Anti-tumor Effect and Mechanism of Effective Components in Hedysari Radix： A Review / 中国实验方剂学杂志

Malignant tumors are one of the main causes of human death worldwide and pose a serious threat to human health. The current treatment methods are mainly the combination of chemotherapeutics， surgery， radiotherapy， or hormone therapy. The treatment process has limitations such as multidrug resistance， non-selective targeting of cancer cells， and drug toxicity. With the development and application of traditional Chinese medicine （TCM）， Chinese medicine has the characteristics of multi-angle and multi-mechanism coordination and slight toxic and side effects. It can effectively inhibit tumor proliferation， differentiation， and metastasis， and avoid drug resistance， serving as the focus of current tumor treatment research. Hedysari Radix， one of the genuine medicinal materials in Gansu province， is a tonic Chinese medicine with a wide range of pharmacological effects such as anti-inflammation， immune regulation， anti-oxidation， prevention and treatment of diabetic complications. In the majority of the ancient works on herbs of the past dynasties， Hedysari Radix was included under the item of Astragali Radix and used as Astragali Radix. Hedysari Radix is superior to Astragali Radix in enhancing immunity， scavenging free radicals， and resisting liver fibrosis. Studies have found that the effective components of Hedysari Radix have a prominent anti-tumor effect and a significant inhibitory effect on various malignant tumors such as liver cancer， bladder cancer， gastric cancer， breast cancer， and colorectal cancer. They can also combine with clinical anti-cancer drugs to reduce the toxic and side effects of chemotherapy drugs and improve the tolerance of patients during chemotherapy. On the basis of current research， this study summarized the mechanism of Hedysari Radix active components in inducing cell apoptosis， blocking cell cycle， inhibiting tumor cell proliferation， migration， and invasion， regulating micro mRNA （miRNA）， inducing cell autophagy， enhancing immune regulation， as well as reducing toxicity and enhancing efficiency and sensitization with clinical chemotherapeutics， and systematically explained the anti-tumor mechanism of Hedysari Radix active components， aiming to provide a basic reference for the further exploration of the anti-tumor mechanism of Hedysari Radix and the further development and utilization of its effective components.

Optimization of the Extraction Technology of Total Flavonoids from the Roots and Stems of Angelica sinensis / 中国药房

OBJECTIVE: To optimize reflux extraction technology of total flavonoids from the roots and stems of Angelica sinensis. METHODS: The reflux extraction technology of total flavonoids from the roots and stems of A. sinensis was optimized by Box-Behnken design-response methodology based on single factor test using volume fraction of extraction solvent ethanol, solide-liquid ration, extraction time, extraction times as investigation factors, the content of total flavonoids in extract as evaluation index. RESULTS: The optimal extraction technology of total flavonoids from the roots and stems of A. sinensis was that volume fractions of ethanol were 70％ and 50％; solid-liquid ratios were 1: 40 and 1: 30; extraction time were 1. 3 h and 1. 7 h; The number of extraction times is two times. In verification test, the contents of total flavonoids were 7. 253 6, 25. 144 1 mg/g (RSD= 1. 57％, 1. 49％, n = 3); relative errors of those to predicted value (6. 942 8, 25. 703 5 mg/g) were 4. 28％, 2. 24％. CONCLUSIONS: Optimized extraction technology for total flavonoids from the roots and stems of A. sinensis is simple, reproducible and predictable.

Comprehensive Quality Evaluation of Gansu Baitiao Codonopsis Radix with Different Commercial Grades / 中国中医药信息杂志

ObjectiveTo compare the quality of Baitiao Codonopsis Radix with different commercial grades; To provide the basis for the rational utilization of Codonopsis Radix.MethodsTotally 54 batchs of Baitiao Codonopsis Radix with different commercial grades from main producing areas in Gansu were collected. The contents of lobetyolin and atractylenoideⅢ in Codonopsis Radix were detected by HPLC. Colorimetric method was used to detect the content of polysaccharide in the Codonopsis Radix. Chinese Pharmacopoeia 2010 Edition was referred to detect the content of extractum. One way ANOVA and factor analysis method were used to comprehensively evaluate the data.ResultsLobetyolin and atractylenoideⅢ were all negatively correlated with commercial grades. The results of the principal component factor ranking showed that the samples were not sorted by the order of commercial grades, with commercial grade two ranking in the forefront.Conclusion The quality of Baitiao Codonopsis Radix with different commercial grades is different, and the quality of commercial grade two is better than other commercial grades.

