Chemical constituents of Uvaria grandiflora / 中国药科大学学报

@#Chemical investigation of the roots of Uvaria grandiflora by repeated column chromatography(CC)over silica gel, ODS, Sephadex LH-20, and HPLC resulted in the isolation of twelve compounds. Their structures were identified as: epicatechin-(4β→1′, 2→O→2′)-phloroglucinol(1), proanthocyanidin A-1(2), proanthocyanidin A-2(3), epicatechin(4), phlorizin(5), eriodictyol(6), erythro-guaiacylglycerol-8-O-4′-(coniferyl alcohol)ether(7), threo-guaiacylglycerol-8-O-4′-(coniferyl alcohol)ether(8), erythro-guaiacylglycerol-8-O-4′-(sinapyl alcohol)ether(9), threo-syringylglycerol-8-O-4′-(sinapyl alcohol)ether(10), burselignan(11)and icariol A2(12). Compounds 1 to 12 were all isolated from this plant for the first time, and compound 1 was a new natural product.

Alkaloid constituents from Corydalis decumbens / 中国药科大学学报

To study alkaloid constituents in Corydalis decumbens,thirteen compounds were isolated from 95％ ethanol extracts of Corydalis decumbens (Thunb) Pers.by silica gel,RP-C1s,Sephadex LH-20 column chromatographer,recry stallization,thin-layer chromatography and HPLC.Their structures were elucidated on the basis of spectral data combined with physiochemical properties as tetrahydropalmatine (1),oxyhydrastinine (2),doryanine (3),palmatine (4),bicuculline (5),canadine (6),tetrahydrocoptisine(7),corydaldine (8),epicorynoxidine (9),N-methylcorydaldine (10),(+)-corlumine (11),N-methyl-6,7-dimethoxyisoquinolone (12) and oxysanguinarine (13).Compounds 2,3,6,7,and 9-13 were isolated from this plant for the first time.In addition,compounds 2,3,and 9-13 were obtained firstly from this genus.Pharmacological experiments showed that tetrahydropalmatine (1) might have analgesic or sedative effects,and the bicuculline (5) could probably induce epilepsy.

Research on the extracting process of alkannin from radix arnebiae seu lithospermi / 中国基层医药

Objective Optimizing the preparation technology of Red buttock ointment,by exploring the best extraction conditions of the alkannin,radix arnebiae seu lithospermi polysaccharides and so on.Methods Selecting radix arnebiae seu lithospermi 1kg and using orthogonal experiment to explore the best extraction conditions.Naphtho-quinone pigment chemicals were extracted by reflux extraction method;ethanol and paraffin wax were chosen as the composite solvent.Radix arnebiae seu lithospermi polysaccharide was extracted by water decocting,which to observe the rationality of process.Results The best extraction condition is A3 B2 C1 D1 , including section A:ethanol was 20 times the quality of medicinal materials (select radix arnebiae seu lithospermi 1kg and ethanol 20kg),section B:the amount of paraffin was 5% of ethanol(select radix arnebiae seu lithospermi 1kg and paraffin 1 kg),section C:Boiling water was 20 times the quality of medicinal materials,section D:the boiling time was 2h.Conclusion The more practical production process conditions:Ethanol was 10 times the quality of medicinal materials,and the amount of paraffin was 5%of ethanol.Boiling water was 10 times the quality of medicinal materials,and the boiling time was 1 h.This method is economic, simple, reasonable, stable, reproducible and easy to operation.Red buttock ointment obtained by this method possesses of excellent quality,good performance and superior effect.

Study on relativity of genetic variants of rs 12425791 on chromosome 12p13 and prognosis in patients with acute ischemic stroke / 重庆医学

Objective To investigate the association between genetic variants of rs 12425791 on chromosome 12p13 and the one year risk of stroke-related death or recurrent stroke following initial stroke .Methods A total of 401 acute ischemic stroke(AIS) patients who had first-ever ischemic stroke were analyzed .The genotypes of chromosome 12p13 rs12425791 were analyzed by PCR-RFLP .Logistic regression analysis was used to assess the effect of individual genotype on stroke-related mortality or recurrent stroke in one year .Results Among the 401 AIS patients ,31 died or developed a recurrent stroke .After adjustment for age ,sex and vascular risk factors ,logistic regression analysis indicated that the odd ratio were 2 .602(95% CI 1 .216-5 .564) for individuals with homozygous variant allele of rs12425791 .Conclusion This study found that genetic variants of rs12425791 on chromosome 12p13 are independent predictors of stroke-related mortality or stroke recurrence in patients with incident ischemic stroke .

Efficacy Observation of Shenfu Tongmai Capsule Combined with Xuesaitong Injection for Cerebral Infarction / 中国药房

OBJECTIVE:To explore the clinical efficacy of Shenfu tongmai capsule in the treatment of cerebral infarction.METHODS:204 patients were equally divided into 3 groups.Group A were given Shenfu tongmai capsule combined with Xuesaitong injection.Group B were treated with 40 mL Xuesaitong injection added into 250 mL 0.9%NaCl (i.v.gtt).Group C were orally administered Shenfu tongmai capsule (0.3 g?7,t.i.d).One treatment course lasted for 3 weeks.Blood rheology and platelet activation markers were detected by L-G-R80A fully automatic blood rheology analysis instrument and Epics XL-4 flow cytometry to analyze the clinical efficacy.All markers were redetected after half year.RESULTS: Group A was better than group B and C in the respect of overall cure rate,total effective rates,platelet activation markers and blood rheology (P0.05).CONCLUSION: Shenfu tongmai capsule combined with Xuesaitong injection might be one of optimal way in the treatment of cerebral infarction.Shenfu tongmai capsule especially suits the lower-income patients with cerebral infarction and rehabilitation.