwhile, because the tension was mainly located in the area where there was no mini flaps, the blood supply was good enough, rarely occur any necrosis in the tip of flaps. All cases in this study obtained perfect healing, with good appearance at nostrils and floor of nose.</p><p><b>CONCLUSIONS</b>In use of the method of trilobate flap, we can draw down the peak of the cuspids bow effectually, hence avoid the addition cut in the lower part of the lip, decrease the scar on skin, as well as nice reconstruction of floor of nose, philtrum column and nostril. Because lack of long term study, we evoke more colleagues for cooperation in advance study.</p>

Arachidonic acid epoxygenases and their metabolites promote angiogenesis / 中华心血管病杂志

<p><b>OBJECTIVE</b>To investigate the angiogenetic effects of endogenous and exogenous epoxyeicosatrienoic acids (EET) and the relevant signaling mechanisms involved.</p><p><b>METHODS</b>Bovine aortic endothelial cells (BAEC) were incubated with synthetic EETs or infected with recombinant adeno-associated viruses (rAAV) containing CYP2C11-CYPOR, CYP2J2 or CYP102 F87V mutant to increase endogenous expression levels of EETs. BAEC proliferation measured by cell counting and chromatometry, migration assessed by transwell analysis, and capillary formation determined by chicken embryo chorioallantoic membrane assays (CAM) and tube formation tests on matrigel and angiogenesis were analysed in vivo. The potential involvement of various signaling pathways were explored using selective inhibitors.</p><p><b>RESULTS</b>Transfection with rAAV-2C11OR, rAAV-2J2 or rAAV-F87V promoted BAEC proliferation, migration, and capillary tubule formation. However, the effects of EETs on proliferation, migration and capillary tubule formation were attenuated by inhibitors of mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3 kinase)/Akt pathways, and partially attenuated by endothelial nitric oxide synthase (eNOS) inhibitor, but not by a protein kinase C inhibitor. In a rat ischemic hind limb model, rAAV-mediated epoxygenase transfection induced angiogenesis.</p><p><b>CONCLUSIONS</b>Arachidonic acid epoxygenase and its metabolites can promote angiogenesis through activating MAPK and PI3 kinase/Akt signaling pathways, and to some extent, the eNOS pathway, and the angiogenic effects may provide protection to ischemic tissues.</p>

Study on the distribution of serum homocysteine and on multi-stepwise regression analysis of the associated factors in the population of community areas in Wuhan / 中华流行病学杂志

<p><b>OBJECTIVE</b>To study the serum homocysteine (Hcy) distribution and characteristics in different sex and age groups in the community residents in Wuhan, and to analyse its associated factors with multi-stepwise regression analysis.</p><p><b>METHODS</b>The population under study was from three community areas in Wuhan. Demographic distribution and the correlation with other risk factors of serum Hcy were analyzed statistically.</p><p><b>RESULTS</b>(1) Geometric mean of serum Hcy was 14.43 micromol/L in males and 10.89 micromol/L in females with P <0.001. (2) Hcy of per age level in males was also higher (P <0.001). (3) The prevalence rate of hyperhomocysteinemia was 23.94% in the general population in Wuhan. The prevalence rate of hyperhomocysteinemia in males was 2.62 times higher than in females. (4) Multi-stepwise regression analysis showed that Hcy had different affecting factors in males and females. The affecting factors of Hcy in males were daily cigarettes smoking, urine micro-albumin (UMALB) and times of exercise per week. The affecting factors of Hcy in females were duration of exercise each time, weight, triglyceride (TG), high-density lipoprotein (HDL), urine micro-albumin (UMALB) and age.</p><p><b>CONCLUSIONS</b>(1) Hcy at the population level was significantly different by sex and age. (2) Population living in the community in Wuhan had a higher serum level and prevalence rate of Hcy comparing to some other cities in China and even in developed countries. (3) The important affecting factors of Hcy in population also showed sex difference, unlike the reports from other countries or other areas in China. Serum Hcy seemed to be affected by environmental and other factors.</p>