Study on Quality Evaluation of Hedysari Radix from Different Producing Areas in Gansu Province Based on the Contents of 12 Trace Elements / 中国中医药信息杂志

Objective To evaluate the quality of Hedysari Radix from different producing areas in Gansu Province by setting Hedysari Radix from different producing areas in Gansu Province as study subjects and trace elements in Hedysari Radix as evaluation indexes; To provide the references for establishment of the quality standards of Hedysari Radix.Methods The content of Fe, Cu, Ca, Mn, Zn, Mg, Cr, Co, Na, Li, Ni and K in Hedysari Radix were detected by using flame atomic absorbption method. Data were analyzed by the factor analysis conducted by SPSS21.0 software. The common factors were extracted by principal component and analytic function for quality comprehensive ranking was established.ResultsThree common factors were extracted, namely F1, F2, and F3. The quality function was Y=0.657F1+0.119F2+0.089F3. Cr, Li, Co, Cu, Zn, Fe and Mg were the characteristic trace elements of Hedysari Radix that could affect the quality of Hedysari Radix. When the quality of Hedysari Radix was evaluated by the contents of trace elements, the quality of wild Hedysari Radix were better than cultivated Hedysari Radix and the quality rank was consistent with the idea that Longnan is the main producing area of Hedysari Radix. The quality of samples from Dingxi area was better.Conclusion The quality evaluation of Hedysari Radix based on trace elements shows the differences in Hedysari Radix from different producing areas in Gansu Province and the results are relatively reasonable.

Study on Characteristics of Trace Elements of Hedysari Radix in Different Growth Ages / 中国中医药信息杂志

Objective To discuss the correlativity of the characteristics of trace elements and the quality in Hedysari Radix in different growth ages by taking the Hedysari Radix with different growth ages in Gansu as the research objects. Methods Hedysari Radix in different growth ages was collected. The contents of 12 trace elements in Hedysari Radix were detected by using flame atomic absorptions pectrophotometer.The multiple comparisons, correlation analysis, discriminant analysis and factor analysis were performed by SPSS21.0. Results The contents of Ni, Mg, and Mn were significant in Hedysari Radix with different growth ages. The growth ages had significantly negative correlation with Zn contents and had significantly positive correlation with Li contents. The contents of Cu, Fe, Ca, K, Zn, Na, and Mn and growth ages existed Fisher linear. The quality of Hedysari Radix with different growth ages had difference;the quality of Hedysari Radix with 2-year-old growth ages also had difference; the quality of 3 and 4-year-old Hedysari Radix was better than 6 and 8-year-old. Conclusion There are closely relationships between growth and the contents of certain trace elements. The quality of Hedysari Radix has some relationships with the contents of trace elements.

Characteristic Analysis on Trace Elements from Hedysari Radix in Different Months / 中国中医药信息杂志

Objective To analyze the characteristic changes of trace elements from Hedysari Radix in different months;To provide references for the choice of the best harvest periods. Methods Trace elements of Hedysari Radix from different months (June, July, August, September, October and November) were determined by atomic absorption spectroscopy and analyzed by single factors analysis of variance, correlation analysis, cluster analysis and factors analysis. Results There was obvious significance between the contents of Cu and Co (P<0.05), and there was obvious significance among the contents of Fe, Ca, Mn, Zn, Mg, Cr, Na, Li, Ni and K (P<0.01). The results of correlation analysis showed that months had positive correlation with Cu, had a negative correlation with Fe, Na, Mn, Cr and Li contents, and had significantly negative correlation with Ca, Mg contents. Mn, Cr, Ni, K and Na were the characteristics of trace elements from Hedysari Radix in different months. The different months samples could be divided into two categories of June, July, August, September samples (Ⅰ) and October, November samples (Ⅱ). Conclusion There is close relationship between the contents of trace elements from Hedysari Radix and months. The information and metabolic patterns of trace elements from Hedysari Radix in June, July, August, September (immature) samples are close or similar;the information of trace elements in October, November (nearly mature or maturing) samples are close or similar. Study on harvest times of Hedysari Radix by trace element as an indicator is with certain rationality.

Study on the Correlation between Contents of Active Ingredients in Hedysarum polybotrys from Gansu and Ecological Factors / 中国药房

ABSTRCT OBJECTIVE:To analyze the correlation between contents of active ingredients in Hedysarum polybotrys and ecologi-cal factors. METHODS:HPLC was used for the simultaneous determination of calycosin and formononetin in H. polybotrys;UV spectrophotometry was used to determine the content of hedysari polysaccharide;and hot-dip method was used to determine the con-tent of extracts in H. polybotrys. Factor analysis method was used to analyze the correlation between contents of active ingredients and ecological factors. RESULTS:4 common factors,which were heat,geography,illumination and precipitation,were extracted. The heat was an ecological dominant factor influencing the quality of H. polybotrys. And ≥10 ℃ accurnulated temperature was the most significant variate influencing hedysari polysaccharide,latitude was the most significant variate influencing the contents of ca-lycosin and formononetin H. polybotrys;and annual averge amount of radiation was the most significant variate influencing content of extracts in H. polybotrys. CONCLUSIONS:Different ecological factors have different effects on the contents of active ingredi-ents in H. polybotrys,and it is the result of the combined effect of various of ecological factors.