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Objective To study the differentiation potential of mouse spinal cord derived neural stem cells (NSCs) into neurons after being transfected with BDNF gene in vitro. Methods Spinal cord derived NSCs from the E14 fetus mouse were isolated and cultured in vitro; the retrovirus containing pLXSN-BDNF gene was established and transfected into thc above NSCs, and thea, spinal cord derived NSCs were induced to be differentiated into neuron-like cells. Immunohistochemistry was employed to detect and calculate the ratio of differentiation of NSCs into neurons. Results The NSCs cultured in vitro partly adhered to the wall and differentiated within 24 h of transfection with BDNF gene, and most of the NSCs adhered to the wall differentiated within 48 h of transfection. The level of neurons from spinal cord derived NSCs modified by BDNF gene was markedly increased as compared with that that from normal spinal cord derived NSCs (P＜0.05). Conclusion NSCs transfected by retroviral pLXSN-BDNF can promote the cell differentiation. BDNF gene can increase greatly the percentage of neurons in the course of inducing the differentiation of mouse NSCs.
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Objective To investigate the isolation and culture of the neural stem cells (NSCs) derived from the embryonic mouse spinal cord and striatum, and observe the differences in their proliferation and differentiation characteristics in vitro, thereby providing evidences for identifying more suitable seed cells for repainng spinal cord injuries. Methods The spinal cord and striatum of 14-day-old embryonic mice were dissected for primary NSC culture. After 4 passages, the cells were examined for the expression ofnestin, β-tubulin Ⅲ, glial fibrillary acidic protein (GFAP) and MAG using immunohistochemistry. Results The NSCs derived from the spinal cord and the striatum both possessed in vitro proliferation capacity and expressed nestin antigen. After induced differentiation, the NSCs expressed specific antigens of neurons, astrocytes and oligodendrocytes. Conclusion Multipotent NSCs can be obtained from embryonic mouse spinal cord and striatum through different in vitro culture methods under different conditions. According to the differenees between the two kinds of NSCs in culture situation and differentiation, the striatum derived NSC is suitable for transplantation to repair spinal cord injury.
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<p><b>OBJECTIVE</b>To evaluate clinical outcomes of bracing and analyze related factors that influence curative effects in adolescents with idiopathic scoliosis, and to investigate indications of bracing.</p><p><b>METHODS</b>Seventy-nine patients with AIS who had no history of prior therapy were treated with a brace. Several parameters were consecutive measured and documented during the period of follow-up including Cobb's angles, curve patterns, menarche status, sitting heights, standing heights, Risser sign, apical vertebral rotation, and so on.</p><p><b>RESULTS</b>The average period of followed-up was 30 months (12 months to 60 months). Twenty-one patients (26.6%) presented curve deterioration, 40 patients have no obvious curve change, 18 patients (22.8%) got a curve improvement. There was significantly lower percentage of curve progression and higher percentage of curve improvement in cases with Cobb's angle less than 35 degrees at the first visit (P < 0.05). The percentage of curve progression was significantly greater in the cases with apical vertebral rotation beyond grade III while the percentage of curve improvement was lower (P < 0.05). Curve patterns, Risser sign and other parameters were found to make their effects on the percentage of curve progression and improvement, which, however, was not statistically significant (P > 0.05).</p><p><b>CONCLUSION</b>Bracing can limit or improve mild and moderate curve of idiopathic scoliosis effectively, especially in cases with initial curve magnitude ranging from 20 degrees to 35 degrees . Risser sign is not a reliable parameter for measuring the outcome of bracing treatment for idiopathic scoliosis. Surgery is advised as soon as possible for the cases with initial Cobb's angles greater than 45 degrees and initial apical vertebral rotation beyond grade III early while bracing did not work.</p>