RESUMO
Objective To study the histomorphology structure of the femur in adult horses and adults, analyze the histological features and establish the method of identifying the species between humans and horses. Methods The 4 cm mid-diaphyseal segment of the right femur from adult human at autopsy was obtained. At the same time, the right femur of the horse was collected and the middle section was obtained about 4cm. After decalcification, a bone tissue section about 25 μm in thickness was obtained. Observe under an optical microscope, images under a microscope were input into a computer, and 25 indicators were selected for stepwise discriminant analysis. Results Significant differences between horses and human were observed on 13 indicators such as number of Haversian system and Haversian system diameter. Mathematical model for species identification was established based on these indicators. After a blind test,the discrimination reaches 99.6%. Conclusion Horse and human femur histological structure have obvious species characteristics and the established discriminant equation can effectively identify horses and human femur fragments.
RESUMO
Two new sesquiterpenoids, named as tyromols A and B (1 and 2), were isolated from cultures of basidiomycete Tyromyces chioneus, along with two previously reported 15-hydroxy-6 α, 12-epoxy-7β, 10αH, 11βH-spiroax-4-ene (3) and agripilol C (4). Compounds 1-4 were separated and purified by silica gel, RP-18, Sephadex LH-20 column chromatography. Their structures were elucidated on the basis of extensive spectroscopic analysis including IR, MS, 1D and 2D NMR experiments.