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1.
Korean Journal of Veterinary Research ; : 43-48, 2013.
Artigo em Coreano | WPRIM | ID: wpr-147389

RESUMO

Commercial bivalent killed Salmonella vaccine Salenvac-T has been used in several countries in order to prevent salmonellosis with Salmonella enterica serovars Enteritidis (SE) and Typhimurium (ST) in poultry. However, this vaccine has not been used in poultry farms in South Korea. In this study, we evaluated the efficacy of Salenvac-T vaccine to protect against the challenge of virulent SE and ST, and the effect of the vaccine on egg production and mortality in layer hens. The colonization of liver, spleen and cecum with challenged SE and ST was reduced in vaccinated chickens compared with that of unvaccinated control group. The twice vaccination with Salenvac-T induced elevated antibody responses against both SE and ST detected by enzyme-linked immunosorbent assay (ELISA). The higher average hen-day production was observed in the vaccinated layer hens than in the unvaccinated layer hens without significance. The average mortality was lower in the vaccinated layer hens during the experiment period. The antibody responses to both SE and ST were persistently detected in the vaccinated layers. In summary, vaccination with Salenvac-T reduces colonization of internal organs and induces good antibody responses, thereby results in higher performance and lower egg contamination with SE and ST in layer hens.


Assuntos
Formação de Anticorpos , Ceco , Galinhas , Colo , Ensaio de Imunoadsorção Enzimática , Fígado , Óvulo , Aves Domésticas , República da Coreia , Salmonella , Salmonella enterica , Salmonella enteritidis , Infecções por Salmonella , Salmonella typhimurium , Baço , Vacinação
2.
Korean Journal of Veterinary Research ; : 211-216, 2013.
Artigo em Coreano | WPRIM | ID: wpr-200777

RESUMO

This study was focusing on evaluating the protection of polyphosphate kinase (ppk) deleted and/or temperature-sensitive (ts) Salmonella Enteritidis (SE) as an attenuated vaccine in chickens. We constructed SEppk, SEts and SEppk::ts mutants and screened those mutants by growth capability in vitro, protection study in mice model and antibody response in chickens. Among the mutants, SEppk::ts-3 was selected because it showed higher growth capability, good protection against highly virulent SE in mice model, and good antibody response in chickens. SEppk::ts-3 also showed good protection against highly virulent SE isolate because it decreased colonization of virulent SE challenge strain in spleen, liver and cecum compared with the non-vaccinated control. The SEppk::ts-3 mutant showed cross-protection against S. Gallinarum (SG) challenge although the its cross-protection rate was a little lower than that of SG9R, a commercial vaccine against SG infection. To use for live attenuated vaccine in chickens, it should further be characterized.


Assuntos
Animais , Camundongos , Formação de Anticorpos , Ceco , Galinhas , Colo , Proteção Cruzada , Fígado , Fosfotransferases , Salmonella enteritidis , Salmonella , Baço
3.
Korean Journal of Veterinary Research ; : 21-28, 2011.
Artigo em Coreano | WPRIM | ID: wpr-38063

RESUMO

Salmonella Enteritidis (SE) has been a major causative agent of food-borne human disease due to consumption of contaminated eggs and poultry meat. To prevent SE infection in poultry, and therefore minimize human infections, vaccination with either killed or live SE vaccine is suggested. We evaluated a newly developed killed bacterin using a representative SE isolate in Korea. Among pool of SE isolates, two highly virulent isolates (the one isolate from chicken, the other from human) were selected by measuring mortality in mouse and chickens administered. The chickens were injected intramuscularly with killed vaccine and were challenged with highly virulent SE strain 3 week after vaccination. The recovered colony count (cfu/g) of spleen and cecal content in the vaccinated groups was reduced compared with those of the unvaccinated control group. The antibody level in the vaccinated groups was higher at 3 week post vaccination. These results indicate that vaccination with killed vaccine was effective in preventing the infection of virulent SE. Further study for a large number of layers should be needed for the effect of egg production, SE shedding in feces, persistence of antibody level.


Assuntos
Animais , Humanos , Camundongos , Formação de Anticorpos , Vacinas Bacterianas , Galinhas , Ovos , Fezes , Coreia (Geográfico) , Carne , Óvulo , Aves Domésticas , Salmonella , Salmonella enteritidis , Baço , Entorses e Distensões , Vacinação
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