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1.
Journal of Southern Medical University ; (12): 262-265, 2011.
Artigo em Chinês | WPRIM | ID: wpr-307955

RESUMO

<p><b>OBJECTIVE</b>To investigate the biological features of human decidua basalis-derived mesenchymal stem cells (PDB-MSCs) in vitro and identify their capacity of multilineage differentiation.</p><p><b>METHODS</b>PDB-MSCs were harvested from the decidua basalis of term placental by enzymatic digestion and density gradient centrifugation, and the growth characteristics and morphological changes of the MSCs were observed by inverted microscope. The proliferative ability of the cells was assessed by Cell Counting Kit-8. The cell cycle and expressions of the surface markers (CD29, CD44, CD73, CD90, CD34, CD45, and CD14) of the MSCs were identified by flow cytometry. Multilineage differentiation capacity of the cells was tested by inducing their differentiation toward osteoblasts, adipocytes and chondroblasts in vitro.</p><p><b>RESULTS</b>MSCs isolated from human decidua basalis of term placental exhibited a morphology similar to that of bone marrow-derived MSCs, and grew into colonies in in vitro culture, where the cells proliferated rapidly after passage with a cell doubling time of 2.21∓0.21 days. More than 70% of the cells stayed in the resting stage (G(0)/G(1)) and showed positivity for CD29, CD44, CD73 and CD90, but not for CD14, CD34 or CD45. After induction, the cells showed positive results of alizarin red staining, oil red O staining and Alcian blue staining.</p><p><b>CONCLUSION</b>Human decidua basalis contains a rich source of MSCs, which can be easily isolated and cultured without affecting their capacity of multilineage differentiation. The PDB-MSCs may have the potential as a novel source of stem cells.</p>


Assuntos
Feminino , Humanos , Gravidez , Diferenciação Celular , Fisiologia , Separação Celular , Células Cultivadas , Decídua , Biologia Celular , Células-Tronco Mesenquimais , Biologia Celular , Células-Tronco Multipotentes , Biologia Celular , Placenta , Biologia Celular
2.
Journal of Southern Medical University ; (12): 439-442, 2010.
Artigo em Chinês | WPRIM | ID: wpr-355106

RESUMO

<p><b>OBJECTIVE</b>To construct the eukaryotic expression vector pDsRed2-N1-SDF-1alpha and observe its expression in the mouse bone marrow mesenchymal stem cells.</p><p><b>METHOD</b>SDF-1alpha gene sequence with XhoI, EcoRI restriction enzyme cutting site was amplified from the total RNA of mouse smooth muscle cells by reverse transcription-polymerase chain reaction (RT-PCR) and inserted into the eukaryotic expression vector pDsRed2-N1 encoding red fluorescent protein gene, and the insertion was verified by endonuclease digestion and DNA sequencing. Mouse bone marrow mesenchymal stem cells identified with immunofluorescence assay for vimentin expression were transfected with the constructed plasmid pDsRed2-N1-SDF-1alpha, and the expression of sdf-1alpha was detected using immunofluorescence assay.</p><p><b>RESULTS</b>The DNA fragment amplified by PCR from the total RNA was identical to SDF-1alpha from the gene library, and an identical DNA fragment was also amplified from the recombinants. Sequence analysis confirmed the successful insertion of SDF-1alpha into the pDsRed2-N1 vector and the eukaryotic expression vector pDsRed2-N1-SDF-1alpha was successfully constructed. The cultured mouse bone marrow mesenchymal stem cells positive for vimentin protein showed SDF-1alpha expression 24 h after transfection with the recombinant vector.</p><p><b>CONCLUSION</b>The pDsRed2-N1-SDF-1alpha eukaryotic expression vector constructed is capable of expression of SDF-1alpha fusion protein in the mouse bone marrow mesenchymal stem cells.</p>


Assuntos
Animais , Feminino , Camundongos , Células da Medula Óssea , Biologia Celular , Metabolismo , Quimiocina CXCL12 , Genética , Vetores Genéticos , Células-Tronco Mesenquimais , Metabolismo , Camundongos Endogâmicos C57BL , Proteínas Recombinantes de Fusão , Genética , Transfecção
3.
Chinese Journal of Medical Instrumentation ; (6): 182-183, 2005.
Artigo em Chinês | WPRIM | ID: wpr-344219

RESUMO

An intelligent control system has been designed using the single chip and the related circuit, and with the assemble language. It is connected with the common X-ray units to control the exposure dose. The result shows that three parameters for radiography are well controlled by the intelligent control system, and auto-radiography is realized.


Assuntos
Algoritmos , Inteligência Artificial , Simulação por Computador , Sistemas Computacionais , Processamento de Imagem Assistida por Computador , Armazenamento e Recuperação da Informação , Métodos , Radiografia , Métodos , Design de Software , Interface Usuário-Computador
4.
Chinese Journal of Experimental and Clinical Virology ; (6): 176-178, 2005.
Artigo em Chinês | WPRIM | ID: wpr-333069

RESUMO

<p><b>OBJECTIVE</b>To establish a simple rapid and sensitive nested RT-PCR method for detection of SARS coronavirus RNA by designing the specific primers for SARS and optimizing the parameters for PCR.</p><p><b>METHODS</b>Primers and fluorescent probes were designed according to the sequences of SARS coronavirus genes available from GenBank. The optimization of the parameters for PCR was performed in PE 7700 thermal cycle. The 36 serum samples and 40 mouthwash of SARS patients and 80 samples of healthy people were tested.</p><p><b>RESULTS</b>The positive rate of patient serum and mouthwash was 33.6%, (12/36) and 67.5%, (27/40), respectively, while the positive rate of healthy people was zero (0/160).</p><p><b>CONCLUSION</b>The simple nested RT-PCR method was a rapid, efficient and sensitive one for SARS early diagnosis.</p>


Assuntos
Humanos , Secreções Corporais , Virologia , Primers do DNA , RNA Viral , Sangue , Genética , Reprodutibilidade dos Testes , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Métodos , Coronavírus Relacionado à Síndrome Respiratória Aguda Grave , Genética , Sensibilidade e Especificidade , Síndrome Respiratória Aguda Grave , Sangue , Diagnóstico , Virologia
5.
Chinese Journal of Experimental and Clinical Virology ; (6): 291-293, 2004.
Artigo em Chinês | WPRIM | ID: wpr-279550

RESUMO

<p><b>OBJECTIVE</b>To develop a method for detection of coxsackie B virus type 1-6 by RT-PCR.</p><p><b>METHODS</b>A pair of primers were designed to amplify all types of coxsackie B virus 1-6 efficiently. The PCR product was hybridized in micro-wells in which 6 type specific oligonucleotide probes had been coated respectively, colorimetric detection was performed to discriminate the types of coxsackie B virus.</p><p><b>RESULTS</b>This method was shown to be concordant with the IgM ELISA, 71.7% of anti-coxsackie B positive cases could be detected by RT-PCR.</p><p><b>CONCLUSION</b>The RT-PCR method can type coxsackie B virus efficiently and provides a tool for clinical diagnosis and epidemiological investigation.</p>


Assuntos
Humanos , Primers do DNA , Enterovirus Humano B , Classificação , Genética , Infecções por Enterovirus , Diagnóstico , Virologia , Ensaio de Imunoadsorção Enzimática , Imunoglobulina M , Sangue , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Métodos
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